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Mapping soybean aphid resistance genes in PI 567598B

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Abstract

The soybean aphid (Aphis glycines Matsumura) has been a major pest of soybean [Glycine max (L.) Merr.] in North America since it was first reported in 2000. Our previous study revealed that the strong aphid resistance of plant introduction (PI) 567598B was controlled by two recessive genes. The objective of this study was to locate these two genes on the soybean genetic linkage map using molecular markers. A mapping population of 282 F4:5 lines derived from IA2070 × E06902 was evaluated for aphid resistance in a field trial in 2009 and a greenhouse trial in 2010. Two quantitative trait loci (QTLs) were identified using the composite and multiple interval mapping methods, and were mapped on chromosomes 7 (linkage group M) and 16 (linkage group J), respectively. E06902, a parent derived from PI 567598B, conferred resistance at both loci. In the 2010 greenhouse trial, each of the two QTLs explained over 30 % of the phenotypic variation. Significant epistatic interaction was also found between these two QTLs. However, in the 2009 field trial, only the QTL on chromosome 16 was found and it explained 56.1 % of the phenotypic variation. These two QTLs and their interaction were confirmed with another population consisting of 94 F2:5 lines in the 2008 and 2009 greenhouse trials. For both trials in the alternative population, these two loci explained about 50 and 80.4 % of the total phenotypic variation, respectively. Our study shows that soybean aphid isolate used in the 2009 field trial defeated the QTL found on chromosome 7. Presence of the QTL on chromosome 16 conferred soybean aphid resistance in all trials. The markers linked to the aphid-resistant QTLs in PI 567598B or its derived lines can be used in marker-assisted breeding for aphid resistance.

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Authors and Affiliations

  1. Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, 48824, USA

    Carmille Bales, Guorong Zhang, Menghan Liu, Cuihua Gu & Dechun Wang

  2. Agricultural Research Center-Hays, Kansas State University, Hays, KS, 67601, USA

    Guorong Zhang

  3. Monsanto Company, 1551 Highway 210, Huxley, IA, 50124, USA

    Clarice Mensah

  4. USDA-ARS, Soybean Genomic Laboratory, Beltsville, MD, USA

    Qijian Song, David Hyten & Perry Cregan

Authors
  1. Carmille Bales
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  2. Guorong Zhang
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  3. Menghan Liu
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  4. Clarice Mensah
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  5. Cuihua Gu
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  6. Qijian Song
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  7. David Hyten
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  8. Perry Cregan
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  9. Dechun Wang
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Corresponding author

Correspondence to Dechun Wang.

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Communicated by I. Rajcan.

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Bales, C., Zhang, G., Liu, M. et al. Mapping soybean aphid resistance genes in PI 567598B. Theor Appl Genet 126, 2081–2091 (2013). https://doi.org/10.1007/s00122-013-2120-y

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  • DOI: https://doi.org/10.1007/s00122-013-2120-y

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