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SSR marker-assisted improvement of fiber qualities in Gossypium hirsutum using G. barbadense introgression lines

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Abstract

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This study demonstrates the first practical use of CSILs for the transfer of fiber quality QTLs into Upland cotton cultivars using SSR markers without detrimentally affecting desirable agronomic characteristics.

Abstract

Gossypium hirsutum is characterized by its high lint production and medium fiber quality compared to extra-long staple cotton G. barbadense. Transferring valuable traits or genes from G. barbadense into G. hirsutum is a promising but challenging approach through a traditional interspecific introgression strategy. We developed one set of chromosome segment introgression lines (CSILs), where TM-1, the genetic standard in G. hirsutum, was used as the recipient parent and the long staple cotton G. barbadense cv. Hai7124 was used as the donor parent by molecular marker-assisted selection (MAS). Among them, four CSILs, IL040-A4-1, IL080-D6-1, IL088-A7-3 and IL019-A2-6, found to be associated with superior fiber qualities including fiber length, strength and fineness QTL in Xinjiang were selected and backcrossed, and transferred these QTLs into three commercial Upland cotton cultivars such as Xinluzao (XLZ) 26, 41 and 42 grown in Xinjiang. By backcrossing and self-pollinating twice, five improved lines (3262-4, 3389-2, 3326-3, 3380-4 and 3426-5) were developed by MAS of background and introgressed segments. In diverse field trials, these QTLs consistently and significantly offered additive effects on the target phenotype. Furthermore, we also pyramided two segments from different CSILs (IL080-D6-1 and IL019-A2-6) into cultivar 0768 to accelerate breeding process purposefully with MAS. The improved lines pyramided by these two introgressed segments showed significant additive epistatic effects in four separate field trials. No significant alteration in yield components was observed in these modified lines. In summary, we first report that these CSILs have great potential to improve fiber qualities in Upland cotton MAS breeding programs.

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Abbreviations

RIL:

Recombinant inbred line

DH:

Doubled haploid

SSR:

Simple sequence repeat

QTL:

Quantitative trait locus/loci

CSIL:

Chromosome segment introgression line

MAB:

Marker-assisted backcross

BC:

Backcross

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Acknowledgments

This study was financially supported in part by grants from the Major State Basic Research Development Program of China (973 Program) (2011CB109300) and the National High Technology Research and Development Program of China (863 Program) (2011AA10A102), Philosophy Doctoral Fund Program of Xinjiang BingTuan Group (2010JC01), the Priority Academic Program Development of Jiangsu Higher Education Institutions and 111 program.

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Zhibin Cao, Peng Wang, Xiefei Zhu & Tianzhen Zhang

  2. Cotton Research Institute, Xinjiang Academy of Agriculture and Reclamation Sciences, Xinjiang, 832000, China

    Hong Chen

Authors
  1. Zhibin Cao
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  2. Peng Wang
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  3. Xiefei Zhu
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  4. Hong Chen
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  5. Tianzhen Zhang
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Corresponding author

Correspondence to Tianzhen Zhang.

Additional information

Communicated by M. Gore.

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Cao, Z., Wang, P., Zhu, X. et al. SSR marker-assisted improvement of fiber qualities in Gossypium hirsutum using G. barbadense introgression lines. Theor Appl Genet 127, 587–594 (2014). https://doi.org/10.1007/s00122-013-2241-3

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