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Golden 2-like transcription factor contributes to the major QTL against rice black-streaked dwarf virus disease

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Abstract

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A major resistance QTL was identified on chromosome 6 in rice variety Wuke; both overexpression and knockdown experiments confirmed that OsGLK1 is the candidate gene for association with Rice black-streaked dwarf virus disease.

Abstract

Rice black-streaked dwarf virus disease is one of the most destructive rice viral diseases in China and East Asia. Progress has been limited in RBSDVD resistance breeding due to inadequate knowledge on the underlying functional genes. In this study, a major QTL for RBSDV (rice black-streaked dwarf virus) independent of SBPH (small brown planthopper) resistance was mapped in a 1.8 Mb interval on chromosome 6 by using an F2:3 population originated from resistant rice variety Wuke. Representative transcripts within this region were analysed and three genes showing amino acid sequence variation in functional domains were selected for transformation. Overexpression experiments showed that one gene exhibited significant enhanced resistance compared to control lines, encoding protein involving Myb domain and probable transcription factor Golden 2-like1 (GLK1). Furthermore, OsGLK1 knockdown rice lines were investigated and the resistance ability was significantly declined without this gene compared to the wild type. Taken together, both overexpression and knockdown experiments strongly suggested that OsGLK1 plays an important role for RBSDV resistance and contributes to the major QTL. The study paves the way for elucidating the molecular mechanism underlying RBSDVD resistance and the molecular markers associated with OsGLK1 may be used for marker-assisted selection.

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Data availability

The plant materials and datasets employed in this study are available from the corresponding author on reasonable request.

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Acknowledgements

Rice variety Wuke was kindly provided by Prof. Xianwen Fang, Dongjin and Osglk1 were kindly provided by Jane A. Langdale’s laboratory.

Funding

This research was supported by National Natural Science Foundation of China (31761143012, 31901959); Open Competition Mechanism to Select the Best Candidates Fund of Jiangsu Province (JBGS[2021]008); Key Research and Development Program of Jiangsu Province (BE2022369); Agricultural Science and Technology Independent Innovation Fund of Jiangsu Province (CX(19)1008).

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Authors and Affiliations

  1. Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu Province, China

    Xuejuan Li, Feng Lin, Chenyang Li, Linlin Du, Wenjuan Shi, Jianying Lv, Xiaoyan Cao, Ying Lan, Yongjian Fan, Yijun Zhou & Tong Zhou

  2. International Rice Research Institute and Jiangsu Academy of Agricultural Sciences Joint Laboratory, NanjingJiangsu Province, 210014, China

    Feng Lin & Tong Zhou

  3. Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu Province, China

    Zhiyang Liu

  4. Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China

    Xuejuan Li, Wenjuan Shi, Jianying Lv, Xiaoyan Cao & Tong Zhou

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  1. Xuejuan Li
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Contributions

TZ, YZ, YF conceived the research programme, XL, FL, CL carried out transformation and quantitative RT-PCR, LD, ZL, WS did QTL mapping, JL, XC collected phenotypic data; YL conducted data analyses; FL wrote the manuscript.

Corresponding author

Correspondence to Tong Zhou.

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The authors declare no conflict of interest.

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Communicated by Jiankang Wang.

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Xuejuan Li, Feng Lin, Chenyang Li, these authors contributed equally to this work.

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Li, X., Lin, F., Li, C. et al. Golden 2-like transcription factor contributes to the major QTL against rice black-streaked dwarf virus disease. Theor Appl Genet 135, 4233–4243 (2022). https://doi.org/10.1007/s00122-022-04214-9

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