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Fine mapping of the tiller inhibition gene TIN4 contributing to ideal plant architecture in common wheat

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Abstract

Key message

A tiller inhibition gene, TIN4, was mapped to an approximately 311 kb genomic interval on chromosome arm 2DL of wheat.

Abstract

The tiller is one of the key components of plant morphological architecture and a central agronomic trait affecting spike number in wheat. Low tiller number has been proposed as a major component of crop ideotypes for high yield potential. In this study, we characterized the development of tillering in near-isogenic lines (NIL7A and NIL7B), indicating that the TIN4 gene inhibited the growth of tillering buds and negatively regulated tiller number. Low-tillering was controlled by a single gene (TIN4) located on chromosome 2DL by genetic analysis and bulked segregant RNA-seq analysis. A total of 17 new polymorphic markers were developed in this study, and 61 recombinants were identified in the secondary F2 population containing 4,266 individuals. TIN4 was finally mapped on a 0.35 cM interval, co-segregated with molecular marker M380, within a 311 kb genomic interval of the wheat cultivar Chinese Spring reference genome sequence that contained twelve predicted genes. Yield experiments showed that the yield of low-tillering lines was higher than that of high-tillering lines at a higher density. Overall, this study provides a foundation for the construction of a low-tillering ideotype for improving wheat yield and further cloning TIN4 by map-based cloning approach.

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Data Availability

The sequencing datasets in this study can be accessed at the National Center for Biotechnology Information (NCBI) SRA repository under accession number PRJNA705399 and PRJNA657689.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (31771794), and the Key Research and Development Program of Sichuan Province (2021YFN0034 and 21ZDYF3575).

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Author notes

  1. Zhiqiang Wang and Fangkun Wu contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang, Chengdu, 611130, China

    Yaxi Liu

  2. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China

    Zhiqiang Wang, Fangkun Wu, Xudong Chen, Wanlin Zhou, Haoran Shi, Yu Lin, Shuai Hou, Shifan Yu, Hong Zhou, Caixia Li & Yaxi Liu

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  1. Zhiqiang Wang
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  2. Fangkun Wu
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Contributions

ZQW and FKW drafted and revised the manuscript, and mainly responsible for data analysis; XDC, WLZ, YL, SH, SFY and HZ performed the experiments; HRS and CXL participated in data analysis; YXL conceived and coordinated the project, and revised the manuscript; and all authors read and approved the final manuscript for publication.

Corresponding author

Correspondence to Yaxi Liu.

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All authors declare that there is no conflict of interest.

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The authors declare that the experiments comply with the current laws of the country in which they were performed.

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Communicated by Takao Komatsuda.

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Wang, Z., Wu, F., Chen, X. et al. Fine mapping of the tiller inhibition gene TIN4 contributing to ideal plant architecture in common wheat. Theor Appl Genet 135, 527–535 (2022). https://doi.org/10.1007/s00122-021-03981-1

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