Abstract
The larvae of the sawfly Rhadinoceraea micans live and feed on a semi-aquatic plant, Iris pseudacorus, and their integument is strongly hydrophobic. The hydrophobicity is part of a chemical defence strategy, easy bleeding, also known from congeners. The prepupae burrow into the soil where they form a cocoon in which they pupate, thus implying different micro-environmental conditions. The cuticle structure and wetting defensive effectiveness of R. micans were compared between larvae and prepupae. The two stages were similarly well defended against attacking ants by the bleeding of a deterrent hemolymph, whereas they were dissimilar in the cuticle surface that presented sculptures and wax crystals at the larval stage only. The integument of prepupae was less structured, and hydrophilic. Larvae of R. micans exhibit, among sawflies, an exceptional cuticle structuring and we assume that they occupy this particular niche of a semi-aquatic environment to avoid encounters with ground-dwelling predators whereas prepupae may benefit from the chemical defence acquired at larval stage.
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References
Barker A (2006) Further descriptions of Dolerus larvae (Hymenoptera: Tenthredinidae), with notes on larval identification and feeding habits. In: Blank SM, Schmidt S, Taeger A (eds) Recent sawfly research: Synthesis and prospects. Goecke & Evers, Keltern, pp 83–96
Beneš K (1961) A contribution to the bionomics and morphology of Rhadinoceraea reitteri Knw. with a description of the larva (Hym., Tenthredinidae). Cas Cesk Spolecnosti Entomol 58:221–227
Beneš K (1973) The life history and juvenile stages of Rhadinoceraea bensoni Beneš (Hymenoptera, Tenthredinidae). Acta Entomol Bohemoslovaca 70:49–54
Boevé J-L (2009) Easily damaged integument of some sawflies (Hymenoptera) is part of a defence strategy against predators. In: Gorb SN (ed) Functional surfaces in biology: little structures with big effects, vol 1. Springer, New York, pp 31–43
Boevé J-L, Müller C (2005) Defence effectiveness of easy bleeding sawfly larvae towards invertebrate and avian predators. Chemoecology 15:51–58
Boevé J-L, Schaffner U (2003) Why does the larval integument of some sawfly species disrupt so easily? The harmful hemolymph hypothesis. Oecologia 134:104–111
Boevé J-L, Ducarme V, Mertens T, Bouillard P, Angeli S (2004) Surface structure, model and mechanism of an insect integument adapted to be damaged easily. J Nanobiotechnol 2(10):1–11
Boevé J-L, Voigt D, Gorb SN (2011) Crystalline wax coverage of the cuticle in easy bleeding sawfly larvae. Arthropod Struct Dev 40:185–189
Buckingham J (1994) Dictionary of natural products, vol. 7. Type of compound index, species index, 1st edn. Chapman & Hall, London
Burret M, Boevé J-L, Barker A, Spindler-Barth M (2005) Structure and mechanical strength of larval cuticle of sawflies capable of “easy bleeding”—a defence strategy against predators evolved in Tenthredinidae (Hymenoptera). Tissue Cell 37:67–74
Jia X (2006) Unsmooth cuticles of soil animals and theoretical analysis of their hydrophobicity and anti-soil-adhesion mechanism. J Colloid Interface Sci 295:490–494
Naumann ID, Balciunas JK (1997) A sawfly larva feeding on an aquatic fern (Hymenoptera: Symphyta: Pergidae). Aust Entomol 24:39–47
Ren L-Q, Tong J, Li J-Q, Chen B-C (2001) Soil adhesion and biomimetics of soil-engaging components: a review. J Agric Eng Res 79:239–263
Sägesser H, Lüscher M (1959) Über die Orientierung der Larve von Rhadinoceraea micans Kl. (Irisblattwespe). Rev Suisse Zool 66:343–346
Schaffner U, Boevé J-L, Gfeller H, Schlunegger UP (1994) Sequestration of Veratrum alkaloids by specialist Rhadinoceraea nodicornis Konow (Hymenoptera, Tenthredinidae) and its ecoethological implications. J Chem Ecol 20:3233–3250
Smith DR (1969) Nearctic sawflies. I. Blennocampinae: adults and larvae (Hymenoptera: Tenthredinidae). Tech Bull USDA 1397:1–176
Smith DR, Barrows EM (1995) Rhadinoceraea n. sp. (Hymenoptera: Tenthredinidae) from West Virginia, a second species on Zigadenus (Liliaceae). Entomol News 106:237–240
Smith DR, McDearman W (1990) A new Rhadinoceraea (Hymenoptera, Tenthredinidae) feeding on Zigadenus (Liliaceae) from Southeastern United States. Entomol News 101:13–19
Spindler-Barth M, Burret M, Spindler K-D (2005) “Easy bleeding” bearing relations to special cuticle structures in sawfly larvae (Hymenoptera: Tenthredinidae). Entomol Gener 27:257–268
Voigt D, Gorb SN, Boevé J-L (2011) Superhydrophobic cuticle with the pinning effect in the easy bleeder sawfly larva Rhadinoceraea micans (Hymenoptera, Tenthredinidae). Zoology 114:265–271
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We thank three reviewers for constructive comments on the manuscript, Frederik Hendrickx for help with the statistics, and Victoria Kastner for linguistic corrections.
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Communicated by: Sven Thatje
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Boevé, JL., Voigt, D. & Gorb, S.N. Integument and defence in larva and prepupa of a sawfly living on a semi-aquatic plant. Naturwissenschaften 100, 107–110 (2013). https://doi.org/10.1007/s00114-012-0998-x
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DOI: https://doi.org/10.1007/s00114-012-0998-x