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A genome-wide association study reveals that the cytochrome b5 involved in seed reserve mobilization during seed germination in rice

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A candidate gene cytochrome b5 for the major QTL qSRMP9 for rice seed reserve mobilization was validated during seed germination using a genome-wide association study approach.

Abstract

Seed reserve mobilization plays important roles in the early seedling growth in rice. However, the genetic basis underlying this process is poorly understood. In this study, the genetic architecture of variation in seed reserve mobilization during seed germination was studied using a genome-wide association study approach in rice. Three quantitative trait loci (QTL) including qSRMP6, qSRMP9, and qSRMP12 for seed reserve mobilization percentage were identified. In which, the candidate gene cytochrome b5 (OsCyb5) for the major QTL qSRMP9 was validated. Disruption of this gene in Oscyb5 mutants reduced the seed reserve mobilization and seedling growth compared with wild-type (WT) in rice. There were no significant differences of grain size, starch, protein and total soluble sugar content in the mature grains between Oscyb5 mutants and WT. However, the α-amylase activity in the germinating seeds of Oscyb5 mutants was significantly decreased compared to that of WT, and then, the starch and sugar mobilization and the glucose accumulation during seed germination were significantly decreased in Oscyb5 mutants. Two elite haplotypes of OsCyb5 associated with the higher seed reserve mobilization percentage and its elite single nucleotide polymorphism variations were mainly existed in the INDICA and AUS accessions. The natural variation of OsCyb5 contributing to seed reserve mobilization might be useful for the future rice breeding.

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Acknowledgements

The authors would like to thank USDA-ARS for seeds of the Rice Diversity Panel. This work was supported by the National Natural Science Foundation of China (Grant No. 32172052, 31971995, 31901601, and 31771889), the Natural Science Foundation of Guangdong Province (Grant No. 2020A1515011053), the Key-Area Research and Development Program of Guangdong Province (Grant No. 2018B020202012 and 2020B020219004), the Science and Technology Support Program of Huzhou (2018GZ07) and the startup funding from South China Agricultural University.

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  1. Zhibo Huang and Jifeng Ying contributed equally to this work.

Authors and Affiliations

  1. The Laboratory of Seed Science and Technology, Guangdong Key Laboratory of Plant Molecular Breeding, Guangdong Laboratory of Lingnan Modern Agriculture, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China

    Zhibo Huang, Liling Peng, Shan Sun, Chengwei Huang, Can Li, Zhoufei Wang & Yongqi He

  2. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China

    Jifeng Ying

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  1. Zhibo Huang
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  2. Jifeng Ying
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  4. Shan Sun
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  5. Chengwei Huang
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  6. Can Li
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Contributions

ZW and JF planned the research. ZH and YH performed all important experiments. LP, SS, and CH performed physiological assay. CL performed seed germination experiments. ZW, YH, and ZH analyzed the data and wrote the paper.

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Correspondence to Zhoufei Wang or Yongqi He.

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The authors declare no conflict of interest.

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Communicated by Takuji Sasaki.

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Huang, Z., Ying, J., Peng, L. et al. A genome-wide association study reveals that the cytochrome b5 involved in seed reserve mobilization during seed germination in rice. Theor Appl Genet 134, 4067–4076 (2021). https://doi.org/10.1007/s00122-021-03948-2

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