When flocking is costly: reduced cluster-flock density over long-duration flight in pigeons
Birds which fly in coordinated cluster-flocks can benefit through the formation of group-level structures and patterns which can deter predators by visual confusion. Though unlike V-formation flight, cluster-flocking increases the energetic cost of flight, particularly in denser flocks. Cluster formations therefore provide a unique opportunity to investigate trade-offs between increased work rate (e.g. higher flap frequency) and other benefits of flocking. As part of a routine 9-km training flight release, a flock of six homing pigeons (Columba livia) with 5 Hz GPS and 200 Hz accelerometer biologgers attached flew an alternative trajectory totalling 177 km and 256 min of flight. We provide the first evidence that during a long-duration flight, pigeons’ pairwise and group-level distances increased (i.e. group structure changed), while flap frequency decreased over time. This implies that as birds tire during long-duration flight, the ultimate functions of cluster-flocking—primarily anti-predator benefits—are overridden by the proximate costs of flying close to conspecifics.
KeywordsAerodynamics Biologging Collective behaviour Columba livia
The benefits for animals living in groups can include enhanced vigilance and predator detection (Elgar 1989; Parrish and Edelstein-Keshet 1999), increased foraging efficiency (Pitcher et al. 1982; Brown 1988) and the sharing of information about resources or efficient routes (Biro et al. 2006). In addition, for animals that travel in groups, potential energetic savings can be achieved through positive aero- or hydro-dynamic interactions that enhance the locomotor performance of individuals in the assemblage (Portugal et al. 2014; Voelkl et al. 2015). It has been demonstrated that for birds flying in a V-formation, for example, positive aerodynamic interactions between members of the flock allow individuals to maximise the capture of beneficial up-wash (Portugal et al. 2014). However, this aerodynamic power reduction due to group flight (for example, measured as a reduction in heart rate in flying white pelicans, Pelecanus onocrotalus; Weimerskirch et al. 2001) is not found in all bird groupings, with homing pigeons (Columba livia) flying in a cluster-flock having increased flap frequencies when flying near other birds (Usherwood et al. 2011). This increase in work rate is exacerbated for birds flying either at the back of the flock, or in denser formations, thus suggesting that the other aforementioned benefits of being in a group must outweigh the immediate energetic costs of cluster-flocking. Whether individuals in a flock will take measures to try and reduce the costs of flight in tight clusters—potentially by spreading out more—over long-duration flights is not currently known.
Flight is the most energetically costly form of aerobic locomotion in vertebrates, although it may be less costly in terms of energy expended per metre (i.e. the cost of transport) compared with walking or running due to the speed of travel (Alexander 2002). Nevertheless, long-duration flight is costly, and birds typically experience extensive changes in body composition before, during and after long flights (Wikelski et al. 2003). During long-duration flapping flight, it has been observed that heart rate can decrease as a function of flight time (Butler et al. 1998), assumed to be in response to the decrease in body mass that is observed in many species (Klaassen et al. 2000). For example, heart rate in migrating barnacle geese (Branta leucopsis) decreased by approximately 30% over the course of migration (Butler et al. 1998), while thrush nightingales (Luscinia luscinia) lost on average 3.82 g (13%) of their body mass during a 12-h flight in a wind tunnel (Klaassen et al. 2000). Thus, continuously measuring physiological and flight parameters in birds free-flying in a cluster has the potential to elucidate how individuals react to the changes in their physiology during long-duration flight, to establish if they attempt to reduce flight costs by spreading out from the cluster.
We analysed intra-group distances and flap frequency in a flock of six homing pigeons flying for 256 min over a 177-km journey that occurred for unknown reasons during a routine 9-km training flight. We aimed to investigate whether (a) individuals begin to spread out (increased distances to nearest neighbour and the group centroid) as flight duration increases, potentially to reduce the reported effects of flying in denser flocks, and (b) whether flap frequency decreases over time, to coincide with any increases in nearest neighbour distances.
Materials and methods
Each bird was tagged with both (1) a commercially available GPS logger (Qstarz BT-Q1300ST, Düsseldorf, Germany; logger mass 12 g) used to measure latitude, longitude and time (GMT), five times per second (5 Hz); and (2) Axivity AX3 accelerometer (Axivity Ltd., Newcastle upon Tyne, UK) loggers (mass 8 g) were used to measure three-dimensional acceleration at 200 Hz (Taylor et al. 2017; see Online Resource 1 for attachment protocols). The GPS units were programmed to “sports” mode, which is designed for use in fast activities involving high accelerations (up to 515 ms−1 speed, and up to 4푔 acceleration). For full calibration procedure and accuracy testing of the GPS loggers, see Brighton et al. (2017).
The birds had been released 12 times solo and three times as a group from the site, prior to the release in question. Each bird was equipped with GPS. The birds typically returned in 15.02 ± 41.93 min (median ± s.d.) for both solo and group flights combined, with an average group route efficiency of 0.43 ± 0.2 (median ± s.d.), where 1 is the beeline distance for the three group flights. The flight data for the extraordinary long flight presented took place on 18 July 2017, and the cause of the severe extended flight duration and deviation from a homeward heading is unknown. The birds were released at 13:30 BST. Upon evaluation of the GPS traces, additional weather parameters were gathered from three locations (see Online Resource 1 for full details) and assessed for repeatability to ensure dramatic changes in weather conditions were not responsible for the observed results. All parameters (wind direction, wind speed and gusts) were found to be consistent between locations (Online Resource 1).
Speed and neighbour-neighbour distance were both given by distance from one GPS position to another, using a spherical projection of the earth with radius = 63,710 km, speed, between an individual’s successive locations, and neighbour-neighbour distance between two individuals at the same timestamp. Flap frequency was identified from dorsal acceleration using the upper reversal point, dorsal body amplitude by integrating dorsal acceleration twice and applying a Butterworth filter. More metrics and more detailed descriptions of our calculations and equations are provided in the Online Resource 1.
We ran three models on each of our metrics over time: (1) linear models (LM); (2) linear mixed effects models, with individual as random intercept (LME-RI); and (3) linear mixed effects models with individual as random intercept and slope (LME-RIS) (Online Resource 1). Each model was run over 1000 bootstrap iterations to calculate average AIC values for model selection. Each iteration used AEPs (autocorrelation end points; Online Resource 1) as a sampling frequency to account for autocorrelation. The model structure with the lowest mean average AIC value was chosen as the best fit for the data. One thousand iterations of the chosen model were used to estimate R2, T value and lower and upper confidence intervals (CI; using the estimate for the fixed effect ± 1.96×Standard error). All statistics were calculated using R (R core team (2017))
Results and discussion
As the flock began to spread out further, flap frequency decreased by approximately 0.3 Hz. Usherwood et al. (2011) demonstrated that a tenfold increase in the spatial density of a pigeon flock was observed to be associated with a 0.1 Hz increase in wingbeat frequency. Similar effects have been observed more recently in flocking corvids, where flying in a group resulted in a 0.7 Hz increase in flap frequency versus flying solo (Ling et al. 2018). Recent work studying paired flight in homing pigeons found that individual spacing has a significant effect on wingbeat frequency over an extended range of distances, such that birds flying 50 m apart have an expected wingbeat frequency 0.54 Hz lower than those birds flying at 0 m spacing (Taylor et al. 2019), confirming firstly the importance of distances between individuals, but also confirming that the ~ 0.3 Hz flap frequency difference identified in the present study can be considered functionally significant.
The body mass loss after the long-duration flight was 22.23 ± 8.03 g (mean ± s.d.) for the six pigeons, with the respective measurements taken 7 days before and 2 days after the extended flight (Fig. 1b). We ran a linear mixed model with body mass as a dependent variable, and the date after the long flight as a different category than other data. Pigeon ID and Date were included as random intercepts. The long flight category significantly predicted a drop-in mass by an average of 32 g ± 7.5 g (s.e.m.) (LMM: DF = 53, t = − 4.30, p < 0.001). It is possible the reduction in flap frequency was a response to the expected loss in body mass. However, if body mass loss was the primary driver for the observed decline in flap frequency (Figs. 2b and d), we would expect flap frequency to continue to decrease over the solo flight period following the split, which was not the case. This strongly suggests that it is the absence of close neighbours that prompted the reduction in flap frequency, rather than the decrease in body mass. The two components, however, do not necessarily need to be mutually exclusive.
We thank the following people for useful discussions: Dora Biro, Emily Shepard, Liz Greenyer and Lucy Taylor.
Funding was provided by the Royal Society Research Grant (R10952) to S.J.P.
Compliance with ethical standards
Flight release protocol and husbandry conditions were approved by the Royal Holloway Ethical Review Board.
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