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Characterization and mapping of LanrBo: a locus conferring anthracnose resistance in narrow-leafed lupin (Lupinus angustifolius L.)

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Abstract

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A novel and highly effective source of anthracnose resistance in narrow-leafed lupin was identified. Resistance was shown to be governed by a single dominant locus. Molecular markers have been developed, which can be used for selecting resistant genotypes in lupin breeding.

Abstract

A screening for anthracnose resistance of a set of plant genetic resources of narrow-leafed lupin (Lupinus angustifolius L.) identified the breeding line Bo7212 as being highly resistant to anthracnose (Colletotrichum lupini). Segregation analysis indicated that the resistance of Bo7212 is inherited by a single dominant locus. The corresponding resistance gene was given the designation LanrBo. Previously published molecular anchor markers allowed us to locate LanrBo on linkage group NLL-11 of narrow-leafed lupin. Using information from RNAseq data obtained with inoculated resistant vs. susceptible lupin entries as well as EST-sequence information from the model genome Lotus japonicus, additional SNP and EST markers linked to LanrBo were derived. A bracket of two LanrBo-flanking markers allows for precise marker-assisted selection of the novel resistance gene in narrow-leafed lupin breeding programs.

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Acknowledgments

This work was funded by the German Federal Ministry of Food and Agriculture (BMEL) within the program to promote innovation (FKZ 28-1-41.028-06) and supported by grants from the Grains Research and Development Corporation (GRDC) to KBS (UWA00147) and MN (UWA00151).

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Authors and Affiliations

  1. Julius Kühn-Institut, Institute for Breeding Research on Agricultural Crops, Groß Lüsewitz, Germany

    Kristin Fischer, Peter Wehling & Brigitte Ruge-Wehling

  2. Saatzucht Steinach GmbH & Co KG, Bocksee, Germany

    Regine Dieterich

  3. School of Plant Biology, The University of Western Australia, Crawley, Australia

    Matthew N. Nelson

  4. The UWA Institute of Agriculture, The University of Western Australia, Crawley, Australia

    Matthew N. Nelson, Lars G. Kamphuis & Karam B. Singh

  5. CSIRO, Agriculture Flagship, Wembley, Australia

    Lars G. Kamphuis & Karam B. Singh

  6. GenXPro GmbH, Frankfurt/Main, Germany

    Björn Rotter, Nicolas Krezdorn & Peter Winter

Authors
  1. Kristin Fischer
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  2. Regine Dieterich
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  3. Matthew N. Nelson
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  4. Lars G. Kamphuis
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  5. Karam B. Singh
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  6. Björn Rotter
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  7. Nicolas Krezdorn
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  8. Peter Winter
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  9. Peter Wehling
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  10. Brigitte Ruge-Wehling
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Correspondence to Kristin Fischer.

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Communicated by D. A. Lightfoot.

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Fischer, K., Dieterich, R., Nelson, M.N. et al. Characterization and mapping of LanrBo: a locus conferring anthracnose resistance in narrow-leafed lupin (Lupinus angustifolius L.). Theor Appl Genet 128, 2121–2130 (2015). https://doi.org/10.1007/s00122-015-2572-3

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  • DOI: https://doi.org/10.1007/s00122-015-2572-3

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