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Integrated analysis of leaf morphological and color traits in different populations of Chinese cabbage (Brassica rapa ssp. pekinensis)

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QTLs and candidate gene markers associated with leaf morphological and color traits were identified in two immortalized populations of Brassica rapa, which will provide genetic information for marker-assisted breeding.

Abstract

Brassica rapa is an important leafy vegetable consumed worldwide and morphology is a key character for its breeding. To enhance genetic control, quantitative trait loci (QTLs) for leaf color and plant architecture were identified using two immortalized populations with replications of 2 and 4 years. Overall, 158 and 80 QTLs associated with 23 and 14 traits were detected in the DH and RIL populations, respectively. Among them, 23 common robust-QTLs belonging to 12 traits were detected in common loci over the replications. Through comparative analysis, five crucifer genetic blocks corresponding to morphology trait (R, J&U, F and E) and color trait (F, E) were identified in three major linkage groups (A2, A3 and A7). These might be key conserved genomic regions involved with the respective traits. Through synteny analysis with Arabidopsis, 64 candidate genes involved in chlorophyll biosynthesis, cell proliferation and elongation were co-localized within QTL intervals. Among them, SCO3, ABI3, FLU, HCF153, HEMB1, CAB3 were mapped within QTLs for leaf color; and CYCD3;1, CYCB2;4, AN3, ULT1 and ANT were co-localized in QTL regions for leaf size. These robust QTLs and their candidate genes provide useful information for further research into leaf architecture with crop breeding.

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Acknowledgments

This research was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Golden Seed Project, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (213006-05-1-SB110).

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  1. Su Ryun Choi and Xiaona Yu contributed equally to this work.

Authors and Affiliations

  1. Department of Horticulture, Chungnam National University, Daejeon, Korea

    Su Ryun Choi, Xiaona Yu, Vignesh Dhandapani, Seo Yeon Lee, Sang Heon Oh & Yong Pyo Lim

  2. Department of Horticulture, Shenyang Agricultural University, Shenyang, China

    Xiaonan Li, Wenxing Pang & Zhongyun Piao

  3. Institute of Soil and Water Conservation, Northwest A&F University, Yangling, China

    Zhi Wang

  4. School of Life Sciences, Jawaharlal Nehru University, New Delhi, India

    Nirala Ramchiary

  5. Theragen Bio Institute, TheragenEtex, Suwon, Korea

    Chang Pyo Hong

  6. Vegetable Research Division, National Institute of Horticultural and Herbal Science, Wanju-gun, Jeollabuk-do, Korea

    Suhyoung Park

  7. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea

    HyeRan Kim

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  1. Su Ryun Choi
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  2. Xiaona Yu
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Correspondence to Yong Pyo Lim.

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Communicated by Lixi Jiang.

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Choi, S.R., Yu, X., Dhandapani, V. et al. Integrated analysis of leaf morphological and color traits in different populations of Chinese cabbage (Brassica rapa ssp. pekinensis). Theor Appl Genet 130, 1617–1634 (2017). https://doi.org/10.1007/s00122-017-2914-4

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