Identification of novel low M_r glutenin subunits in the high quality bread wheat cv Salmone and their effects on gluten quality

Abstract.

Southern-blot hybridization with a probe specific for genes encoding for low M_r glutenin subunits showed that the high quality bread wheat cv Salmone contains two DNA fragments designated as SF720 and SF750. These fragments were found to occur on the chromosome-1B satellite, and to be associated with the presence of two strongly staining low M_r glutenin subunits in the two-dimensional A-PAGE × SDS-PAGE pattern of cv Salmone. Comparison of 65 F₆ random lines derived from the cross between cv Salmone and the medium quality line FAP74809 revealed that the presence of fragments SF720 and SF750 had significant positive effects on several quality related parameters such as SDS sedimentation volume, Farinograph stability and Alveograph strength (W), tenacity (P) and elasticity (L). Additive effects of high M_r glutenin subunits 1 and 7+9 on gluten quality were found as well. Fragments SF720 and SF750 were suggested to occur at a locus other than Glu-B3, as indicated by their relatively high frequency of recombination with the Gli-B1 locus.