Abstract
Amphiphilic proteomic analysis was carried out on the ITMI (International Triticae Mapping Population) population resulting from a cross between "Synthetic", i.e.: "W7984" and "Opata". Out of a total of 446 spots, 170 were specific to either of the two parents, and 276 were common to both. Preliminary analysis, which was performed on 80 progenies (Amiour et al. 2002a), was completed here using a total of 101 selfed lines. Seventy two Loci of amphiphilic spots placed at LOD = 5 were conclusively assigned to15 chromosomes. Some spots mapped during the first analysis were eliminated because of the significant distortion segregation observed in the second analysis. Group-1 chromosomes had by far the greatest number of mapped spots (51). Using the Quantitative Trait Loci (QTLs) approach, analysis of the quantitative variation of each spot revealed that 96 spots out of the 170 specific ones showed at least one Protein Quantity Locus (PQL). These PQLs were distributed throughout the genome. With Matrix Laser Desorption Ionisation Time Of Flight (MALDI-TOF) spectrometry and Database interrogation, a total of 93 specific and 41 common spots were identified. This enabled us to show that the majority of these proteins are associated with membranes and/or play a role in plant defence against external invasions. Using multiple-regression analysis, other amphiphilic proteins, in addition to puroindolines, were shown to be involved in variation in kernel hardness in the ITMI population.
Similar content being viewed by others
References
American Association of Cereal Chemists (1995) Approved methods of the AACC, 9th ed. method 39670A. The Association, St. Paul, Minnesota
Amiour N, Merlino M, Leroy P, Branlard G (2002a) Proteomic analysis of amphiphilic proteins of hexaploïd wheat kernels. Proteomics 2:632–641
Amiour N, Jahier J, Tanguy AM, Chiron H, Branlard G (2002b) Effect of 1R(1A), 1R(1B), 1R(1D) substitution on technological value of bread wheat. J Cereal Sci 35:149–160
Branlard G, Amiour N, Igrejas G, Gaborit T, Herbette S, Dardevet M, Marion D (2003) Diversity of puroindolines as revealed by two-dimensional electrophoresis. Proteomics 3:168–174
Büttner M, Singh KB (1997) Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), and ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein. Proc Natl Acad Sci USA 94:5961–5966
Blum H, Beir H, Gross HJ (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamid gels. Electrophoresis 8:93–99
Casaretto JA, Corcuera LJ (1995) Plant proteinase inhibitors: a defensive response against insects. Biol Res 28:239–249
Doekes GJ, Belderok B (1976) Kernel hardness and baking quality of wheat—a genetic analysis using chromosome substitution lines. Euphytica 25:565–576
Dubreil L, Compoint JP, Marion D (1997) The interaction of puroindolines with wheat polar lipids determines their foaming properties. J Agric Food Chem 45:108–116
Facchini PJ, Yu M, Penzes-Yost C (1999) Decreased cell-wall digestibility in Canola transformed with chimeric tyrosine decarboxylase genes from Opium Poppy. Plant Physiol 120:653–663
Galvez S, Roche O, Bismuth E, Brown S, Gadal P, Hodges M (1998) Mitochondrial localization of a NADP-dependent (corrected) isocitrate dehydrogenase isoenzyme by using the green fluorescent protein as a marker. Proc Natl Acad Sci USA 95:7813–7818
Greenwell P, SchofieldJD (1986) A starch granule protein associated with endosperm softness in wheat. Cereal Chem 63:379–380
Hong MA, Yanofsky MF, Meyerowitz EM (1990) Molecular cloning and characterization of GPA1, a G protein α subunit gene from Arabidopsis thaliana. Proc Natl Acad Sci USA 87:3821–3825
Igrejas G, Leroy P, Charmet G, Gaborit T, Marion D, Branlard G (2002) Mapping QTLs for grain hardness and puroindoline content in wheat (Triticum aestivum L.). Theor Appl Genet 106:19–27
Jebanathirajah JA, Coleman JR (1998) Association of carbonic anhydrase with a Calvin cycle enzyme complex in Nicotiana tabacum. Planta 204:177–182
Kachroo P, Shanklui J, Shah J, Wittle EJ, Klessig DF (2001) A fatty acid desaturase modulates the activation of defense signaling pathways in plants. Proc Natl Acad Sci USA 98:9448–9453
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Lincoln S, Daly M, Lander E (1992) Mapping genes controlling quantitative traits with MAPMAKER/QTL 1.1. Whitehead Institute Technical Report, Cambridge, Massachusetts
Li WL, Faris JD, Chittoor JM, Leach JE, Hulbert SH, Liu DJ, Chen PD, Gill BS (1999) Genomic mapping of defense response genes in wheat. Theor Appl Genet 98:226–233
Marion D, Nicolas Y, Popineau Y, Branlard G, Landry J (1994) A new and improved sequential extraction procedure of wheat proteins. In: Wheat kernel proteins. Viterbo, Italie, pp 197–199
Maxwell DP, Wang Y, McIntosh L (1999) The alternative oxidase lowers mitochondrial reactive oxygen production in plant cells. Proc Natl Acad Sci USA 96:8271–8276
Mesa R, Salomon C, Roggero M, Stahl PD, Mayorga LS (2001) Rab22a affects the morphology and function of the endocytic pathway. J Cell Sci 114:4041–4049
McIntosh RA, Hart GE, Gale MD (1998) Catalogue of gene symbols for wheat. In: Slinkard AE (ed) IXth Int Wheat Genet Symposium, , August 2–7th 1998, Saskatoon, Canada, pp 1333–1500
Moons A, Bauw G, Prinsen E, Van Montagu M, Van der Straeten D (1995) Molecular and physiological responses to abscissic acid and salts in roots of salt-sensitive and salt-tolerant Indica rice varieties. Plant Physiol 107:177–186
Morris CF (2002) Puroindolines: the molecular genetic basis of wheat grain hardness. Plant Mol Biol 48:633–647
Morris CF, Greenblatt GA, Bettge AD, Malkawi HI (1994) Isolation and characterisation of multiple forms of friabilin. J Cereal Sci 21:167–174
Morrison WR, Law CN, Wylie LJ, Coventry AM, Seekings J (1989) The effect of group-5 chromosomes on the free polar lipids and breadmaking quality of wheat. J Cereal Sci 9:41–51
Richardson M (1977) The proteinase inhibitors of plants and micro-organisms. Phytochemistry 16:159–169
Sheehan D, Meade G, Foley VM, Dowd CA (2001) Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily. Biochem J 360:1–16
Sourdille P, Perretant MR, Charmet G, Leroy P, Gautier MF, Joudrier P, Nelson JC, Sorrells ME, Bernard M (1996) Linkage between RFLP markers and genes affecting kernel hardness in wheat. Theor Appl Genet 92:580–586
Tang C, Zhang W, Chait BT, Feyö D (2000) A method to evaluate the quality of databases search results, ABRF 2000, Bellevue, Washington
Taylor CA, Graveland A, Henderson MH, Kerkhoff MAT, Veerman SM (1994) Distribution of total and protein-bound glutathione in wheat flours and gluten. In: Wheat Kernel Proteins Molecular and Functional Aspects. Viterbo, Italie, pp 339–344
Turnbull KM, Gaborit T, Marion D, Rahman S (2000) Variation in puroindoline polypeptides in Australian wheat cultivars in relation to grain hardness. Aust J Plant Physiol 27:153–158
Wang X (2000) Multiple forms of phospholipase D in plants: the gene family, catalytic and regulatory properties, and cellular functions. Prog Lipid Res 39:109–49
Zhang W, Chait BT (2000) ProFound–an expert system for protein identification using mass spectrometric peptide-mapping information. Anal Chem 72:2482–2489
Acknowledgements
Celine Henry (INRA, Joy-en-Josas) and Christophe Chambon ( INRA, Clermont-Ferrand- Theix), who performed Mass Spectrometry, and Michel Beckert (INRA, Clermont Ferrand), who granted the post-doctoral activities of N. Amiour, are gratefully thanked.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by J.W. Snape
Rights and permissions
About this article
Cite this article
Amiour, N., Merlino, M., Leroy, P. et al. Chromosome mapping and identification of amphiphilic proteins of hexaploid wheat kernels. Theor Appl Genet 108, 62–72 (2003). https://doi.org/10.1007/s00122-003-1411-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00122-003-1411-0