Skip to main content

Advertisement

SpringerLink
Log in

Characterization of phytochemical composition and bioactivity assessment of Pseudotrachydium kotschyi essential oils

  • Original Research
  • Published:
Medicinal Chemistry Research Aims and scope Submit manuscript

Abstract

Pseudotrachydium kotschyi is a native medicinal plant used as a local medicine from Lorestan province of western Iran having numerous biological properties. After GC−MS analysis of Pseudotrachydium kotschyi essential oils (PKEOs), some biological activities were investigated. Additionally, TNF-α and TGF-β1 were studied in Leishmania-infected macrophages (in vitro) and Carrageenan-induced paw edema in rats. Finally, anti-inflammatory and wound-healing potential of PKEOs were investigated in vivo in mice. Total components identified by GC−MS contained 53 molecules, among which Z-α-trans-Bergamotol (23.25%), Durylaldehyde (16.07%), and α-Bergamotene (10.48%) existed in the highest amount in PKEOs. Antimicrobial assay of PKEOs showed maximum growth inhibition against Listeria monocytogenes and Kluyveromyces marxianus at the dose of 625 μg/ml. PKEOs exhibited a high cytotoxicity on lung cancer cell line A549 at a dose of 4 mg/ml. Anti-Leishmania activity of PKEOs showed concentration of 5000 µg/ml that had similar potency of glucantime at 125 µg/ml. The highest anti-inflammatory effect of PKEOs for Carrageenan-induced paw edema was observed at 100 concentrations, with paw inflammation reduced by 83.70% after using PKEO ointment. The present study reports broad-spectrum biological functions for essential oil from P. kotschyi. However, these biological activities, including anti-proliferative, anti-Leishmania, anti-inflammatory, antimicrobial and wound-healing potential, were not studied in detail. Therefore, it may be attractive for investigating the detailed mechanistic and molecular aspects of biological activities of PKEOs. Considering that the present study is the first to report the biological activity of P. kotschyi, further studies on their metabolites are needed to replicate the present findings.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10

Similar content being viewed by others

References

  • Amiri MS, Joharchi MR (2016) Ethnobotanical knowledge of Apiaceae family in Iran: a review. Avicenna J Phytomed 6:621

    PubMed  PubMed Central  Google Scholar 

  • Ashour M, Wink M, Gershenzon J (2018) Biochemistry of terpenoids: monoterpenes, sesquiterpenes and diterpenes. Ann Plant Rev online 40:258–303

    Article  Google Scholar 

  • Ashrafi B, Rashidipour M, Marzban A, Soroush S, Azadpour M, Delfani S, Ramak P (2019) Mentha piperita essential oils loaded in a chitosan nanogel with inhibitory effect on biofilm formation against S. mutans on the dental surface. Carbohydr Polym 212:142–149

    Article  CAS  Google Scholar 

  • Aziz-UL-Ikram NBZ, Shinwari ZK, Qaiser M (2015) Ethnomedicinal review of folklore medicinal plants belonging to family Apiaceae of Pakistan. Pak J Bot 47:1007–1014

    CAS  Google Scholar 

  • Barati M, Sharifi I, Sharififar F, Hakimi Parizi M, Shokri A (2014) Anti-leishmanial activity of gossypium hirsutum L., Ferula assa-foetida L. and Artemisia aucheri Boiss. Extracts by colorimetric assay. Anti-Infect Agents 12:159–164

    Article  CAS  Google Scholar 

  • Bartikova H, Hanusova V, Skalova L, Ambroz M, Bousova I (2014) Antioxidant, pro-oxidant and other biological activities of sesquiterpenes. Curr Top Med Chem 14:2478–2494

    Article  CAS  Google Scholar 

  • Borg-Karlson A-K, Valterová I, Nilsson LA (1993) Volatile compounds from flowers of six species in the family Apiaceae: bouquets for different pollinators? Phytochemistry 35:111–119

    Article  Google Scholar 

  • Caputi L, Aprea E (2011) Use of terpenoids as natural flavouring compounds in food industry. Recent Pat Food Nutr Agric 3:9–16

    Article  CAS  Google Scholar 

  • Dahham S, Tabana Y, Iqbal M, Ahamed M, Ezzat M, Majid A, Majid A (2015) The anticancer, antioxidant and antimicrobial properties of the sesquiterpene β-caryophyllene from the essential oil of Aquilaria crassna. Molecules 20:11808–11829

    Article  CAS  Google Scholar 

  • de Lima EM, Cazelli DSP, Pinto FE, Mazuco RA, Kalil IC, Lenz D, Scherer R, de Andrade TU, Endringer DC (2016) Essential oil from the resin of Protium heptaphyllum: chemical composition, cytotoxicity, antimicrobial activity, and antimutagenicity. Pharmacogn Mag 12:S42

    Article  Google Scholar 

  • Denizot F, Lang R (1986) Rapid colorimetric assay for cell growth and survival: modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods 89:271–277

    Article  CAS  Google Scholar 

  • Elghwaji W, El-Sayed AM, El-Deeb KS, ElSayed AM (2017) Chemical composition, antimicrobial and antitumor potentiality of essential oil of Ferula tingitana L. Apiaceae grow in Libya. Pharmacogn Mag 13:S446

    PubMed  PubMed Central  Google Scholar 

  • Fabricant DS, Farnsworth NR (2001) The value of plants used in traditional medicine for drug discovery. Environ Health Perspect 109:69–75

    CAS  PubMed  PubMed Central  Google Scholar 

  • Fang H, Shang T, Xiao P, Pu Q (1990) Studies on chemical constituents of the essential oil of Cyclorhiza waltonii. Yao xue xue bao= Acta Pharm Sin 25:534–537

    CAS  Google Scholar 

  • Gallily R, Yekhtin Z, Hanuš LO (2018) The anti-inflammatory properties of terpenoids from cannabis. Cannabis Cannabinoid Res 3:282–290

    Article  CAS  Google Scholar 

  • Gupta AK, Parasar D, Sagar A, Choudhary V, Chopra BS, Garg R, Khatri N (2015) Analgesic and anti-inflammatory properties of gelsolin in acetic acid induced writhing, tail immersion and carrageenan induced paw edema in mice. PLoS ONE 10:e0135558

    Article  Google Scholar 

  • Gyawali R, Ryu K-Y, Shim S-L, Kim J-H, Seo H-Y, Han K-J, Kim K-S (2006) Essential oil constituents of Swertia chirata Buch.-Ham. Prev Nutr Food Sci 11:232–236

    Article  CAS  Google Scholar 

  • Hua K-F, Yang T-J, Chiu H-W, Ho C-L (2014) Essential oil from leaves of Liquidambar formosana ameliorates inflammatory response in lipopolysaccharide-activated mouse macrophages. Nat Prod Commun 9:1934578X1400900638

    Google Scholar 

  • Iqbal J, Abbasi BA, Mahmood T, Kanwal S, Ali B, Shah SA, Khalil AT (2017) Plant-derived anticancer agents: a green anticancer approach. Asian Pac J Trop Biomed 7:1129–1150

    Article  Google Scholar 

  • Kheirandish F, Delfan B, Mahmoudvand H, Moradi N, Ezatpour B, Ebrahimzadeh F, Rashidipour M (2016) Antileishmanial, antioxidant, and cytotoxic activities of Quercus infectoria Olivier extract. Biomed Pharmacother 82:208–215

    Article  CAS  Google Scholar 

  • Lu X, Tang K, Li P (2016) Plant metabolic engineering strategies for the production of pharmaceutical terpenoids. Front Plant Sci 7:1647

    PubMed  PubMed Central  Google Scholar 

  • Mohammadzadeh A, Farnia P, Ghazvini K, Behdani M, Rashed T, Ghanaat J (2006) Rapid and low-cost colorimetric method using 2, 3, 5-triphenyltetrazolium chloride for detection of multidrug-resistant Mycobacterium tuberculosis. J Med Microbiol 55:1657–1659

    Article  CAS  Google Scholar 

  • Mozaffarian V (2007) Flora of Iran, No. 54: Umbelliferae. Research Institute of Forests and Rangelands, Tehran. (in Persian)

  • Nascimento JC, Barbosa LC, Paula VF, David JM, Fontana R, Silva LA, França RS (2011) Chemical composition and antimicrobial activity of essential oils of Ocimum canum Sims. and Ocimum selloi Benth. Acad Bras Ciênc 83:787–800

    Article  CAS  Google Scholar 

  • Pimenov M, Kljuykov E (2000) Taxonomic revision of Pleurospermum Hoffm. and related genera of Umbelliferae II. The genera Pleurospermum, Pterocyclus, Trachydium, Keraymonia, Pseudotrachydium, Aulacospermum, and Hymenolaena. Feddes Repert 111:517–534

    Article  Google Scholar 

  • Pino JA, Rosado A, Fuentes V (1997) Composition of the leaf oil of Eryngium foetidum L. from Cuba. J Ess Oil Res 9:467–468

    Article  CAS  Google Scholar 

  • Pradhan D, Panda P, Tripathy G (2009) Wound healing activity of aqueous and methanolic bark extracts of vernonia arborea Buch.-Ham. in wistar rats. Nat Prod Radiance 8:6–11

    Google Scholar 

  • Prakash V (2018) Terpenoids as cytotoxic compounds: a perspective. Pharmacogn Rev 12:24

    Article  Google Scholar 

  • Sardrood SG, Saadatmand S, Assareh MH, Satan TN (2019) Chemical Composition and biological activity of essential oils of Centella asiatica (L.). Toxicol Environ Health Sci 11:125–131

    Article  Google Scholar 

  • Sayyedrostami T, Pournaghi P, Vosta-Kalaee SE, Zangeneh MM (2018) Evaluation of the wound healing activity of Chenopodium botrys leaves essential oil in rats (a short-term study). J Ess Oil Bear Plants 21:164–174

    Article  CAS  Google Scholar 

  • Sharma A, Bajpai VK, Shukla S (2013) Sesquiterpenes and cytotoxicity. In: Ramawat KG, Mérillon J-M (eds), Natural products: phytochemistry, botany and metabolism of alkaloids, phenolics and terpenes. Springer, Berlin, pp 3515−3550

  • Silva J, Abebe W, Sousa S, Duarte V, Machado M, Matos F (2003) Analgesic and anti-inflammatory effects of essential oils of Eucalyptus. J Ethnopharmacol 89:277–283

    Article  CAS  Google Scholar 

  • Sobral MV, Xavier AL, Lima TC, de Sousa DP (2014) Antitumor activity of monoterpenes found in essential oils. Sci World J 2014:953451

    Article  Google Scholar 

  • Sonboli A, Kanani MR, Yousefzadi M, Mojarrad M (2007) Biological activity and composition of the essential oil of Tetrataenium nephrophyllum (Apiaceae) from Iran. Nat Prod Commun 2:1934578X0700201212

    Google Scholar 

  • Swamy MK, Akhtar MS, Sinniah UR (2016) Antimicrobial properties of plant essential oils against human pathogens and their mode of action: an updated review. Evid Based Complement Alternat Med 2016:3012462

    Article  Google Scholar 

  • Wagner VP, Webber LP, Ortiz L, Rados PV, Meurer L, Lameira OA, Lima RR, Martins MD (2017) Effects of Copaiba oil topical administration on oral wound healing. Phytother Res 31:1283–1288

    Article  CAS  Google Scholar 

  • Wang P, Su Z, Yuan W, Deng G, Li S (2012) Phytochemical constituents and pharmacological activities of Eryngium L. (Apiaceae). Pharm Crop 3:99–120

    Article  CAS  Google Scholar 

  • Wang Y-T, Zhu L, Zeng D, Long W, Zhu S-M (2016) Chemical composition and anti-inflammatory activities of essential oil from Trachydium roylei. J Food Drug Anal 24:602–609

    Article  CAS  Google Scholar 

  • Wayne P (2011) Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility testing

Download references

Acknowledgements

We thank Dr. Nasim Beigi Boroujeni for valuable advice in the interpretation of histological study. We would also like to appreciate Dr. Mohammad Mehrnai for the plant characterization. The research project; A-10-1471-2 [ID:261] has been financially supported by Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Iran.

Author information

Authors and Affiliations

  1. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

    Behnam Ashrafi, Fatemeh Beyranvand, Fatemeh Ashouri, Abdolrazagh Marzban, Farnaz Kheirandish & Somayeh Shahrokhi

  2. Nutritional Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

    Marzieh Rashidipour

  3. Department of Medical Parasitology and Mycology, Lorestan University of Medical Sciences, Khorramabad, Iran

    Farnaz Kheirandish

  4. Lorestan Agricultural and Natural Resources Research and Education Center Department of Animal Science, Khorramabad, Iran

    Saeed Veiskarami

  5. Research Division of Natural Resources, Lorestan Agricultural and Natural Resources Research and Education Center, AREEO, Khorramabad, Iran

    Parvin Ramak

  6. Department of Immunology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

    Somayeh Shahrokhi

Authors
  1. Behnam Ashrafi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fatemeh Beyranvand
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fatemeh Ashouri
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marzieh Rashidipour
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Abdolrazagh Marzban
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Farnaz Kheirandish
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Saeed Veiskarami
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Parvin Ramak
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Somayeh Shahrokhi
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

BA designed the study as a major researcher. FB and FA contributed to animal studies, especially histology studies. MR performed essential oil extraction by Clevenger apparatus. AM was responsible for data arrangements, scientific writing, and graphical designing. FK performed anti-leishmania studies and cytokine assay. SV analyzed all the data statistically. PR was responsible for the plant and SS was responsible for study design, project management, and reviewing the manuscript.

Corresponding author

Correspondence to Somayeh Shahrokhi.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ashrafi, B., Beyranvand, F., Ashouri, F. et al. Characterization of phytochemical composition and bioactivity assessment of Pseudotrachydium kotschyi essential oils. Med Chem Res 29, 1676–1688 (2020). https://doi.org/10.1007/s00044-020-02594-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00044-020-02594-5

Keywords

Search

Navigation