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Correction to: Theor Appl Genet (2015) 128:1605–1615 https://doi.org/10.1007/s00122-015-2535-8
The above-mentioned article was published in 2015 with an error in the reverse primer sequence for the PPOA2d1074 marker, which made amplification difficult. The reverse primer was missing a thymine nucleotide at the thirteenth position (GCGGTGCTTCACTTGGT). The corrected marker can be found in the following table. Additionally, a new primer set that provides a shorter, more abundant PCR product was designed and validated. The new marker is designated PPOA2d1074_2.
Marker | Gene | Forward (5′–3′) | Reverse (5′–3′) | Amplicon size (bp) |
|---|---|---|---|---|
PPOA2d1074 | Ppo-A2d | CATCATGTACCGCCAGATGG | GCGGTGCTTCACTTGGT | 878 |
PPOA2d1074_2 | Ppo-A2d | ACTTCACCGACACTGAC | TCACTTGGTATAGCCGATGC | 595 |
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Hystad, S.M., Martin, J.M., Graybosch, R.A. et al. Correction to: Genetic characterization and expression analysis of wheat (Triticum aestivum) line 07OR1074 exhibiting very low polyphenol oxidase (PPO) activity. Theor Appl Genet 133, 365 (2020). https://doi.org/10.1007/s00122-019-03459-1
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DOI: https://doi.org/10.1007/s00122-019-03459-1