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Inheritance of grain polyphenol oxidase (PPO) activity in multiple wheat (Triticum aestivum L.) genetic backgrounds

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Abstract

Grain polyphenol oxidase (PPO) activity can cause discoloration of wheat (Triticum aestivum L.) food products. Five crosses (PI 117635/Antelope; Fielder/NW03681; Fielder/Antelope; NW07OR1070/Antelope; NW07OR1066/OR2050272H) were selected to study the genetic inheritance of PPO activity. STS markers, PPO18, PPO29 and STS01, were used to identify lines with putative alleles at the Ppo-A1 and Ppo-D1 loci conditioning low or high PPO activity. ANOVA showed significant genotypic effects on PPO activity (P < 0.0001) in all populations. The generations and generation × genotype effects were not significant in any population. A putative third (null) genotype at Ppo-A1 (no PCR fragments for PPO18) was discovered in NW07OR1066 and NW07OR1070 derived populations, and these had the lowest mean PPO activities. Results demonstrated that both Ppo-A1 and Ppo-D1 loci affect the kernel PPO activity, but the Ppo-A1 has the major effect. In three populations, contrary results were observed to those predicted from previous work with Ppo-D1 alleles, suggesting the markers for Ppo-D1 allele might give erroneous results in some genetic backgrounds or lineages. Results suggest that selection for low or null alleles only at Ppo-A1 might allow development of low PPO wheat cultivars.

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Author notes

  1. Somrudee Nilthong

    Present address: School of Science Mae Fah Luang University, 333 Moo 1, Tambon Tasud Muang District, Chiang Rai, 57100, Thailand

Authors and Affiliations

  1. Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE, 68583, USA

    Somrudee Nilthong & P. S. Baenziger

  2. USDA-ARS, 137 Keim, University of Nebraska, Lincoln, NE, 68583, USA

    R. A. Graybosch

Authors
  1. Somrudee Nilthong
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  2. R. A. Graybosch
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  3. P. S. Baenziger
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Correspondence to R. A. Graybosch.

Additional information

Communicated by P. Langridge.

Née Somrudee Onto.

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Nilthong, S., Graybosch, R.A. & Baenziger, P.S. Inheritance of grain polyphenol oxidase (PPO) activity in multiple wheat (Triticum aestivum L.) genetic backgrounds. Theor Appl Genet 125, 1705–1715 (2012). https://doi.org/10.1007/s00122-012-1947-y

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  • DOI: https://doi.org/10.1007/s00122-012-1947-y

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