Abstract
Key message
A GST for red-spot-petals in Gossypium arboreum was identified as the candidate under the scope of multi-omics approaches.
Abstract
Colored petal spots are correlated with insect pollination efficiency in Gossypium species. However, molecular mechanisms concerning the formation of red spots on Gossypium arboreum flowers remain elusive. In the current study, the Shixiya1-R (SxyR, with red spots) × Shixiya1-W (SxyW, without red spots) segregating population was utilized to determine that the red-spot-petal phenotype was levered by a single dominant locus. This phenotype was expectedly related to the anthocyanin metabolites, wherein the cyanidin and delphinidin derivatives constituted the major partition. Subsequently, this dominant locus was narrowed to a 3.27 Mb range on chromosome 7 by genomic resequencing from the two parents and the two segregated progeny bulks that have spotted petals or not. Furthermore, differential expressed genes generated from the two bulks at either of three sequential flower developmental stages that spanning the spot formation were intersected with the annotated ones that allocated to the 3.27 Mb interval, which returned eight genes. A glutathione S-transferase-coding gene (Gar07G08900) out of the eight was the only one that exhibited simultaneously differential expression among all three developmental stages, and it was therefore considered to be the probable candidate. Finally, functional validation upon this candidate was achieved by the appearance of scattered petal spots with inhibited expression of Gar07G08900. In conclusion, the current report identified a key gene for the red spotted petal in G. arboreum under the scope of multi-omics approaches, such efforts and embedded molecular resources would benefit future applications underlying the flower color trait in cotton.
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Data availability
Genomic resequencing data were deposited into Sequence read archive (SRA) database under the accession numbers of SAMN28187015 to SAMN28187018, and the transcriptome data were under the accession numbers of SAMN28185984 to SAMN28185989.
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This work was supported by HAAFS Science and Technology Innovation Special Project (2022KJCXZX-MHS-1) and Youth Fund of Hebei Natural Science Foundation (C2021301039).
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JZ, XZ and SZ conceived and designed the experiments. SZ, XC, HW, LT and XL performed the experiments. JC and TJ analyzed the data. JC and SZ wrote the paper. All authors read and approved the final version of the paper.
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122_2022_4191_MOESM3_ESM.xlsx
Table S1 Primers used in the current study. Table S2 Chromosomal distribution of the SNPs and InDels. Table S3 Candidate genes involved in the chromosomal intervals mapped by the Euclidean distance and SNP-index association algorithms. Table S4 DEGs at stage II.Table S5 DEGs at stage III. Table S6 DEGs at stage IV. (XLSX 76 KB)
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Zhang, S., Chen, J., Jiang, T. et al. Genetic mapping, transcriptomic sequencing and metabolic profiling indicated a glutathione S-transferase is responsible for the red-spot-petals in Gossypium arboreum. Theor Appl Genet 135, 3443–3454 (2022). https://doi.org/10.1007/s00122-022-04191-z
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DOI: https://doi.org/10.1007/s00122-022-04191-z