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COG2 negatively regulates chilling tolerance through cell wall components altered in rice

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Abstract

Key message

Chilling-tolerant QTL gene COG2 encoded an extensin and repressed chilling tolerance by affecting the compositions of cell wall.

Abstract

Rice as a major crop is susceptible to chilling stress. Chilling tolerance is a complex trait controlled by multiple quantitative trait loci (QTLs). Here, we identify a QTL gene, COG2, that negatively regulates cold tolerance at seedling stage in rice. COG2 overexpression transgenic plants are sensitive to cold, whereas knockout transgenic lines enhance chilling tolerance. Natural variation analysis shows that Hap1 is a specific haplotype in japonica/Geng rice and correlates with chilling tolerance. The SNP1 in COG2 promoter is a specific divergency and leads to the difference in the expression level of COG2 between japonica/Geng and indica/Xian cultivars. COG2 encodes a cell wall-localized extensin and affects the compositions of cell wall, including pectin and cellulose, to defense the chilling stress. The results extend the understanding of the adaptation to the environment and provide an editing target for molecular design breeding of cold tolerance in rice.

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Acknowledgements

We are grateful to Dr. Jingquan Li (Institute of Botany, Chinese Academy of Sciences) for her excellent technical assistance with confocal microscopy.

Funding

This work was supported by the Strategic Priority Research Program of the Chinese Academy of Science (Grant No. XDA24010301) and the Basic Science Center Project of National Natural Science Foundation of China (Grant No. 31788103).

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Authors and Affiliations

  1. The Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China

    Jinglei Feng, Zhitao Li, Wei Luo, Yunyuan Xu & Kang Chong

  2. University of the Chinese Academy of Sciences, Beijing, 100049, China

    Jinglei Feng, Zhitao Li & Kang Chong

  3. The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100093, China

    Yunyuan Xu & Kang Chong

  4. Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Centre for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, 225009, China

    Guohua Liang

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  1. Jinglei Feng
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Contributions

JLF designed the research, performed the experiments, and wrote the manuscript. ZTL and WL performed the experiments and data analysis. GHL, YYX and KC designed the research and wrote the manuscript.

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Correspondence to Kang Chong.

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Feng, J., Li, Z., Luo, W. et al. COG2 negatively regulates chilling tolerance through cell wall components altered in rice. Theor Appl Genet 136, 19 (2023). https://doi.org/10.1007/s00122-023-04261-w

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