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Fine mapping Ruv2, a new rust resistance gene in cowpea (Vigna unguiculata), to a 193-kb region enriched with NBS-type genes

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Abstract

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A new rust resistance gene Ruv2 was fine-mapped in cowpea to a 193-kb region on chromosome 2, which harboured 23 predicted gene models enriched with NBS-type genes.

Abstract

ZN016 is a landrace vegetable cowpea highly resistant to rust. Two previous studies using mixed-spores inoculation suggested different modes of inheritance of rust resistance in ZN016. In this study, we initially developed a detached leaf assay with a purified single-rust isolate (Auv-LS). Using this approach, we assessed the inheritance of rust resistance in a recombinant inbred line (RIL) population and an F2 population, both derived from the cross of “ZN016” and the susceptible cultivar “Zhijiang282.” A single dominant gene mode against Auv-LS was revealed in both populations. QTL mapping showed that this gene was coincident with the Ruv2 locus on LG7, one of the three resistance QTLs previously mapped based on mixed-spores inoculation data. Therefore, Ruv2 was considered as specifically against the rust isolate Auv-LS. Through an analysis of the RIL recombinants at Ruv2, we fine-mapped the gene to an ~ 0.45-cM interval between SNP markers 2_09656 and 2_00973, which corresponded to an ~ 193-kb region on chromosome 2 that harboured 23 predicted gene models enriched with NBS-type genes. Re-sequencing of the two parents revealed polymorphisms in four genes predictively to cause substantial protein structural changes, rendering them valuable candidate genes for future validation. Cross-species syntenic analysis indicated that Ruv2 may represent a novel rust resistance gene in food legumes. A cleaved amplified polymorphic sequences marker tightly linked to Ruv2 was developed to facilitate breeding. This work establishes a basis for map-based cloning of Ruv2 and breeding for rust resistance in cowpea and other legume crops.

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Acknowledgments

This study was partially supported by the Public Research Project of Zhejiang Province (LGN18C150009), the Major Science and Technology Project of Plant Breeding in Zhejiang Province (2016C02051-7-3) and the National Program for Support of Top-notch Young Professionals (to P.X.). We wish to thank Ye Tao (Biozeron Co. Ltd, Shanghai) for technical support in bioinformatic analysis of re-sequencing data.

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Authors and Affiliations

  1. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People’s Republic of China

    Xinyi Wu, Guojing Li, Baogen Wang, Yaowen Hu, Xiaohua Wu, Ying Wang, Zhongfu Lu & Pei Xu

  2. State Key Laboratory Breeding Base for Sustainable Control of Plant Pest and Disease, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People’s Republic of China

    Guojing Li & Pei Xu

Authors
  1. Xinyi Wu
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  2. Guojing Li
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  4. Yaowen Hu
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  7. Zhongfu Lu
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  8. Pei Xu
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Correspondence to Pei Xu.

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The experiments comply with the current laws of China.

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On behalf of all authors, the corresponding author states that there is no conflict of interest.

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Communicated by Matthew N Nelson.

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Wu, X., Li, G., Wang, B. et al. Fine mapping Ruv2, a new rust resistance gene in cowpea (Vigna unguiculata), to a 193-kb region enriched with NBS-type genes. Theor Appl Genet 131, 2709–2718 (2018). https://doi.org/10.1007/s00122-018-3185-4

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