Skip to main content
SpringerLink
Log in

A major QTL and an SSR marker associated with glycoalkaloid content in potato tubers from Solanum tuberosum × S. sparsipilum located on chromosome I

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

New potato (Solanum tuberosum) varieties are required to contain low levels of the toxic glycoalkaloids and a potential approach to obtain this is through marker-assisted selection (MAS). Before applying MAS it is necessary to map quantitative trait loci (QTLs) for glycoalkaloid content in potato tubers and identify markers that link tightly to this trait. In this study, tubers of a dihaploid BC1 population, originating from a cross between 90-HAF-01 (S. tuberosum 1) and 90-HAG-15 (S. tuberosum 2 × S. sparsipilum), were evaluated for content of α-solanine and α-chaconine (total glycoalkaloid, TGA) after field trials. In addition, tubers were assayed for TGA content after exposure to light. A detailed analysis of segregation patterns indicated that a major QTL is responsible for the TGA content in tubers of this potato population. One highly significant QTL was mapped to chromosome I of the HAG and the HAF parent. Quantitative trait loci for glycoalkaloid production in foliage of different Solanum species have previously been mapped to this chromosome. In the present research, QTLs for α-solanine and α-chaconine content were mapped to the same location as for TGA content. Similar results were observed for tubers exposed to light. The simple sequence repeat marker STM5136 was closely linked to the identified QTL.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Boluarte-Medina T, Fogelman E, Chani E, Miller AR, Levin I, Levy D, Veilleux RE (2002) Identification of molecular markers associated with leptine in reciprocal backcross families of diploid potato. Theor Appl Genet 105:1010–1018

    Article  CAS  Google Scholar 

  • Dale MFB, Griffiths DW, Bain H (1992) Glycoalkaloids in potatoes-shedding light on an important problem. Aspects Appl Biol 33:221–227

    Google Scholar 

  • Deahl KL, Cantelo WW, Sinden SL, Sanford LL (1991) The effect of light intensity on Colorado potato beetle resistance and foliar glycoalkaloid concentration of four Solanum chacoense clones. Am Potato J 68:659–666

    Article  CAS  Google Scholar 

  • Dellaporta SL, Wood J, Hicks B (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21

    Article  CAS  Google Scholar 

  • Dimenstein L, Lisker N, Kedar N, Levy D (1997) Changes in the content of steroidal glycoalkaloids in potato tubers grown in the field and in the greenhouse under different conditions of light, temperature and daylength. Physiol Mol Plant Pathol 50:391–402

    Article  CAS  Google Scholar 

  • Friedman M, Dao L (1992) Distribution of glycoalkaloids in potato plants and commercial potato products. J Agric Food Chem 40:419–423

    Article  CAS  Google Scholar 

  • Friedman M, McDonald GM (1997) Potato glycoalkaloids: chemistry, analysis, safety, and plant physiology. Crit Rev Plant Sci 16:55–132

    Article  CAS  Google Scholar 

  • Friedman M, Henika PR, Mackey BE (2003) Effect of feeding solanidine, solasodine and tomatidine to non-pregnant and pregnant mice. Food Chem Toxicol 41:61–71

    Article  PubMed  CAS  Google Scholar 

  • Friedman M, Lee K-R, Kim HJ, Lee I-S, Kozukue N (2005) Anticarcinogenic effects of glycoalkaloids from potatoes against human cervical, liver, lymphoma, and stomach cancer cells. J Agric Food Chem 53:6162–6169

    Article  PubMed  CAS  Google Scholar 

  • Gebhardt C, Ritter E, Barone A, Debener T, Walkemeier B, Schachtschabel U, Kaufmann H, Thompson RD, Bonierbale MW, Ganal MW, Tanksley SD, Salamini R (1991) RFLP maps of potato and their alignment with the homoeologous tomato genome. Theor Appl Genet 83:49–57

    Article  Google Scholar 

  • Griffiths DW, Dale MFB (2001) Effect of light exposure on the glycoalkaloid content of Solanum phureja tubers. J Agric Food Chem 49:5223–5227

    Article  PubMed  CAS  Google Scholar 

  • Hellenäs K-E, Branzell C (1997) Liquid chromatographic determination of the glycoalkaloids α-solanine and α-chaconine in potato tubers: NMKL interlaboratory study. J AOAC Int 80:549–554

    PubMed  Google Scholar 

  • Hellenäs K-E, Branzell C, Johnsson H, Slanina P (1995) High levels of glycoalkaloids in the established Swedish potato variety Magnum Bonum. J Sci Food Agric 68:249–155

    Article  Google Scholar 

  • Hlywka JJ, Stephenson GR, Sears MK, Yada RY (1994) Effects of insect damage on glycoalkaloid content in potatoes (Solanum tuberosum). J Agric Food Chem 42:2545–2550

    Article  CAS  Google Scholar 

  • Hutvágner G, Bánfalvi Z, Milánkovics I, Silhavy D, Polgár Z, Horváth S, Wolters P, Nap J-P (2001) Molecular markers associated with leptinine production are located on chromosome 1 in Solanum chacoense. Theor Appl Genet 102:1065–1071

    Article  Google Scholar 

  • Jonasson T, Olsson K (1994) The influence of glycoalkaloids, chlorogenic acid and sugars on the susceptibility of potato tubers to wireworm. Potato Res 37:205–216

    Article  CAS  Google Scholar 

  • Jørgensen B, Labouriau R, Lundbye-Christensen S (1996) Linear Growth curve analysis based on exponential dispersion models. J R Stat Soc B 58:573–592

    Google Scholar 

  • Milbourne D, Meyer RC, Collins AJ, Ramsay LD, Gebhardt C, Waugh R (1998) Isolation, characterisation and mapping of simple sequence repeat loci in potato. Mol Gen Genet 259:233–245

    Article  PubMed  CAS  Google Scholar 

  • Morris SC, Lee TH (1984) The toxicity and teratogenicity of Solanaceae glycoalkaloids, particularly those of the potato (Solanum tuberosum): a review. Food Tech Aust 36:118–124

    CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:493–497

    Article  Google Scholar 

  • Olsson K (1986) The influence of genotype on the effects of impact damage on the accumulation of glycoalkaloids in potato tubers. Potato Res 29:1–12

    Article  CAS  Google Scholar 

  • Osman SF, Herb SF, Fitzpatrick TJ, Schmiediche P (1978) Glycoalkaloid composition of wild and cultivated tuber-bearing Solanum species of potential value in potato breeding programs. J Agric Food Chem 26:1246–1248

    Article  CAS  Google Scholar 

  • Osman SF, Sinden SL, Deahl KL, Moreau R (1987) Metabolism of solanidine by microsomal fractions from Solanum chacoense. Phytochemistry 26:3163–3165

    Article  CAS  Google Scholar 

  • Provan J, Powell W, Waugh R (1996) Microsatellite analysis of relationships within cultivated potato (Solanum tuberosum). Theor Appl Genet 92:1078–1084

    Article  CAS  Google Scholar 

  • Ramsay G, Griffiths DW, Deighton N (2004) Patterns of solanidine glycoalkaloid variation in four gene pools of the cultivated potato. Genet Res Crop Evol 2004:805–813

  • Ronning CM, Sanford LL, Kobayashi RS, Kowalski SP (1998) Foliar leptine production in segregating F1, Inter-F1, and backcross families of Solanum chacoense Bitter. Am J Potato Res 75:137–143

    CAS  Google Scholar 

  • Ronning CM, Stommel JR, Kowalski SP, Sanford LL, Kobayashi RS, Pineada O (1999) Identification of molecular markers associated with leptine production in a population of Solanum chacoense Bitter. Theor Appl Genet 98:39–46

    Article  CAS  Google Scholar 

  • Sagredo B, Lafta A, Casper H, Lorenzen J (2006) Mapping of genes associated with leptine content of tetraploid potato. Theor Appl Genet 114:131–142

    Article  PubMed  CAS  Google Scholar 

  • Sanford LL, Deahl KL, Sinden SL, Ladd TL Jr (1992) Glycoalkaloid contents in tubers from Solanum tuberosum populations selected for potato leafhopper resistance. Am Potato J 69:693–703

    Article  CAS  Google Scholar 

  • Sanford LL, Kobayashi RS, Deahl KL, Sinden SL (1996) Segregation of leptines and other glycoalkaloids in Solanum tuberosum (4×) × S. chacoense (4×) crosses. Am Potato J 73:21–33

    Article  CAS  Google Scholar 

  • Sinden SL, Sanford LL, Webb RE (1984) Genetic and environmental control of potato glycoalkaloids. Am Potato J 61:141–156

    Article  CAS  Google Scholar 

  • Smith DB, Roddick JG, Jones JL (1996) Potato glycoalkaloids: Some unanswered questions. Trends Food Sci Tech 7:126–131

    Article  CAS  Google Scholar 

  • Sørensen KK, Madsen MH, Kirk HG, Madsen DK, Torp AM (2006) Linkage and QTL mapping of foliage late blight resistance in the wild species Solanum vernei. Plant Breed 125:268–276

    Article  Google Scholar 

  • Thorne HV, Clarke GF, Skuce R (1985) The inactivation of Herpes simplex virus by some Solanaceae glycoalkaloids. Antiviral Res 5:335–343

    Article  PubMed  CAS  Google Scholar 

  • Van Dam J, Levin I, Struik PC, Levy D (1999) Genetic characterisation of tetraploid potato (Solanum tuberosum L.) emphasising genetic control of total glycoalkaloid content in the tubers. Euphytica 110:67–76

    Article  Google Scholar 

  • Van Dam J, Levin I, Struik PC, Levy D (2003) Identification of epistatic interaction affecting glycoalkaloid content in tubers of tetraploid potato (Solanum tuberosum L.). Euphytica 134:353–360

    Article  Google Scholar 

  • Van Gelder WMJ (1991) Chemistry, toxicology, and occurrence of steroidal glycoalkaloids: potential contaminants of the potato (Solanum tuberosum L.). In: Rizk AF (ed) Poisonous plant contamination of edible plants. CRC Press, Boca Raton, pp 117–156

    Google Scholar 

  • Van Gelder WMJ, Vinke JH, Scheffer JJC (1988) Steroidal glycoalkaloids in tubers and leaves of Solanum species used in potato breeding. Euphytica 39S:147–158

    Google Scholar 

  • Van Gelder WMJ, Tuinstra LGMT, van der Greef J, Scheffer JJC (1989) Characterization of novel steroidal alkaloids from tubers of Solanum species by combined gas chromatography–mass spectrometry. Implications for potato breeding. J Chromatogr 482:13–22

    Article  Google Scholar 

  • Van Ooijen JW, Voorrips RE (2001) JoinMap® 3.0 Software for the calculation of genetic linkage maps. Plant Research International, Wageningen

    Google Scholar 

  • Van Ooijen JW, Boer MP, Jansen RC, Maliepaard C (2002) MapQTL® 4.0 Software for the calculation of QTL positions on genetic maps. Plant Research International, Wageningen

    Google Scholar 

  • Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Heredity 93:77–78

    Article  CAS  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  • Yencho GC, Kowalski SP, Kobayashi RS, Sinden SL, Bonierbale MW, Deahl KL (1998) QTL mapping of foliar glycoalkaloid aglycones in Solanum tuberosum × S. berthaultii potato progenies: quantitative variation and plant secondary metabolism. Theor Appl Genet 97:563–574

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was funded mainly by The Directorate for Food, Fisheries and Agri Business and partially by The Potato Levy Foundation, Denmark. The authors thank Mrs. Rita Svensson and Mrs. Eva Nordqvist, SW Laboratory, Svalöf Weibull AB, Sweden, for helping us with the glycoalkaloid analyses and Dr. Louise Bach Jensen, Department of Genetics and Biotechnology, University of Aarhus, Denmark, for helping us to carry out MapQTL analyses.

Author information

Author notes

  1. Kerstin Olsson

    Present address: Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Box 44, 230 53, Alnarp, Sweden

Authors and Affiliations

  1. Department of Agroecology and Environment, Faculty of Agricultural Sciences, University of Aarhus, P·O. Box 50, 8830, Tjele, Denmark

    Kirsten Kørup Sørensen & Jørgen Christiansen

  2. Danish Potato Breeding Foundation, Grindstedvej 55, 7184, Vandel, Denmark

    Hanne Grethe Kirk

  3. SW Laboratory, Svalöf Weibull AB, 268 81, Svalöv, Sweden

    Kerstin Olsson

  4. Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, P·O. Box 50, 8830, Tjele, Denmark

    Kirsten Kørup Sørensen & Rodrigo Labouriau

Authors
  1. Kirsten Kørup Sørensen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hanne Grethe Kirk
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kerstin Olsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rodrigo Labouriau
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jørgen Christiansen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kirsten Kørup Sørensen.

Additional information

Communicated by R. Waugh.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sørensen, K.K., Kirk, H.G., Olsson, K. et al. A major QTL and an SSR marker associated with glycoalkaloid content in potato tubers from Solanum tuberosum × S. sparsipilum located on chromosome I. Theor Appl Genet 117, 1–9 (2008). https://doi.org/10.1007/s00122-008-0745-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-008-0745-z

Keywords

Search

Navigation