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Dichotomy between Lactobacillus rhamnosus and Klebsiella pneumoniae on dendritic cell phenotype and function

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Abstract

The reaction of the intestinal immune system to intestinal bacteria shows striking differences between various bacterial strains. Whereas Klebsiella pneumoniae induces a fierce proinflammatory reaction, the probiotic strain Lactobacillus rhamnosus has clear anti-inflammatory effect in gastrointestinal disease and allergy. The molecular basis for this dichotomy is poorly understood but is likely to involve different modulation of antigen-presenting dendritic cells (DC) by L. rhamnosus and K. pneumoniae. Hence we evaluated phenotypic and functional characteristics of DC matured in the presence of L. rhamnosus and K. pneumoniae. Monocyte-derived immature DC were cultured in the presence of live bacteria to obtain mature DC. Both micro-organisms induced maturation of immature DC as shown by CD83 and CD86 expression, but receptors involved in activation of Th1 cells were expressed predominantly on DC exposed to K. pneumoniae. In contrast to K. pneumoniae, maturation with L. rhamnosus resulted in lower TNF-α, IL-6, and IL-8 production by immature DC and lower IL-12 and IL-18 production by mature DC. Moreover, L. rhamnosus led to the development of T cells without a typical Th phenotype whereas K. pneumoniae induced a Th1 immune response, dependent mainly on IL-12 production. Thus our results strongly support the concept that differential modulation of DC explains the differences in the immune response to various bacterial strains and indicates that K. pneumoniae induces Th1 immune responses via DC.

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Abbreviations

DC :

Dendritic cell

FACS :

Fluorescence-activated cell sorter

ICAM :

Intercellular adhesion molecule

IFN :

Interferon

IL :

Interleukin

LPS :

Lipopolysaccharide

MFI :

Mean fluorescence intensity

PAMP :

Pathogen associated molecular pattern

Th :

T helper cell

TNF :

Tumor necrosis factor

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Acknowledgements

The study is supported by an educational grant from Numico Research B.V. There is no conflict of interest on the part of any of the authors regarding this research presented here.

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Authors and Affiliations

  1. Academic Medical Center, Department of Experimental Internal Medicine, P.O. Box 22700, 1100 DE, Amsterdam, The Netherlands

    Henri Braat & Maikel P. Peppelenbosch

  2. Academic Medical Center, Department of Cell Biology and Histology and Dermatology, P.O. Box 22700, 1100 DE , Amsterdam, The Netherlands

    Esther C. de Jong & Martien L. Kapsenberg

  3. Academic Medical Center, Department of Gastroenterology and Hepatology, P.O. Box 22700, 1100 DE, Amsterdam, The Netherlands

    Jan M. H. van den Brande & Sander J. H. van Deventer

  4. Numico Research B.V., Department of Gastrointestinal Biology, P.O. Box 7005, 6700 CA, Wageningen, The Netherlands

    Eric A. F. van Tol

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  1. Henri Braat
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Correspondence to Henri Braat.

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Braat, H., de Jong, E.C., van den Brande, J.M.H. et al. Dichotomy between Lactobacillus rhamnosus and Klebsiella pneumoniae on dendritic cell phenotype and function. J Mol Med 82, 197–205 (2004). https://doi.org/10.1007/s00109-003-0509-9

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