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Characterization of Pm68, a new powdery mildew resistance gene on chromosome 2BS of Greek durum wheat TRI 1796

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New powdery mildew resistance gene Pm68 was found in the terminal region of chromosome 2BS of Greek durum wheat TRI 1796. The co-segregated molecular markers could be used for MAS.

Abstract

Durum wheat (Triticum turgidum L. var. durum Desf.) is not only an important cereal crop for pasta making, but also a genetic resource for common wheat improvement. In the present study, a Greek durum wheat TRI 1796 was found to confer high resistance to all 22 tested isolates of Blumeria graminis f. sp. tritici (Bgt). Inheritance study on the F1 plants and the F2 population derived from the cross TRI 1796/PI 584832 revealed that the resistance in TRI 1796 was controlled by a single dominant gene, herein designated Pm68. Using the bulked segregant RNA-Seq (BSR-Seq) analysis combined with molecular analysis, Pm68 was mapped to the terminal part of the short arm of chromosome 2B and flanked by markers Xdw04 and Xdw12/Xdw13 with genetic distances of 0.22 cM each. According to the reference genome of durum wheat cv. Svevo, the corresponding physical region spanned the Pm68 locus was about 1.78-Mb, in which a number of disease resistance-related genes were annotated. This study reports the new powdery mildew resistance gene Pm68 that would be a valuable resource for improvement of both common wheat and durum wheat. The co-segregated markers (Xdw05–Xdw11) developed here would be useful tools for marker-assisted selection (MAS) in breeding.

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Acknowledgements

This study was supported by grants from Jiangsu Agricultural Science and Technology Innovation Fund [CX(19)2042], Priority Academic Program Development of Jiangsu Higher Education Institutions, Jiangsu Education Department, Key Research and Development Program of Yantai City (2019YT06000470), National Natural Science Foundation of China (31872009), Leading Talents Plan of Hubei Academy of Agricultural Sciences (L2018013) and State Key Laboratory of Crop Biology in Shandong Agricultural University (2020KF07). The authors are thankful to Genebank Information System of the IPK Gatersleben (GBIS-IPK) and Germplasm Resources Information Network (GRIN) for providing durum wheat accessions, and Prof. Zhiyong Liu (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, China) for providing the wheat lines carrying Pm26 and Pm42. The authors are also grateful to Prof. Robert McIntosh (The University of Sydney, Australia) for constructive comments on this manuscript.

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  1. Huagang He, Renkang Liu and Pengtao Ma have contributed equally to this work.

Authors and Affiliations

  1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China

    Huagang He, Renkang Liu, Haonan Du, Tianlei Liu & Shanying Zhu

  2. School of Environment, Jiangsu University, Zhenjiang, 212013, China

    Huagang He, Huanhuan Zhang & Shanying Zhu

  3. College of Life Sciences, Yantai University, Yantai, 264005, China

    Pengtao Ma

  4. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Qiuhong Wu

  5. Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan, 430064, China

    Lijun Yang & Shuangjun Gong

  6. USDA-ARS Western Regional Research Center, 800 Buchanan Street, Albany, CA, 94710, USA

    Huagang He, Naxin Huo, Yong Q. Gu & Shanying Zhu

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  1. Huagang He
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Contributions

HH, YQG and SZ conceived and designed the experiments. HH, RL, PM, HD, HZ, QW, LY, SG and TL performed the experiments. HH, RL, PM and NH analyzed the data. HH, RL, YQG and SZ wrote the paper.

Corresponding authors

Correspondence to Huagang He, Yong Q. Gu or Shanying Zhu.

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Communicated by Evans Lagudah.

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Supplementary Table S1

Information of 23 disease resistance-related genes in different genomes of wheat species. - means no matched gene or sequence. (XLSX 14 kb)

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He, H., Liu, R., Ma, P. et al. Characterization of Pm68, a new powdery mildew resistance gene on chromosome 2BS of Greek durum wheat TRI 1796. Theor Appl Genet 134, 53–62 (2021). https://doi.org/10.1007/s00122-020-03681-2

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