Abstract
The South American Leaf Blight (SALB), caused by the fungus Microcyclus ulei, is the major rubber tree disease in all Central and South America. A population of 192 progeny individuals derived from a cross between a resistant clone and a susceptible cultivated clone was planted in a field trial in French Guiana in order to evaluate the resistance parameters under real infestation conditions. The resistance type (RT), the presence of stromata (ST) and the level of attack (AT) were observed 20-times on a 22-months period, and semi-quantitative evaluation of stromata was registered only once. The search for QTLs was performed using the Kruskal-Wallis test, Interval Mapping and the Composite Interval Mapping method. One major QTL located on linkage group g13 was detected on the RO 38 map, responsible for 36 to 89% of the phenotypic variance of resistance. This resistance QTL corresponds to one that had previously been detected under controlled conditions of infestation and we called it M13-1bn. Surprisingly, the effect of this QTL was larger under natural conditions of infestation than under controlled inoculation. Other minor QTLs (four on the RO38 map and one on the PB 260 map) were also detected. The type of resistance brought by M13-1bn, as well as its durability, are discussed. Applications for rubber tree breeding programs are considered.
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Cervera MT, Gusmao J, Steenackers M, Peteman J, Storme V, van den Broeck A, van Montagu M, Boerjan W (1996) Identification of AFLP molecular markers for resistance against Melampsora larici-populina in Populus. Theor Appl Genet 93:733–737
Chee KH, Holliday P (1986) South american leaf blight of Hevea rubber. MRRDB. Kuala Lumpur, Malasya
Chee KH, Darmono TW, Zhang KM, Lieberei R (1985) Leaf development and spore production and germination after infection of Hevea leaves by Microcyclus ulei. J Rubber Res Institute of Malaya 33:124–137
Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971
Darmono TW, Chee KH (1985) Reaction of hevea clones to races of Microcyclus ulei in Brazil. J Rubber Res Institute of Malaya 33:1–8
Dean W (1987) Brazil and the struggle for rubber. Cambridge University Press, UK
Donald TM, Pellerone F, Adam-Blondon A-F, Bouquet A, Thomas MR, Dry IB (2002) Identification of resistance gene analogs linked to a powdery mildew resistance locus in grapevine. Theor Appl Genet 104:610–618
Garcia D, Cazaux E, Rivano F, d'Auzac J (1995) Chemical and structural barriers to Microcyclus ulei, the agent of South American leaf blight in Hevea spp. Eur J Forest Pathol 25:282–292
Gonçalves JRC (1968) The resistance of Fx and IAN rubber clones to leaf diseases in Brazil. Trop Agric, Trinidad 45:331–336
Hashim I, Almeida LCC (1987) Identification of races and in vitro sporulation of Microcyclus ulei. J Natural Rubber Res 2:111–117
Holliday P (1970) South American Leaf Blight (Microcyclus ulei) of Hevea brasiliensis. Phytopathol Papers 12:1–30
Jansen RC (1993) Interval Mapping of multiple quantitative trait loci. Genetics 135:205–211
Jorge V, Fregene MA, Duque MC, Bonierbale MW, Tohme J, Verdier V (2000) Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz). Theor Appl Genet 101:865–872
Jorge V, Fregene MA, Vélez CM, Duque MC, Tohme J, Verdier V (2001) QTL analysis of field resistance to Xanthomonas axonopodis pv manihotis in cassava. Theor Appl Genet 102:564–571
Junqueira NTV, Chaves GM, Zambolim L, Gasparotto L, Alfenas AC (1986) Variabilidade fisiológica de Microcyclus ulei. Fitopatologia Brasileira 11:823–833
Junqueira NTV, Chaves GM, Zambolim L, Alfenas AC, Gasparotto L (1988) Reação de clones de seringueira a varios isolados de Microcyclus ulei. Pesquisa Agropecuária Brasileira 23:877–893
Junqueira NTV, Lieberei R, Kalil Filho AN, Lima MIPM (1990) Components of partial resistance in Hevea clones to rubber tree leaf blight, caused by Microcyclus ulei. Fitopatologia Brasileira 15:211–214
King GJ, Alston FH, Brown LM, Chevreau E, Evans KM, Dunemann F, Janse J, Laurens F, Lynn JR, Maliepaard C (1998) Multiple field and glasshouse assessments increase the reliability of linkage mapping of the Vf source of scab resistance in apple. Theor Appl Genet 96:699–708
Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199
Le Guen V, Seguin M, Mattos CRR (2000) Qualitative resistance of Hevea to Phyllachora huberi P. Henn. Euphytica 112:211–217
Lespinasse D (1999) Cartographie génétique de l'hévéa (Hevea spp.) et déterminisme génétique de la résistance à Microcyclus ulei, champignon pathogène de l'hévéa, PhD thesis, Université Montpellier II, France
Lespinasse D, Rodier-Goud M, Grivet L, Leconte A, Legnate H, Seguin M (2000a) A saturated genetic linkage map of rubber tree (Hevea spp.) based on RFLP, AFLP, microsatellite, and isozyme markers. Theor Appl Genet 100:127–138
Lespinasse D, Grivet L, Troispoux V, Rodier-Goud M, Pinard F, Seguin M (2000b) Identification of QTLs involved in the resistance to South American leaf blight (Microcyclus ulei) in the rubber tree. Theor Appl Genet 100:975–984
Mattos CRR, Pinard F, Le Guen V (1999) Variabilidade de isolados de Microcyclus ulei em uma plantação de seringueira (Hevea spp.) no sudeste da Bahia. XXXII Congresso Brasileiro de Fitopatologia, Curitiba (PR) Brasil, agosto 1999
Newcombe G, Bradshaw HDJ, Chastagner GA, Stettler RF (1996) A major gene for resistance to Melampsora medusae f.sp. deltoidae in a hybrid poplar pedigree. Phytopathology 86:87–94
Patocchi A, Gianfranceschi L, Gessler C (1999) Towards the map-based cloning of Vf : fine and physical mapping of the Vf region. Theor Appl Genet 99:1012–1017
Pauquet J, Bouquet A, This P, Adam-Blondon A-F (2001) Establishment of a local map of AFLP markers around the powdery mildew resistance gene Run1 in grapevine, and assessment of their usefulness for marker-assisted selection. Theor Appl Genet 103:1201–1210
Pinheiro FSV, Alves RM, Conduru Neto JMH (1984) Herança da resistência ao Microcyclus ulei e perspectivas do melhoramento genético da seringueira. IV. Seminàrio nacional da seringueira. EMBRAPA, Belém—Parà, pp 1–15
Rivano F (1992) La maladie sud-américaine des feuilles de l'hévéa. Etude, en conditions naturelles et contrôlées, des composants de la résistance partielle à Microcyclus ulei (P. Henn.) Arx. PhD thesis. Université de Paris-Sud, centre d'Orsay, France
Rivano F (1997a) La maladie sud-américaine des feuilles de l'hévéa. I. Variabilité du pouvoir pathogène de Microcyclus ulei. Plantations, Recherche, Développement, pp 104–110
Rivano F (1997b) La maladie sud-américaine des feuilles de l'hévéa. II. Evaluation précoce de la résistance des clones. Plantations, Recherche, Développement pp 187–193
Simmonds NW (1990) Breeding horizontal resistance to South American Leaf Blight of rubber. J Natural Rubber Res 5:102–113
Tabor GM, Kubisiak TL, Klopfenstein NB, Hall RB, Mac Nabb HSJ (2000) Bulked segregant analysis identifies molecular markers linked to Melampsora medusae resistance in Populus deltoides. Phytopathology 90:1039–1042
Van Ooijen JW (1999) LOD significance thresholds for QTL analysis in experimental populations of diploid species. Heredity 83:613–624
Van Ooijen JW, Maliepaard C (1996) MAPQTL version 3.0: software for the calculation of QTL positions on genetic maps. CPRO-DLO, Wageningen, The Netherlands
Villar M, Lefèvre F, Bradshaw HDJ, Teissier du Cros E (1996) Molecular genetics of rust resistance in poplars (Melampsora larici-populina Kleb/Populus sp.) by bulk segregant analysis in a 2×2 factorial mating design. Genetics 143:531–536
Wang GL, Mackill DJ, Bonman JM, Mc Couch SR, Champoux MC, Nelson RJ (1994) RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar. Genetics 136:1421–1434
Yang HY, Korban SS, Kruger J, Schmidt H (1997) The use of a modified bulk segregant analysis to identify a molecular marker linked to a scab resistance gene in apple. Euphytica 94:175–182
Zeigler RS, Tohme J, Nelson R (1994) Lineage exclusion: a proposal for linking blast population analysis to resistance breeding. In: Zeigler RS, Leong SA, Teng PS (eds) Rice blast disease. CAB International, pp 267–292
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We thank B. Courtois for critical reading of the manuscript, and C. Mattos for helpful comments. The experiments comply with the current laws of France where the experiments were performed.
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Communicated by C. Möllers
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Le Guen, V., Lespinasse, D., Oliver, G. et al. Molecular mapping of genes conferring field resistance to South American Leaf Blight (Microcyclus ulei) in rubber tree. Theor Appl Genet 108, 160–167 (2003). https://doi.org/10.1007/s00122-003-1407-9
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DOI: https://doi.org/10.1007/s00122-003-1407-9