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Labdane diterpene lactones of Vitex pubescens and their antileukemic properties

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Abstract

Vitex pubescens belonging to the Verbenaceae family, has been historically used as a folk medicine in Indonesia. The dichloromethane extract of V. pubescens stembarks interfered cell proliferation and induced apoptosis in human leukemia HL-60 cells. Our phytochemical investigation resulted in the isolation of several constituents and their chemical structures were determined by spectroscopic analyses. Upon treatment with labdane diterpene lactones, relatively high inhibition of cell proliferation and apoptotic response of the cells were observed. Furthermore, the structure-activity relationship analysis with clerodane diterpenes revealed that a γ-butyrolactone ring contributes their antileukemic potency. Overall, these findings enhanced the ethnopharmacological relevance of V. pubescens for cancer chemotherapy.

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Acknowledgements

We would like to thank to Directorate General of Higher Education for the grant through BPPDN scholarship.

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Authors and Affiliations

  1. Department of Mathematics and Natural Science Education, Riau University, Pekanbaru,, Riau, Padang, Indonesia

    Lenny Anwar

  2. Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Andalas, 25163, Limau Manis, Padang, Indonesia

    Lenny Anwar, Mai Efdi & Sanusi Ibrahim

  3. Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Masayuki Ninomiya & Mamoru Koketsu

  4. Faculty of Pharmacy, Universitas Andalas, 25163, Limau Manis, Padang, Indonesia

    Deddi Prima Putra

  5. Division of Anaerobe Research, Life Science Research Center, Gifu University, 1-1 Yanagido, Gifu, 501-1194, Japan

    Kaori Tanaka

  6. United Graduate School of Drug Discovery and Medicinal Information Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1194, Japan

    Kaori Tanaka

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  1. Lenny Anwar
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  2. Mai Efdi
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  3. Masayuki Ninomiya
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  7. Mamoru Koketsu
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Correspondence to Mamoru Koketsu.

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Anwar, L., Efdi, M., Ninomiya, M. et al. Labdane diterpene lactones of Vitex pubescens and their antileukemic properties. Med Chem Res 26, 2357–2362 (2017). https://doi.org/10.1007/s00044-017-1937-3

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  • DOI: https://doi.org/10.1007/s00044-017-1937-3

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