Skip to main content
SpringerLink
Log in

Glycoalkaloids and acclimation capacity of hybrids between Solanum tuberosum and the incongruent hardy species Solanum commersonii

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

F1 and backcross hybrids between sexually incompatible species Solanum commersonii and Solanum tuberosum were characterized for glycoalkaloid content and capacity to cold acclimate. Glycoalkaloid (GA) analysis revealed that F1 triploids and BC1 pentaploids contained the glycoalkaloids of both parents. In BC2 (near) tetraploids the situation was different, in that some hybrids produced the GAs of both parents, whereas others contained only the GAs of S. tuberosum. This suggested that the GAs from S. commersonii may be lost rapidly, and that they may have a simple genetic control. The total tuber GA content of BC1 and BC2 groups averaged quite acceptable levels (165.9 mg/kg in BC1 and 192.8 mg/kg in BC2), with six genotypes having a GA content <200 mg/kg fresh weight. The F1 triploid hybrids expressed a capacity to cold acclimate similar to S. commersonii, whereas BC1 and BC2 genotypes generally displayed an acclimation capacity higher than the sensitive parent but lower than S. commersonii. However, one BC1 and two BC2 genotypes with an acclimation capacity as high as S. commersonii were identified. The polar lipid fatty acid composition in S. commersonii and its hybrid derivatives showed that, following acclimation, there was a significant increase in 18:3. Correlation analysis between the capacity to cold acclimate and the increase in 18:3 was significant, suggesting that the increase in 18:3 can be used as a biochemical marker for the assisted selection of cold-acclimating genotypes in segregating populations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.

Similar content being viewed by others

References

  • Carputo D, Barone A, Cardi T, Sebastiano A, Frusciante L, Peloquin SJ (1997) Endosperm Balance Number manipulation for direct in vivo germplasm introgression to potato from a sexually isolated relative (Solanum commersonii Dun.). Proc Natl Acad Sci USA 94:12,013–12,017

    Article  Google Scholar 

  • Carputo D, Cardi T, Palta JP, Sirianni P, Vega S, Frusciante L (2000) Tolerance to low temperatures and tuber soft rot in hybrids between Solanum commersonii and S. tuberosum obtained through manipulation of ploidy and endosperm balance number (EBN). Plant Breed 119:127–130

    Article  Google Scholar 

  • Chen YKH, Palta JP, Bamberg JB, Heiyoung K, Haberlach GT, Helgeson JP (1999a) Expression of non-acclimated freezing tolerance and cold acclimation capacity in somatic hybrids between hardy wild Solanum species and cultivated potatoes. Euphytica 107:1–8

    Article  Google Scholar 

  • Chen YKH, Bamberg JB, Palta JP (1999b) Expression of freezing tolerance in the interspecific F1 and somatic hybrids of potatoes. Theor Appl Genet 98:995–1004

    Article  Google Scholar 

  • Deahl KL, Sinden SL, Young RJ (1993) Evaluation of wild tuber-bearing Solanum accessions for foliar glycoalkaloid level and composition. Am Potato J 70:61–69

    CAS  Google Scholar 

  • Esposito F, Fogliano V, Cardi T, Carputo D, Filippone E (2001) Glycoalkaloid content and chemical composition of potatoes improved with non-conventional breeding approaches. J Agric Food Chem 50:1553–1561

    Article  Google Scholar 

  • Friedman M, McDonald GM (1997) Potato glycoalkaloids: chemistry, analyses, safety and plant physiology. Crit Rev Plant Sci 16:55–132

    Google Scholar 

  • Grassert V, Lellbach H (1987) Investigation of glycoalkaloid content of potato hybrids resistant to potato cyst eelworms Globodera rostochiensis and Globodera pallida. Biochem Biophys Pflanzen 182:473–479

    CAS  Google Scholar 

  • Hanneman RE Jr, Bamberg JB (1986) Inventory of tuber-bearing Solanum species. Univ Wisconsin Res Bull 533

  • Janácek J, Prásil I (1991) Quantification of plant frost injury by nonlinear fitting of an S-shaped function. Cryo-Lett 12:47–52

    Google Scholar 

  • Johnston SA, Hanneman RE (1982) Manipulations of Endosperm Balance Number overcome crossing barriers between diploid Solanum species. Science 217:446–448

    Google Scholar 

  • Kozukue N, Misoo S, Yamada T, Kamijima O, Friedman M (1999) Inheritance of morphological characters and glycoalkaloids in potatoes of somatic hybrids between dihaploid Solanum acule and tetraploid Solanum tuberosum. J Agric Food Chem 47:4478–4483

    Article  PubMed  Google Scholar 

  • Laurila J, Laakso I, Valkonen JPT, Hiltunen R, Pehu E (1996) Formation of parental-type and novel glycoalkaloids in somatic hybrids between Solanum brevidens and S. tuberosum. Plant Sci 118:145–155

    Article  Google Scholar 

  • Limin AE, Fowler DB (1989) The influence of cell size and chromosome dosage on cold-hardiness in the Triticeae. Genome 32:667–671

    Google Scholar 

  • Limin AE, Houde M, Chauvin LP, Fowler DB, Sarhan F (1995) Expression of the cold-induced wheat gene Wcs120 and its homologs in related species and interspecific combinations. Genome 38:1023–1031

    CAS  Google Scholar 

  • Maga JA (1994) Glycoalkaloids in the solanaceae. Food Rev Int 10:385–418

    CAS  Google Scholar 

  • McCollum GD, Sinden SL (1979) Inheritance study of tuber glycoalkaloids in a wild potato, Solanum chacoense Bitter. Am Potato J 95:95–113

    Google Scholar 

  • Ortiz R (1998) Potato breeding via ploidy manipulations. Plant Breed Rev 16:15–85

    Google Scholar 

  • Palta JP, Bruce D, Whitaker S, Weiss L (1993) Plasma membrane lipids associated with genetic variability in freezing tolerance and cold acclimation of Solanum species. Plant Physiol 103:793–803

    PubMed  Google Scholar 

  • Pavek JJ, Corsini DL (2001) Utilization of potato genetic resources in variety development. Am J Potato Res 78:433–441

    Google Scholar 

  • Ross H (1966) The use of wild Solanum species in German potato breeding of the past and today. Am Potato J 43:63–80

    Google Scholar 

  • Sanford LL, Sinden SL (1972) Inheritance of potato glycoalkaloids. Am Potato J 49:209–217

    CAS  Google Scholar 

  • Sanford LL, Deahl KL, Sinden SL, Kobayashi RS (1995) Glycoalkaloid content in tubers of hybrid and backcross populations from a Solanum tuberosum × S. chacoense cross. Am Potato J 72:261–270

    CAS  Google Scholar 

  • Sanford LL, Kowalski SP, Ronning CM, Deahl KL (1998) Leptines and other glycoalkaloids in tetraploid Solanum tuberosum × Solanum chacoense F2 hybrid and backcross families. Am J Potato Res 75:167–172

    CAS  Google Scholar 

  • Sarquis JI, Coria NA, Aguilar I, Rivera A (2000) Glycoalkaloid content in Solanum species and hybrids from a breeding program for resistance to late blight (Phytophthora infestans). Am J Potato Res 77:295–302

    CAS  Google Scholar 

  • Stone JM, Palta JP, Bamberg JB, Weiss LS, Harbage JF (1993) Inheritance of freezing resistance in tuber-bearing Solanum species: evidence for independent genetic control of non-acclimated freezing tolerance and cold acclimation capacity. Proc Natl Acad Sci USA 90:7869–7873

    PubMed  Google Scholar 

  • Uemura M, Yoshida S (1984) Involvement of plasma membrane alterations in cold acclimation of winter rye seedlings (Secale cereale L. cv Puma). Plant Physiol 75:818–826

    CAS  Google Scholar 

  • Valkonen JPT, Keskitalo M, Vasara T, Pietila L (1996) Potato glycoalkaloids: a burden or a blessing? Crit Rev Plant Sci 15:1–20

    Google Scholar 

  • Vàzquez A, Gonzàles G, Ferreira F, Moyana P, Kenne L (1997) Glycoalkaloids of Solanum commersonii Dun. ex Poir. Euphytica 95:195–201

    Article  Google Scholar 

  • Yencho GC, Kowalski SP, Kobayashi RS, Sinden SL, Bonierbale MW, Deahl KL (1998) QTL mapping of foliar glycoalkaloid aglycones in Solanum tuberosum × S. berthaultii potato progenies: quantitative variation and plant secondary metabolism. Theor Appl Genet 97:563–574

    CAS  Google Scholar 

Download references

Acknowledgements

Contribution no. 47 from DISSPA and no. 16 from CNR-IGV Portici. This study was partially supported by the Italian Ministry of Agriculture, in the context of the project "Miglioramento genetico della patata". The authors thank Dr. J. B. Bamberg for reviewing the manuscript, and R. Garramone for technical assistance.

Author information

Authors and Affiliations

  1. Department of Soil, Plant and Environmental Sciences (DISSPA), University of Naples "Federico II", Via Università 100, 80055 Portici, Italy

    D. Carputo, A. Barone & L. Frusciante

  2. Department of Food Science, University of Naples "Federico II", Parco Gussone, 80055, Portici, Italy

    A. Terra, F. Esposito & V. Fogliano

  3. Institute of Plant Genetics (IGV), Research Division of Portici, Via Università 133, 80055 Portici, Italy

    L. Monti

Authors
  1. D. Carputo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Terra
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Barone
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. F. Esposito
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. V. Fogliano
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. L. Monti
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. L. Frusciante
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to D. Carputo.

Additional information

Communicated by G. Wenzel

Rights and permissions

Reprints and permissions

About this article

Cite this article

Carputo, D., Terra, A., Barone, A. et al. Glycoalkaloids and acclimation capacity of hybrids between Solanum tuberosum and the incongruent hardy species Solanum commersonii . Theor Appl Genet 107, 1187–1194 (2003). https://doi.org/10.1007/s00122-003-1356-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-003-1356-3

Keywords

Search

Navigation