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Fine mapping and candidate gene analysis of gummy stem blight resistance in cucumber stem

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Abstract

Key message

Two candidate genes (Csa6G046210 and Csa6G046240) were identified by fine-mapping gsb-s6.2 for gummy stem blight resistance in cucumber stem.

Abstract

Gummy stem blight (GSB) is a serious fungal disease caused by Didymella bryoniae, that affects cucumber yield and quality worldwide. However, no GSB-resistant genes have been identified in cucumber cultivars. In this study, the wild cucumber accession ‘PI 183967’ was used as a source of resistance to GSB in adult stems. An F2 population was mapped using resistant line ‘LM189’ and susceptible line ‘LM6’ derived from a cross between ‘PI 183967’ and ‘931’. By developing InDel and SNP markers, the gsb-s6.2 QTL on Chr. 6 was fine-mapped to a 34 kb interval harboring six genes. Gene Expression analysis after inoculation showed that two candidate genes (Csa6G046210 and Csa6G046240) were induced and differentially expressed between the resistant and susceptible parents, and may be involved in disease defense. Sequence alignment showed that Csa6G046210 encodes a multiple myeloma tumor-associated protein, and it harbored two nonsynonymous SNPs and one InDel in the third and the fourth exons, and two InDels in the TATA-box of the basal promoter region. Csa6G046240 encodes a MYB transcription factor with six variants in the AP2/ERF and MYB motifs in the promoter. These two candidate genes lay the foundation for revealing the mechanism of GSB resistance and may be useful for marker-assisted selection in cucumber disease-resistant breeding.

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Data availability

All reference data that are not presented in this manuscript are available in the supplemental tables. All materials are available to the public upon request and under material transfer agreement.

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Funding

This work was supported by Key-Area Research and Development Program of Guangdong Province (2020B020220001), Key-Area Research and Development Program of Shandong Province (2021LZGC016), the earmarked fund for Modern Agro-industry Technology Research System (CARS-23), the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS), Central Public-Interest Scientific Institution Basal Research Fund (No.Y2017PT52), and the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture and Rural Affairs, P. R. China.

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Author notes

  1. Jianan Han and Shaoyun Dong these authors contributed equally to this work.

Authors and Affiliations

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Jianan Han, Shaoyun Dong, Yanxia Shi, Han Miao, Xiaoping Liu, Xingfang Gu & Shengping Zhang

  2. Department of Plant Sciences, University of California, Davis, One Shield Avenue, Davis, CA, 95616, USA

    Diane M. Beckles

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  1. Jianan Han
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  2. Shaoyun Dong
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  3. Yanxia Shi
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  4. Han Miao
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  7. Xingfang Gu
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Contributions

G.X.F. and Z.S.P. designed the experiments, H.J.N. performed the experiments, analyzed the data, and wrote the manuscript. D.S.Y., L.X.P., D.M.B. and Z.S.P. revised the manuscript. M.H participated partial experiments. S.Y.X. provided Didymella bryoniae. All authors read and approved the manuscript.

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Correspondence to Xingfang Gu or Shengping Zhang.

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Communicated by Sandra Elaine Branham.

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Han, J., Dong, S., Shi, Y. et al. Fine mapping and candidate gene analysis of gummy stem blight resistance in cucumber stem. Theor Appl Genet 135, 3117–3125 (2022). https://doi.org/10.1007/s00122-022-04172-2

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  • DOI: https://doi.org/10.1007/s00122-022-04172-2

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