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The first genetic map of a synthesized allohexaploid Brassica with A, B and C genomes based on simple sequence repeat markers

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We present the first genetic map of an allohexaploid Brassica species, based on segregating microsatellite markers in a doubled haploid mapping population generated from a hybrid between two hexaploid parents.

Abstract

This study reports the first genetic map of trigenomic Brassica. A doubled haploid mapping population consisting of 189 lines was obtained via microspore culture from a hybrid H16-1 derived from a cross between two allohexaploid Brassica lines (7H170-1 and Y54-2). Simple sequence repeat primer pairs specific to the A genome (107), B genome (44) and C genome (109) were used to construct a genetic linkage map of the population. Twenty-seven linkage groups were resolved from 274 polymorphic loci on the A genome (109), B genome (49) and C genome (116) covering a total genetic distance of 3178.8 cM with an average distance between markers of 11.60 cM. This is the first genetic framework map for the artificially synthesized Brassica allohexaploids. The linkage groups represent the expected complement of chromosomes in the A, B and C genomes from the original diploid and tetraploid parents. This framework linkage map will be valuable for QTL analysis and future genetic improvement of a new allohexaploid Brassica species, and in improving our understanding of the genetic control of meiosis in new polyploids.

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Acknowledgments

This work was supported partly by the Science and Technology Department of Zhejiang Province (2012C12902-1, 2011R50026-5), the Jiangsu Collaborative Innovation Center for Modern Crop Production, the Australia–China Special Fund, jointly managed by the Department of Innovation Industry Science and Research (Australia) and the Ministry of Science and Technology, and the National Natural Science Foundation (China).

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Author notes

  1. X. X. Geng

    Present address: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China

Authors and Affiliations

  1. Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou, 310058, China

    S. Yang, X. X. Geng & W. J. Zhou

  2. School of Plant Biology, The University of Western Australia, Perth, WA, 6009, Australia

    S. Chen & G. Yan

  3. The UWA Institute of Agriculture, The University of Western Australia, Perth, WA, 6009, Australia

    S. Yang, S. Chen, X. X. Geng, G. Yan & W. A. Cowling

  4. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    Z. Y. Li & J. L. Meng

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  1. S. Yang
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Correspondence to W. A. Cowling or W. J. Zhou.

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We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.

Additional information

Communicated by C. F. Quiros.

S. Yang and S. Chen are equal first authors.

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Yang, S., Chen, S., Geng, X.X. et al. The first genetic map of a synthesized allohexaploid Brassica with A, B and C genomes based on simple sequence repeat markers. Theor Appl Genet 129, 689–701 (2016). https://doi.org/10.1007/s00122-015-2657-z

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  • DOI: https://doi.org/10.1007/s00122-015-2657-z

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