Abstract
Key message
Here, we report identification of a large effect QTL conferring Mungbean yellow mosaic India virus resistance introgressed from ricebean in blackgram variety Mash114. The tightly linked KASP markers would assist in marker-assisted-transfer of this region into Vigna species infected by MYMIV.
Abstract
Until recently, precise location of genes and marker-assisted selection was long thought in legumes such as blackgram due to lack of dense molecular maps. However, advances in next-generation sequencing based on high-throughput genotyping technologies such as QTL-seq have revolutionized trait mapping in marker-orphan crops. Using QTL-seq approach, we have identified a large-effect QTL for resistance to Mungbean yellow mosaic India virus (MYMIV) in blackgram variety Mash114. MYMIV is devastating disease responsible for huge yield losses in blackgram, greengram and other legumes. Mash114 showed consistent and high level of resistance to MYMIV since last nine years. Whole genome re-sequencing of MYMIV-resistant and susceptible bulks derived from RILs of cross KUG253 X Mash114 identified a large-effect QTL (qMYMIV6.1.1) spanning 3.4 Mb on chromosome 6 explaining 70% of total phenotypic variation. This region was further identified as an inter-specific introgression from ricebean. Linkage mapping using KASP markers developed from potent candidate genes involved in virus resistance identified the 500 kb genomic region equaling 1.9 cM on genetic map linked with MYMIV. The three KASP markers closely associated with MYMIV originated from serine threonine kinase, UBE2D2 and BAK1/BRI1-ASSOCIATED RECEPTOR KINASE genes. These KASPs can be used for marker-assisted transfer of introgressed segment into suitable backgrounds of Vigna species.
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Data availability
All the original data are available in NCBI SRA database with accession number PRJNA788646.
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Acknowledgements
We acknowledge efforts of Dr. Inderjit Singh, Dr J. S. Sandhu, Dr. S. Gupta and Dr. Sarvjeet Singh for developing the genotype, ‘Mash114.’
Funding
The funding of research was supported by Department of Biotechnology under project entitled as ‘Genetic Enhancement of Minor Pulses: Characterization, Evaluation, Genetic Enhancement and Generation of Genomic Resources for Accelerated Utilization and Improvement of Minor Pulses.’
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SKD performed experiments and contributed to writing of manuscript; SK and RKG conceptualized the work and edited manuscript and finalized it; SK, AS and DB assisted in carrying out experiments; and DB and AK contributed in manuscript editing.
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Dhaliwal, S.K., Gill, R.K., Sharma, A. et al. A large-effect QTL introgressed from ricebean imparts resistance to Mungbean yellow mosaic India virus in blackgram (Vigna mungo (L.) Hepper). Theor Appl Genet 135, 4495–4506 (2022). https://doi.org/10.1007/s00122-022-04234-5
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DOI: https://doi.org/10.1007/s00122-022-04234-5