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High-density genetic maps for loci involved in nuclear male sterility (NMS1) and sporophytic self-incompatibility (S-locus) in chicory (Cichorium intybus L., Asteraceae)

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Abstract

High-density genetic maps were constructed for loci involved in nuclear male sterility (NMS1-locus) and sporophytic self-incompatibility (S-locus) in chicory (Cichorium intybus L.). The mapping population consisted of 389 F1′ individuals derived from a cross between two plants, K28 (male-sterile) and K59 (pollen-fertile), both heterozygous at the S-locus. This F1′ mapping population segregated for both male sterility (MS) and strong self-incompatibility (SI) phenotypes. Phenotyping F1′ individuals for MS allowed us to map the NMS1-locus to linkage group (LG) 5, while controlled diallel and factorial crosses to identify compatible/incompatible phenotypes mapped the S-locus to LG2. To increase the density of markers around these loci, bulked segregant analysis was used. Bulks and parental plants K28 and K59 were screened using amplified fragment length polymorphism (AFLP) analysis, with a complete set of 256 primer combinations of EcoRI-ANN and MseI-CNN. A total of 31,000 fragments were generated, of which 2,350 showed polymorphism between K59 and K28. Thirteen AFLP markers were identified close to the NMS1-locus and six in the vicinity of the S-locus. From these AFLP markers, eight were transformed into sequence-characterized amplified region (SCAR) markers and of these five showed co-dominant polymorphism. The chromosomal regions containing the NMS1-locus and the S-locus were each confined to a region of 0.8 cM. In addition, we mapped genes encoding proteins similar to S-receptor kinase, the female determinant of sporophytic SI in the Brasicaceae, and also markers in the vicinity of the putative S-locus of sunflower, but none of these genes or markers mapped close to the chicory S-locus.

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Acknowledgments

The authors thank Bruno Desprez (Florimond Desprez Veuve et Fils, Cappelle-en-Pévèle, France) for providing seeds of the Koospol population from which the plants analyzed were derived, our colleague Bruno Delbreil for assisting in the selection of the K28 and K59 genotypes, Angélique Bourceaux, Cédric Glorieux, Eric Schmitt and Nathalie Faure, from the ‘Plateforme de culture en environnement contrôlé’, for taking care of the plants, and Jules Beekwilder, Wageningen University and Research Centre, The Netherlands, for access to the sequences of the chicory root transcriptome. Sébastien Carrère, Jerome Gouzy and Patrick Vincourt (Laboratoire Interactions Plantes Micro-organismes, INRA, Toulouse) are thanked for the construction of the Cichorium sp. database, accessible through the HeliaGene portal. We also thank Simon Hiscock, University of Bristol, UK, for his comments and contributions to improving the manuscript. Lucy Gonthier was supported by a doctoral fellowship from the Ministère de l’Enseignement Supérieur et de la Recherche, France. The project was financed by the Plant TEQ6 program (CPER PO 2007–2013), Région Nord-Pas de Calais, France and by the program CARTOCHIC (FCE OXYCHIC, SIAC 8862, 2007–2011).

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  1. Lucy Gonthier

    Present address: Université Lille 1, UMR CNRS 8198, Génétique et Evolution des Populations Végétales (GEPV), 59655, Villeneuve d’Ascq, France

Authors and Affiliations

  1. Université de Lille, UMR INRA-Lille 1 1281, Stress Abiotiques et Différenciation des Végétaux Cultivés (SADV), 59655, Villeneuve d’Ascq, France

    Lucy Gonthier, Christelle Blassiau, Monika Mörchen, Thierry Cadalen, Matthieu Poiret, Theo Hendriks & Marie-Christine Quillet

  2. Université Lille 1, GIS CARTOCHIC, UMR INRA-Lille 1 1281, Stress Abiotiques et Différenciation des Végétaux Cultivés (SADV), 59655, Villeneuve d’Ascq, France

    Monika Mörchen & Thierry Cadalen

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  1. Lucy Gonthier
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Correspondence to Theo Hendriks or Marie-Christine Quillet.

Additional information

Communicated by A. Paterson.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Online Resource 1 Features and primers for SRK-like sequences (PDF 394 kb)

Online Resource 2 Features and primers for sunflower EST-derived markers (HT) (PDF 205 kb)

122_2013_2122_MOESM3_ESM.pdf

Online Resource 3 Seed-set results of diallel and factorial crosses included 33 F1′ individuals (22 H, 11 MS) and the K28 and K59 parental genotypes. Gray squares correspond to compatible crosses, white squares to incompatible crosses, and striped squares indicate reciprocal crosses with different results. Deduced phenotypes (Phen) and genotypes (Geno) of the different compatibility groups are indicated. na: results of crosses not available (PDF 442 kb)

122_2013_2122_MOESM4_ESM.pdf

Online Resource 4 Linkage maps of LG1, LG3, LG4, LG6, LG7 and LG8 with integration of SRK-like (blue) and HT (red) derived markers (PDF 216 kb)

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Gonthier, L., Blassiau, C., Mörchen, M. et al. High-density genetic maps for loci involved in nuclear male sterility (NMS1) and sporophytic self-incompatibility (S-locus) in chicory (Cichorium intybus L., Asteraceae). Theor Appl Genet 126, 2103–2121 (2013). https://doi.org/10.1007/s00122-013-2122-9

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