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Primäre nasale Epithelzellen und Fibroblasten haben entzündungsfördernde Eigenschaften

Primary nasal epithelial cells and fibroblasts have inflammation-inducing functions

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Zusammenfassung

Hintergrund.

Akute Entzündungen der Nasenschleimhäute führen zu einer lokalen Immunantwort mit Einstrom von neutrophilen Granulozyten in das Gewebe. Der Mechanismus bakterieller Entzündungen in der Nasenschleimhaut ist bisher unbekannt.

Patienten und Methode.

Um zu untersuchen, ob Bakterien Neutrophile anlockende Proteine, sog. Chemotaxine, induzieren, wurden primäre Epithelzell- und Fibroblastenkulturen (n=4) von Nasenschleimhäuten angezüchtet. Nach 24 und 72 h Inkubationszeit mit einem proinflammatorischen Zytokin TNF-α (Tumornekrosefaktor α) und Überständen von Pseudomonas-aeruginosa-Bakterienstämmen (PA01) wurde im ELISA-Verfahren die Proteinkonzentration der C-X-C-Chemokine Interleukin- (IL-) 8, GRO-α ("growth-related oncogene α") und ENA-78 ("epithelial neutrophil-activating peptide") im Überstand gemessen.

Ergebnisse.

In vitro mit bakteriellen Überständen stimulierte primäre nasale Fibroblasten und Epithelzellen synthetisierten IL-8 und GRO im Nanogrammbereich.

Schlussfolgerung.

Erstmals konnte gezeigt werden, dass Überstände von Pseudomonas aeruginosa in nasalen Epithelzellen und Fibroblasten das Entzündungsgeschehen durch die Synthese von Chemokinen, IL-8 und GRO-α, unterstützen.

Abstract

Background.

The appearance of neutrophils in rhinitis and sinusitis led to the working hypothesis that neutrophil-specific attractants commonly called chemokines are generated by stimulation with proinflammatory cytokines and bacteria. The receptor mechanism of chemokine synthesis by bacterial products is under discussion and still has to be elucidated.

Patients and Methods.

The primary nasal cultures of epithelial cells and fibroblasts (n=4) were incubated with TNF-α (tumor necrosis factor) for 24 and 72 h. Bacterial stimulation of the cell cultures was performed by adding supernatants from the mucoid phenotype of Pseudomonas aeruginosa (PA01) in dilution 1:5 for 24 and 72 h. Supernatants were collected and the concentration of the chemokines interleukin-8 (IL-8), GRO-α (growth-related oncogene-α), and ENA-78 (epithelial neutrophil-activating peptide) were determined by ELISA technique. Our results revealed that the protein concentration of the chemokines GRO-α and IL-8 was upregulated by TNF-α as well as by bacterial supernatants in epithelial cells.

Conclusion.

We conclude that GRO-α and IL-8 were inducible by bacterial supernatants in nasal epithelial cells and fibroblasts.

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Danksagung.

Für die Anzüchtung und Bebrütung der Pseudomonaskulturen möchten wir Herrn Univ. Prof. Dr. G. Doering und Herrn Dr. D. Worlitzsch vom Institut für Hygiene der Medizinischen Fakultät der Universität Tübingen danken.

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Author notes

  1. C. Rudack

    Present address: HNO-Klinik, Universitätsklinikum, Münster, Kardinal-von-Galen-Ring 10, 48149, Deutschland

Authors and Affiliations

  1. HNO-Klinik, Universitätsklinikum, Münster, Deutschland

    C. Rudack, W. Stoll & W. Hermann

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  1. C. Rudack
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Correspondence to C. Rudack.

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Rudack, C., Stoll, W. & Hermann, W. Primäre nasale Epithelzellen und Fibroblasten haben entzündungsfördernde Eigenschaften. HNO 51, 480–485 (2003). https://doi.org/10.1007/s00106-002-0754-1

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