Abstract
Key message
Two novel QTLs conferring aphid resistance were mapped and validated on soybean chromosomes 8 and 16, respectively. Closely linked markers were developed to assist breeding for aphid resistance.
Abstract
Soybean aphid, Aphis glycines Matsumura, is a highly destructive pest for soybean production. E08934, a soybean advanced breeding line derived from the wild soybean Glycine soja 85-32, has shown strong resistance to aphids. To dissect the genetic basis of aphid resistance in E08934, a mapping population (070020) consisting of 140 F3-derived lines was developed by crossing E08934 with an aphid-susceptible line E00003. This mapping population was evaluated for aphid resistance in a greenhouse trial in 2010 and three field trials in 2009, 2010, and 2011, respectively. The broad-sense heritability across the field trials was 0.84. In the mapping population 070020, two major quantitative trait loci (QTL) were detected as significantly associated with aphid resistance, and designated as Rag6 and Rag3c, respectively. Rag6 was mapped to a 10.5 centiMorgan (cM) interval between markers MSUSNP08-2 and Satt209 on chromosome 8, explaining 19.5–46.4% of the phenotypic variance in different trials. Rag3c was located at a 7.5 cM interval between markers MSUSNP16-10 and Sat_370 on chromosome 16, explaining 12.5–22.9% of the phenotypic variance in different trials. Rag3c had less resistance effect than Rag6 across all the trials. Furthermore, Rag6 and Rag3c were confirmed in two validation populations with different genetic backgrounds. No significant interaction was detected between Rag6 and Rag3c in either the mapping population or the validation populations. Both Rag6 and Rag3c were indicated as conferring antibiosis resistance to aphids by a no-choice test. The new aphid-resistance gene(s) derived from the wild germplasm G. soja 85-32 are valuable in improving soybeans for aphid resistance.
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Acknowledgements
This work was supported by United Soybean Board and Michigan Soybean Promotion Committee. We thank Paul Collins, Zixiang Wen, Feng Lin, Shair Wani, Ruijuan Tan and Sean Biehn for critical review of this manuscript.
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122_2017_2935_MOESM3_ESM.tif
Supplementary Fig. 1 Average damage indices (DI, %) of four indicator lines and two parental lines of the mapping population 070020 in a 2009 field trial; b 2010 field trial; c 2011 field trial. Bars with the same letter are not significantly different at P < 0.05 (TIFF 82 kb)
122_2017_2935_MOESM4_ESM.tif
Supplementary Fig. 2 Phenotypic distribution of soybean aphid rating score of individual plants from the validation population 110193 (a-b) and the validation population 110201(c-d). Average rating scores of parents are indicated by arrows. a 3-week rating of population 110193; b 4-week rating of population 110193; c 3-week rating of population 110201; d 4-week rating of population 110201 (TIFF 72 kb)
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Zhang, S., Zhang, Z., Bales, C. et al. Mapping novel aphid resistance QTL from wild soybean, Glycine soja 85-32. Theor Appl Genet 130, 1941–1952 (2017). https://doi.org/10.1007/s00122-017-2935-z
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DOI: https://doi.org/10.1007/s00122-017-2935-z