Abstract
Hexane and ethyl acetate extracts of Clerodendrum phlomidis roots show antitubercular activity. Bioguided fractionation of active extracts led to the isolation of a new phenylethanoid glycoside, β-(4-hydroxyphenyl)-ethyl-O-α-l-rhamnopyranosyl(1 → 3)-β-D-(4-O-2′,6′-dihydroxycinnamoyl)-glucopyranoside (phlomidoside, 10) along with 12 known compounds. The structures of the isolated compounds were elucidated on the basis of detailed spectroscopic analysis (NMR, Mass and IR). All the compounds except 2, 4, and 8 have been isolated for the first time from C. phlomidis. Antitubercular activity of extracts and isolated compounds was determined by BACTEC radiometric susceptibility assay against Mycobacterium tuberculosis H37Rv (ATCC 27294). In addition, isolated compounds were also evaluated for their effect on lipopolysaccharide-stimulated macrophages for production of pro-inflammatory cytokines, tumor necrosis factor-α and interleukin-6. This is the first report on the antitubercular potential of C. phlomidis, indicating the underlying mechanism involved in the therapeutic application of a traditionally used plant.
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Acknowledgments
We are thankful to Director of the CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow for fundamental facilities for the research. We are also thankful to Director, National JALMA Institute for Leprosy and other Mycobacterial Diseases, Agra for providing Mycobacterium tuberculosis H37Rv.
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Yadav, A.K., Thakur, J.P., Agrawal, J. et al. Bioactive chemical constituents from the root of Clerodendrum phlomidis . Med Chem Res 24, 1112–1118 (2015). https://doi.org/10.1007/s00044-014-1191-x
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DOI: https://doi.org/10.1007/s00044-014-1191-x