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Evaluation of silver-doped indium oxide nanoparticles as in vitro α-amylase and α-glucosidase inhibitors

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Abstract

Pristine and 2 % silver-doped indium oxide (In2O3) nanoparticles, synthesized by solution combustion method, yielded spherical nanoparticles in the range of 20–30 nm. The nanoparticles were stabilized in cubic bixbyite structure as revealed from X-ray diffraction study. In order to evaluate the potential of these nanoparticles to modulate enzyme activity, α-amylase and α-glucosidase were used as model enzymes. Pristine and 2 % silver-doped In2O3 nanoparticles demonstrated dose-dependent inhibition of α-amylase and α-glucosidase activities. Pristine In2O3 nanoparticles demonstrated 26.4 % (300 µg/mL) and 65.3 % (300 µg/mL) inhibition against α-amylase and α-glucosidase, respectively. In contrast, silver-doped In2O3 nanoparticles depicted 94.1 % (300 µg/mL) and 99.6 % (0.18 µg/mL) inhibition against α-amylase and α-glucosidase, respectively. In comparison with acarbose, a standard anti-diabetic drug that depicted absolute inhibition of α-glucosidase activity at 300 µg/mL, 2 % silver-doped In2O3 nanoparticles completely inhibited α-glucosidase at a very low concentration (0.18 µg/mL). In view of our results, the activity of α-amylase and α-glucosidase, which are targets for treatment of type 2 diabetes, can be modulated using silver-doped In2O3 nanoparticles in the concentration-dependent manner. Therefore, silver-doped In2O3 has a potential to be used as a prospective starch blocker.

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Acknowledgments

The authors are thankful to University Grants Commission—Basic Scientific Research (UGC-BSR) program for providing financial support to Ms. M. Z. Naik and Department of Biotechnology (DBT), Government of India, for providing Senior Research Fellowship (SRF) to Mr. S. N. Meena.

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Authors and Affiliations

  1. Department of Chemistry, Goa University, Goa, 403206, India

    M. Z. Naik & A. V. Salker

  2. Department of Biotechnology, Goa University, Goa, 403206, India

    S. N. Meena & S. C. Ghadi

  3. Department of Microbiology, Goa University, Goa, 403206, India

    M. M. Naik

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  1. M. Z. Naik
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  2. S. N. Meena
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Correspondence to A. V. Salker.

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Naik, M.Z., Meena, S.N., Ghadi, S.C. et al. Evaluation of silver-doped indium oxide nanoparticles as in vitro α-amylase and α-glucosidase inhibitors. Med Chem Res 25, 381–389 (2016). https://doi.org/10.1007/s00044-015-1494-6

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  • DOI: https://doi.org/10.1007/s00044-015-1494-6

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