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Molecular markers show a complex mosaic pattern of wheat-Thinopyrum intermedium translocations carrying resistance to YDV

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Abstract

Thinopyrum intermedium translocations derived from the wheat (Triticum aestivum L.) substitution line P-29 were previously characterized by RFLP. We have further analyzed these lines and additional related germplasm with publicly available STS and SSRs. Primers which showed a polymorphism between wheat and P-29, were tested in all recombinant and nulli-tetrasomic lines confirming their position on chromosome 7D. The resulting 7D/7E chromosome maps appeared as a mosaic of wheat and Th. intermedium chromatin sections. To verify the composition of the translocation lines suggested by the RFLP-PCR map, F2 progeny of two crosses (CS/216-1 and CS/260-1) were analyzed with molecular markers. Both populations gave an unexpectedly diverse number of recombinant individuals, suggesting that interstitial translocations occur more frequently than previously thought. This analysis also showed that there is a wide range in the number and position of the interstitial translocations within a given line such as the mosaic chromosome in recombinant line 260-1/CS-26, which has four Th. intermedium chromosome segments. Phenotypic data of the two populations suggested the presence of one gene which we have called Bdv3 to differentiate it from the previously reported orthologous gene Bdv2. Using the PCR-based molecular markers identified in this study, 5 out of 12 elite lines that showed good yields and no YDV symptoms contained Th. intermedium chromatin. Due to the multiple components involved in the YDV disease complex, combining selection for YDV resistance with the molecular markers and maps identified in this study will increase the efficiency of introgressing Th. intermedium chromatin containing YDV resistance or other beneficial traits into elite wheat germplasm.

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Acknowledgments

The authors acknowledge financial support by USDA-ARS CRIS project 3602-21220-008-00D and SCA project 58-3602-2-152 and Purdue University Agricultural Research programs. Mention of a trademark, proprietary product, trade names or commercial products in this article is solely for the purpose of providing scientific information; it does not constitute a guarantee, warranty, recommendation, or endorsement by the USDA and does not imply approval to the exclusion of other products that also may be suitable. We are grateful to Amanda Platteter and Katie Head for their technical assistance. A big thank you, to Phil Larkin for reading the final versions of this manuscript.

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Authors and Affiliations

  1. Agronomy Department, Purdue University, 915 W State Street, West Lafayette, IN, 47907-2054, USA

    Ligia Ayala-Navarrete, H. Ohm & J. Anderson

  2. United States Department of Agriculture, Agricultural Research Service (USDA-ARS), 915 W. State Street, West Lafayette, IN, 47907-2054, USA

    N. Thompson & J. Anderson

  3. Plant Industry CSIRO Clunies Ross and Barry Drive, Canberra, 2601, Australia

    Ligia Ayala-Navarrete

  4. BSES Limited 50 Meiers Road, Indooroopilly, QLD, 4068, Australia

    N. Thompson

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  1. Ligia Ayala-Navarrete
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Correspondence to Ligia Ayala-Navarrete.

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Communicated by P. Heslop-Harrison.

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Ayala-Navarrete, L., Thompson, N., Ohm, H. et al. Molecular markers show a complex mosaic pattern of wheat-Thinopyrum intermedium translocations carrying resistance to YDV. Theor Appl Genet 121, 961–970 (2010). https://doi.org/10.1007/s00122-010-1365-y

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