Skip to main content

Advertisement

SpringerLink
Log in

Biosynthesis of Silver Nanoparticles: Preparation, Optimization and In Vitro Anti-diabetic Effect

  • Published:
BioNanoScience Aims and scope Submit manuscript

Abstract

Biosynthesized silver nanoparticles (Ag-NPs) displayed altogether particular physical, concoction, and natural properties, drawing high consideration for a variety of new applications in different fields due to their cost-effective and eco-friendly procedure. The current work delineates a methodology for the green synthesis of Ag-NPs from the roots of Pueraria Lobata. The Ag-NPs were synthesized at room temperature through a reduction method from the methanolic crude extract of the roots of Pueraria Lobata. UV–vis absorption spectra of Ag-NPs demonstrated the synthesis by giving an intense peak at 248 nm. Transmission electron microscopy (TEM) revealed the synthesis of Ag-NPs was spherical with a size range of 30–60 nm. Fourier transformation infrared spectroscopy (FTIR) results showed the binding properties of bio-constituents responsible for stabilizing the nanoparticles. The synthesis method of Ag-NPs was optimized and the most stable Ag-NPs were achieved after 13 h at pH 9. The in vitro anti-diabetic ability of Ag-NPs was tested against carbohydrate digestion enzyme α-amylase. The result suggests that the Ag-NPs demonstrated remarkable potential of anti-diabetic activity against the key enzyme of diabetes and were found to be appropriate for nano bio-medical application.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Zhang, X. F., Liu, Z. G., Shen, W., & Gurunathan, S. (2016). Silver nanoparticles: Synthesis, characterization, properties, applications, and therapeutic approaches. International Journal of Molecular Sciences, 17(9), 1534.

    Article  Google Scholar 

  2. Li, W. R., Xie, X. B., Shi, Q. S., Zeng, H. Y., You-Sheng, O. Y., & Chen, Y. B. (2010). Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli. Applied Microbiology and Biotechnology, 85(4), 1115–1122.

    Article  Google Scholar 

  3. Gurunathan, S., Park, J. H., Han, J. W., & Kim, J. H. (2015). Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: Targeting p53 for anticancer therapy. International Journal of Nanomedicine, 10, 4203.

    Article  Google Scholar 

  4. Li, C., Zhang, Y., Wang, M., Zhang, Y., Chen, G., Li, L., Wu, D., & Wang, Q. (2014). In vivo real-time visualization of tissue blood flow and angiogenesis using Ag2S quantum dots in the NIR-II window. Biomaterials, 35(1), 393–400.

    Article  Google Scholar 

  5. Sondi, I., & Salopek-Sondi, B. (2004). Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science, 275(1), 177–182.

    Article  Google Scholar 

  6. Sharma, V. K., Yngard, R. A., & Lin, Y. (2009). Silver nanoparticles: Green synthesis and their antimicrobial activities. Advances in Colloid and Interface Science, 145(1–2), 83–96.

    Article  Google Scholar 

  7. Janakiraman, V., Govindarajan, K., & Magesh, C. R. (2019). Biosynthesis of silver nanoparticles from endophytic fungi, and its cytotoxic activity. BioNanoScience, 9(3), 573–579.

    Article  Google Scholar 

  8. Singh, R., Hano, C., Nath, G., & Sharma, B. (2021). Green biosynthesis of silver nanoparticles using leaf extract of Carissa carandas L. and their antioxidant and antimicrobial activity against human pathogenic bacteria. Biomolecules, 11(2), 299.

    Article  Google Scholar 

  9. Saqib, N. U., & Rahim, M. (2016). Toxicity of silver nanoparticles. Madridge Journal of Nano Technology, 1(1), 1–2.

    Article  Google Scholar 

  10. Kaur, G., Kalia, A., & Sodhi, H. S. (2020). Size controlled, time-efficient biosynthesis of silver nanoparticles from Pleurotus florida using ultra-violet, visible range, and microwave radiations. Inorganic and Nano-Metal Chemistry, 50(1), 35–41.

    Article  Google Scholar 

  11. Azizi, M., Sedaghat, S., Tahvildari, K., Derakhshi, P., & Ghaemi, A. (2020). Green biosynthesis of silver nanoparticles with Eryngium caucasicum Trautv aqueous extract. Inorganic and Nano-Metal Chemistry, 50(6), 429–436.

    Article  Google Scholar 

  12. Otari, S. V., Patil, R. M., Nadaf, N. H., Ghosh, S. J., & Pawar, S. H. (2012). Green biosynthesis of silver nanoparticles from an actinobacteria Rhodococcus sp. Materials Letters, 72, 92–94.

    Article  Google Scholar 

  13. Mittal, A. K., Chisti, Y., & Banerjee, U. C. (2013). Synthesis of metallic nanoparticles using plant extracts. Biotechnology Advances, 31(2), 346–356.

    Article  Google Scholar 

  14. Shrivastava, S., & Leelavathi, S. (2010). Preliminary phytochemical evaluation of leaf extracts of Catunaregum spinosa Thumb. International Journal of Pharmaceutical Sciences Review and research, 3(2), 114–118.

    Google Scholar 

  15. Carter, G. A., & Teramura, A. H. (1988). Vine photosynthesis and relationships to climbing mechanics in a forest understory. American Journal of Botany, 75(7), 1011–1018.

    Article  Google Scholar 

  16. Hu, H. T., Fen, F., & Ding, M. P. (2008). Effects of puerarin with aspirin on the markers of damaged vascular endothelial cells in patients with acute cerebral infarction. Zhongguo Zhong Yao Za Zhi= Zhongguo Zhongyao Zazhi= China Journal of Chinese Materia Medica, 33(23), 2827–2829.

    Google Scholar 

  17. Ren, P., Hu, H., & Zhang, R. (2000). Observation on efficacy of puerarin in treating diabetic retinopathy. Zhongguo Zhong Xi Yi Jie He Za Zhi Zhongguo Zhongxiyi Jiehe Zazhi= Chinese Journal of Integrated Traditional and Western Medicine, 20(8), 574–576.

    Google Scholar 

  18. Wu, K., Liang, T., Duan, X., Xu, L., Zhang, K., & Li, R. (2013). Anti-diabetic effects of puerarin, isolated from Pueraria lobata (Willd.), on streptozotocin-diabetogenic mice through promoting insulin expression and ameliorating metabolic function. Food and chemical toxicology, 60, 341–347.

    Article  Google Scholar 

  19. Long, A. N., & Dagogo-Jack, S. (2011). Comorbidities of diabetes and hypertension: Mechanisms and approach to target organ protection. The Journal of Clinical Hypertension, 13(4), 244–251.

    Article  Google Scholar 

  20. Newton, C. A., & Raskin, P. (2004). Diabetic ketoacidosis in type 1 and type 2 diabetes mellitus: Clinical and biochemical differences. Archives of Internal Medicine, 164(17), 1925–1931.

    Article  Google Scholar 

  21. Talchai, C., Xuan, S., Lin, H. V., Sussel, L., & Accili, D. (2012). Pancreatic β cell dedifferentiation as a mechanism of diabetic β cell failure. Cell, 150(6), 1223–1234.

    Article  Google Scholar 

  22. McCue, P. P., & Shetty, K. (2004). Inhibitory effects of rosmarinic acid extracts on porcine pancreatic amylase in vitro. Asia Pacific Journal of Clinical Nutrition, 13(1), 101–106.

    Google Scholar 

  23. Ihsan, M., Niaz, A., Rahim, A., Zaman, M. I., Arain, M. B., Sharif, T., & Najeeb, M. (2015). Biologically synthesized silver nanoparticle-based colorimetric sensor for the selective detection of Zn 2+. RSC advances, 5(111), 91158–91165.

    Article  Google Scholar 

  24. Eichler, H. G., Korn, A., Gasic, S., Pirson, W., & Businger, J. (1984). The effect of a new specific α-amylase inhibitor on post-prandial glucose and insulin excursions in normal subjects and type 2 (non-insulin-dependent) diabetic patients. Diabetologia, 26(4), 278–281.

    Article  Google Scholar 

  25. Justino, A. B., Miranda, N. C., Franco, R. R., Martins, M. M., da Silva, N. M., & Espindola, F. S. (2018). Annona muricata Linn. leaf as a source of antioxidant compounds with in vitro antidiabetic and inhibitory potential against α-amylase, α-glucosidase, lipase, non-enzymatic glycation and lipid peroxidation. Biomedicine & Pharmacotherapy, 100, 83–92.

    Article  Google Scholar 

Download references

Funding

This study received financial support from the Department of Chemistry, Zhejiang University, Hangzhou, and the Department of Chemistry, University of Science and Technology Bannu.

Author information

Authors and Affiliations

  1. Department of Chemistry, Zhejiang University, Hangzhou, 310028, People’s Republic of China

    Muhammad Inam

  2. Department of Chemistry, University of Science and Technology Bannu, Banu, 28100, Pakistan

    Afzal Shah & Wahib Noor Khan

  3. Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan

    Sumaira Sharif

  4. Department of Chemistry, University of Buner, Buner, 17290, KP, Pakistan

    Najm Us Saqib

Authors
  1. Muhammad Inam
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Afzal Shah
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wahib Noor Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sumaira Sharif
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Najm Us Saqib
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Sumaira Sharif or Najm Us Saqib.

Ethics declarations

Informed Consent

The research did not involve human subjects or personal information. No, informed consent is required.

Research Involving Human Participants and/or Animals

None.

Conflict of Interest

The authors declare no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Inam, M., Shah, A., Khan, W.N. et al. Biosynthesis of Silver Nanoparticles: Preparation, Optimization and In Vitro Anti-diabetic Effect. BioNanoSci. 11, 1154–1159 (2021). https://doi.org/10.1007/s12668-021-00895-6

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12668-021-00895-6

Keywords

Search

Navigation