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Characterization of interploid hybrids from crosses between Brassica juncea and B. oleracea and the production of yellow-seeded B. napus

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Abstract

Yellow-seeded Brassica napus was for the first time developed from interspecific crosses using yellow-seeded B. juncea (AABB), yellow-seeded B. oleracea (CC), and black-seeded artificial B. napus (AACC). Three different mating approaches were undertaken to eliminate B-genome chromosomes after trigenomic hexaploids (AABBCC) were generated. Hybrids (AABCC, ABCC) from crosses AABBCC × AACC, AABBCC × CC and ABCC × AACC were advanced by continuous selfing in approach 1, 2 and 3, respectively. To provide more insight into Brassica genome evolution and the cytological basis for B. napus resynthesis in each approach, B-genome chromosome pairing and segregation were intensively analyzed in AABCC and ABCC plants using genomic in situ hybridization methods. The frequencies at which B-genome chromosomes underwent autosyndesis and allosyndesis were generally higher in ABCC than in AABCC plants. The difference was statistically significant for allosyndesis but not autosyndesis. Abnormal distributions of B-genome chromosomes were encountered at anaphase I, including chromosome lagging and precocious sister centromere separation of univalents. These abnormalities were observed at a significantly higher frequency in AABCC than in ABCC plants, which resulted in more rapid B-genome chromosome elimination in the AABCC derivatives. Yellow or yellow-brown seeds were obtained in all approaches, although true-breeding yellow-seeded B. napus was developed only in approaches 2 and 3. The efficiency of the B. napus construction approaches was in the order 1 > 3 > 2 whereas this order was 3 > 2 > 1 with respect to the construction of yellow-seeded B. napus. The results are discussed in relation to Brassica genome evolution and the development and utilization of the yellow-seeded B. napus obtained here.

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Acknowledgments

This research was financed by the High-Tech program ‘‘863’’ (2011AA10A104), the Program for Modern Agricultural Industrial Technology System (nycytx-00501) and the National Natural Science Foundation of China (31000721).

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Authors and Affiliations

  1. National Key Lab of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Jing Wen, Lixia Zhu, Liping Qi, Hongmei Ke, Bin Yi, Jinxiong Shen, Jinxing Tu, Chaozhi Ma & TingDong Fu

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  1. Jing Wen
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  2. Lixia Zhu
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  3. Liping Qi
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  4. Hongmei Ke
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Correspondence to TingDong Fu.

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Communicated by I. Rajcan.

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Wen, J., Zhu, L., Qi, L. et al. Characterization of interploid hybrids from crosses between Brassica juncea and B. oleracea and the production of yellow-seeded B. napus . Theor Appl Genet 125, 19–32 (2012). https://doi.org/10.1007/s00122-012-1813-y

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