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Characterization of a new gene for resistance to wheat powdery mildew on chromosome 1RL of wild rye Secale sylvestre

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PmSESY, a new wheat powdery mildew resistance gene was characterized and genetically mapped to the terminal region of chromosome 1RL of wild rye Secale sylvestre.

Abstract

The genus Secale is an important resource for wheat improvement. The Secale species are usually considered as non-adapted hosts of Blumeria graminis f. sp. tritici (Bgt) that causes wheat powdery mildew. However, as a wild species of cultivated rye, S. sylvestre is rarely studied. Here, we reported that 25 S. sylvestre accessions were susceptible to isolate BgtYZ01, whereas the other five confer effective resistance to all the tested isolates of Bgt. A population was then constructed by crossing the resistant accession SESY-01 with the susceptible accession SESY-11. Genetic analysis showed that the resistance in SESY-01 was controlled by a single dominant gene, temporarily designated as PmSESY. Subsequently, combining bulked segregant RNA-Seq (BSR-Seq) analysis with molecular analysis, PmSESY was mapped into a 1.88 cM genetic interval in the terminus of the long arm of 1R, which was closely flanked by markers Xss06 and Xss09 with genetic distances of 0.87 cM and 1.01 cM, respectively. Comparative mapping demonstrated that the corresponding physical region of the PmSESY locus was about 3.81 Mb in rye cv. Lo7 genome, where 30 disease resistance-related genes were annotated, including five NLR-type disease resistance genes, three kinase family protein genes, three leucine-rich repeat receptor-like protein kinase genes and so on. This study gives a new insight into S. sylvestre that shows divergence in response to Bgt and reports a new powdery mildew resistance gene that has potential to be used for resistance improvement in wheat.

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Acknowledgements

This study was supported by grants from Jiangsu Agricultural Science and Technology Innovation Fund [CX(19)2042], State Key Laboratory of Plant Cell and Chromosome Engineering (PCCE-KF-2019-04), Priority Academic Program Development of Jiangsu Higher Education Institutions, Jiangsu Education Department, National Natural Science Foundation of China (31872009), Leading Talents Plan of Hubei Academy of Agricultural Sciences (L2018013), Taishan Scholars Project (tsqn201812123) and State Key Laboratory of Crop Biology in Shandong Agricultural University (2020KF07). The authors are thankful to National Centre for Plant Genetic Resources, Polish Genebank (NCPGR), Genebank Information System of the IPK Gatersleben (GBIS-IPK) and Germplasm Resources Information Network (GRIN) for providing Secale sylvestre accessions. The authors are also grateful to Prof. Robert McIntosh (The University of Sydney, Australia) for constructive comments on this manuscript and Prof. Hongjie Li (Chinese Academy of Agricultural Sciences, China) for editing this manuscript.

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Authors and Affiliations

  1. School of Life Sciences, Jiangsu University, Zhenjiang, 212013, China

    Huagang He & Tianlei Liu

  2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China

    Huagang He, Haonan Du, Renkang Liu & Weihong Sun

  3. School of Environment, Jiangsu University, Zhenjiang, 212013, China

    Huagang He & Shanying Zhu

  4. Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan, 430064, China

    Lijun Yang & Shuangjun Gong

  5. College of Agronomy, Sichuan Agricultural University, Chengdu, 611130, China

    Zongxiang Tang & Haimei Du

  6. Crop Research Institution, Shandong Academy of Agricultural Sciences, Jinan, 250100, China

    Cheng Liu & Ran Han

  7. INDEL Biological Technology Corporation, Nanjing, 210000, China

    Lei Wang

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  1. Huagang He
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Contributions

HH and SZ conceived and designed the experiments. HH, HD, RL, TL, LY, SG, ZT, HD, CL, RH, WS, and LW performed the experiments. HH and SZ analyzed the data and wrote the paper.

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Correspondence to Huagang He or Shanying Zhu.

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Communicated by Beat Keller.

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He, H., Du, H., Liu, R. et al. Characterization of a new gene for resistance to wheat powdery mildew on chromosome 1RL of wild rye Secale sylvestre. Theor Appl Genet 134, 887–896 (2021). https://doi.org/10.1007/s00122-020-03739-1

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