Abstract
We analysed the products of male meiosis in microspore-derived progeny from a Brassica napus (AACnCn) × Brassica carinata (BBCcCc) interspecific hybrid (ABCnCc). Genotyping at 102 microsatellite marker loci and nuclear DNA contents provided strong evidence that 26 of the 28 progeny (93%) were derived from unreduced (2n) gametes. The high level of CnCc marker heterozygosity, and parallel spindles at Anaphase II in the ABCnCc hybrid, indicated that unreduced gametes were formed by first division restitution. The frequency of dyads at the tetrad stage of pollen development (2.6%) suggested that unreduced gametes were preferentially selected in microspore culture. Segregation of marker alleles in the microspore-derived progeny was consistent with homologous recombination between Cn and Cc chromosomes and homoeologous recombination involving A-, B- and C-genome chromosomes during meiosis in the ABCnCc hybrid. We discuss the potential for using microspore culture of unreduced gametes in interspecific hybrids to map Brassica centromeres through half-tetrad analysis.
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Acknowledgments
We thank Anouska Cousin (Canola Breeders Western Australia Pty Ltd) for technical guidance in microspore culture, and Dr. Paul Kron (University of Guelph, Canada) for technical guidance in flow cytometry. We thank Dr. Kathy Heel and Dr. Paul Rigby of the UWA Centre for Microscopy, Characterisation and Analysis for help in flow cytometry and pollen analysis. We also thank Dr. Anne-Marie Chèvre and Prof. Carlos Quiros for helpful suggestions in preparing this manuscript. The work was supported by the Australian Research Council Linkage Project LP0667805 with industry partners Norddeutsche Pflanzenzucht Hans-Georg Lembke KG (Germany) and the Council of Grain Grower Organisations Ltd (Australia). AEM and LT were supported by scholarships from the Cooperative Research Centre for Value Added Wheat. MNN and WAC were supported for this research by a grant from Export Grains Centre Ltd.
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Communicated by C. Quiros.
An erratum to this article can be found at http://dx.doi.org/10.1007/s00122-009-1126-y
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Nelson, M.N., Mason, A.S., Castello, MC. et al. Microspore culture preferentially selects unreduced (2n) gametes from an interspecific hybrid of Brassica napus L. × Brassica carinata Braun. Theor Appl Genet 119, 497–505 (2009). https://doi.org/10.1007/s00122-009-1056-8
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DOI: https://doi.org/10.1007/s00122-009-1056-8