Abstract
Key message
New translocation lines with T6V#4S·6AL in the Ph1 and ph1b backgrounds were developed with improved inheritance of powdery mildew resistance.
Abstract
The wheat-Dasypyrum villosum T6V#4S·6DL translocation line Pm97033, which exhibits strong powdery mildew (PM) resistance, was developed many years ago, but has limited application in wheat breeding. One of the major reasons for this is that the translocation chromosome has low transmission rate, which makes it difficult to obtain ideal genotype through recombination with other elite agronomic traits in a limited segregating population. Further modifications are thus needed to make better use of this genetic resource. In this study, Pm97033 and the T6V#2S·6AL translocation line NY-W were hybridized with the CS ph1b mutant, and two F1 hybrids were hybridized with each other. Then, plants homozygous for the ph1b deletion carrying the alien chromosome arm(s) 6V#2S and 6V#4S were identified from the segregating populations using molecular markers. New T6V#4S·6AL and T6V#2-6V#4S·6AL translocations were identified by molecular markers and confirmed by genomic in situ hybridization (GISH). Individuals that were heterozygous or homozygous for the translocation chromosome in Ph1 and ph1b backgrounds were obtained. The ratio of PM resistance vs. susceptibility in the self-pollinated heterozygous plants was 3:1, and the phenotype was completely consistent with the KASP genotyping. Thus, the new translocation chromosomes had higher transmission rate than the original T6V#4S·6DL, and so can be effectively applied in breeding programs.
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Acknowledgements
The authors thank Dr. Fangpu Han, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, for his guidance on genomic in situ hybridization of wheat chromosomes, and Ms. Yiping Yan, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, for her assistance in making hybridization and disease resistance investigation.
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This study was financially supported by the National Key Research and Development Program of China (Grant Nos. 2016YFD0102002 and 2016YFD0102001).
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XLM, YNC, and JNC conducted the experiments. XLM and ZSL analyzed the data. XLM, ZSL, and XGY wrote the manuscript. YNC prepared the files of figures and tables. MY and BCW participated in partial ph1b genotyping experiments. ZSL and XGY conceived and directed the project.
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Ma, X., Chang, Y., Chen, J. et al. Development of wheat-Dasypyrum villosum T6V#4S·6AL translocation lines with enhanced inheritance for powdery mildew resistance. Theor Appl Genet 135, 2423–2435 (2022). https://doi.org/10.1007/s00122-022-04124-w
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DOI: https://doi.org/10.1007/s00122-022-04124-w