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Genetic mapping of a barley leaf rust resistance gene Rph26 introgressed from Hordeum bulbosum

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The quantitative barley leaf rust resistance gene, Rph26, was fine mapped within a H. bulbosum introgression on barley chromosome 1HL. This provides the tools for pyramiding with other resistance genes.

Abstract

A novel quantitative resistance gene, Rph26, effective against barley leaf rust (Puccinia hordei) was introgressed from Hordeum bulbosum into the barley (Hordeum vulgare) cultivar ‘Emir’. The effect of Rph26 was to reduce the observed symptoms of leaf rust infection (uredinium number and infection type). In addition, this resistance also increased the fungal latency period and reduced the fungal biomass within infected leaves. The resulting introgression line 200A12, containing Rph26, was backcrossed to its barley parental cultivar ‘Emir’ to create an F2 population focused on detecting interspecific recombination within the introgressed segment. A total of 1368 individuals from this F2 population were genotyped with flanking markers at either end of the 1HL introgression, resulting in the identification of 19 genotypes, which had undergone interspecific recombination within the original introgression. F3 seeds that were homozygous for the introgressions of reduced size were selected from each F2 recombinant and were used for subsequent genotyping and phenotyping. Rph26 was genetically mapped to the proximal end of the introgressed segment located at the distal end of chromosome 1HL. Molecular markers closely linked to Rph26 were identified and will enable this disease resistance gene to be combined with other sources of quantitative resistance to maximize the effectiveness and durability of leaf rust resistance in barley breeding. Heterozygous genotypes containing a single copy of Rph26 had an intermediate phenotype when compared with the homozygous resistant and susceptible genotypes, indicating an incompletely dominant inheritance.

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Acknowledgements

The authors would like to thank Merle Forbes and Rachael Warren for technical support. Funding provided by The New Zealand Institute for Plant and Food Research Limited Strategic Science Investment Fund (Cropping Systems). Thanks to Dr Samantha Baldwin, Jamie Macalister and Dr Sathiyamoorthy Meiyalaghan for critical reading of this manuscript. Many thanks to Donna Gibson for taking the photographs and helping with the figure and table designs.

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Authors and Affiliations

  1. Department of Pest-management and Conservation, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, Canterbury, 7608, New Zealand

    Xiaohui Yu, Seona Casonato & E. Eirian Jones

  2. The New Zealand Institute for Plant & Food Research Limited, Lincoln, Canterbury, 7608, New Zealand

    Hoi Yee Kong, Vijitha Meiyalaghan, Soonie Chng, Ruth C. Butler, Richard Pickering & Paul A. Johnston

  3. Department of Wine, Food and Molecular Biosciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln, Canterbury, 7608, New Zealand

    Hoi Yee Kong

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  1. Xiaohui Yu
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  2. Hoi Yee Kong
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Correspondence to Paul A. Johnston.

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Communicated by Xiaoquan Qi.

Richard Pickering: Retired, formerly of the New Zealand Institute for Plant & Food Research Limited, Lincoln 7608, Canterbury, New Zealand.

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Yu, X., Kong, H.Y., Meiyalaghan, V. et al. Genetic mapping of a barley leaf rust resistance gene Rph26 introgressed from Hordeum bulbosum. Theor Appl Genet 131, 2567–2580 (2018). https://doi.org/10.1007/s00122-018-3173-8

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