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Insectes Sociaux

, Volume 60, Issue 4, pp 531–542 | Cite as

Nest architecture, fungus gardens, queen, males and larvae of the fungus-growing ant Mycetagroicus inflatus Brandão & Mayhé-Nunes

  • A. Jesovnik
  • J. Sosa-Calvo
  • C. T. Lopes
  • H. L. Vasconcelos
  • T. R. Schultz
Open Access
Research Article

Abstract

All known fungus-growing ants (tribe Attini) are obligately symbiotic with their cultivated fungi. The fungal cultivars of “lower” attine ants are facultative symbionts, capable of living apart from ants, whereas the fungal cultivars of “higher” attine ants, including leaf-cutting genera Atta and Acromyrmex, are highly specialized, obligate symbionts. Since higher attine ants and fungi are derived from lower attine ants and fungi, understanding the evolutionary transition from lower to higher attine agriculture requires understanding the historical sequence of change in both ants and fungi. The biology of the poorly known ant genus Mycetagroicus is of special interest in this regard because it occupies a phylogenetic position intermediate between lower and higher ant agriculture. Here, based on the excavations of four nests in Pará, Brazil, we report the first biological data for the recently described species Mycetagroicus inflatus, including the first descriptions of Mycetagroicus males and larvae. Like M. cerradensis, the only other species in the genus for which nesting biology is known, the garden chambers of M. inflatus are unusually deep and the garden is most likely relocated vertically in rainy and dry seasons. Due to the proximity of nests to the Araguaia River, it is likely that even the uppermost chambers and nest entrances of M. inflatus are submerged during the rainy season. Most remarkably, all three examined colonies of M. inflatus cultivate the same fungal species as their congener, M. cerradensis, over 1,000 km away, raising the possibility of long-term symbiont fidelity spanning speciation events within the genus.

Keywords

Mycetagroicus inflatus Ants Attini Fungus-growing ants Symbiont fidelity Nest architecture 

Introduction

The ant genus Mycetagroicus, described in 2001, is a member of the ant tribe Attini, all of which are obligate agriculturalists and fungivores. In establishing the genus, Brandão and Mayhé-Nunes (2001) included three species: M. cerradensis, M. triangularis, and M. urbanus. In 2008, the same authors described a fourth species, M. inflatus, based on two workers collected by R. Feitosa and R. Rosa da Silva in southern Pará, Brazil, in 2005 (Brandão and Mayhé-Nunes, 2008). The descriptions of three of the four Mycetagroicus species were based solely on workers. For M. triangularis, a single specimen of the gyne was described. Until now, Mycetagroicus males and larvae have remained entirely unknown.

Mycetagroicus occupies a critical position in the phylogeny of the Attini (Schultz and Brady, 2008). It is the sister group of the so-called higher Attini (a group containing the genera Trachymyrmex, Sericomyrmex, Acromyrmex, and Atta) which cultivate a clade of obligately symbiotic fungal species that share a lower attine fungal ancestor, all of which are highly specialized for symbiosis with their ant hosts (Mueller and Gerardo, 2002; Schultz et al., 2005; Mikheyev et al., 2006, 2007; Schultz and Brady, 2008). Based on worker morphology, Brandão and Mayhé-Nunes (2008) suggested that the genus might be a member of the higher Attini, but Solomon et al. (2011), based on a molecular analysis of the cultivar, found that M. cerradensis is in fact a member of the lower Attini, i.e., it is associated with a lower attine cultivar. The fungal cultivars of the other three Mycetagroicus species have until now remained unknown.

Since the genus is the sister group of the higher attines, study of Mycetagroicus species can potentially provide insights regarding the origin of higher attine agriculture, arguably the most ecologically significant transition in the evolutionary history of the Attini. During the past few decades, new natural history information has required periodic, sometimes dramatic revisions of our model of the complex co-evolutionary relationships among fungus-growing ants, their fungal cultivars, and other microbial symbionts (Chapela et al., 1994; Mueller et al., 1998; Currie et al., 1999; Mikheyev et al., 2006, 2007; Mehdiabadi and Schultz, 2010; Mueller et al., 2011a; Mehdiabadi et al., 2012).

Nest architecture has been studied from an evolutionary perspective in a number of different organisms, especially in birds, termites, and social Hymenoptera. In some studies, nest characters were included in a phylogenetic data matrix and analyzed along with other character data to produce a phylogeny (Lanyon, 1986; Wenzel, 1998; Zyskowski and Prum 1999), whereas in other studies, nest characters were mapped onto pre-existing phylogenies to test whether nest traits were correlated with phylogenies obtained from non-nest-related character information (Packer, 1991; Rasmussen and Camargo, 2008). A study of termites in the genus Apicotermes Holmgren, 1912 included a key to the species based solely on nest morphology (Schmidt, 1955), concluding that termite nests contain more phylogenetic information than the termites themselves. In ants, studies of underground nests (Tschinkel, 2003) and of nest entrance morphology (Schultz et al., 2002) have revealed patterns in structure and morphology that are species-specific. At deeper phylogenetic levels, however, due to convergence and parallelism nest architecture characters are often homoplastic, arising multiple times on the ant tree of life (Tschinkel, 2011). Nest architecture in fungus-growing ants is important not only for the ants but also for their fungal cultivars, which require particular environmental conditions (Bollazzi and Roces 2002; Bollazzi et al. 2008). Nest architecture, therefore, is a significant aspect of attine ant biology, and the importance of documenting attine nest life histories has been recognized by multiple researchers (Weber, 1972; Solomon et al., 2004, 2011; Moreira et al., 2004; Fernández-Marín et al. 2005; Diehl-Fleig and Diehl, 2007; Klingenberg et al., 2007; Rabeling et al., 2007; Mehdiabadi and Schultz, 2010; Ramos-Lacau et al., 2012).

In addition to describing nest architecture, we identify the fungal cultivar of Mycetagroicus inflatus. We describe the previously unknown queen, provide the first description of a male for the genus, and compare both to reproductive forms of other, closely related attine genera. We also describe for the first time the larva of a Mycetagroicus species.

Materials and methods

Field work was conducted from 5 to 10 October 2012 in eastern Pará State, Brazil, at the end of the dry season. During this time period the first rains of the rainy season occurred. All nests were found at two localities on the west coast of the Araguaia River, a southern component of the Amazon River Basin, across from the town of Araguacema, Tocantins, in an area characterized as “dense alluvial forest” (Silva, 2007). At “Locality 1” (S 08.75387°, W 49.54544°, elevation 164 m), nest entrances occurred approximately 15 m from the river’s edge in an area about 7 m above water level, which was at its seasonal low point; at “Locality 2” (S 08.80157°, W 49.57368°, elevation 176 m), they occurred approximately 5 m away from the river’s edge and about 5 m above the water level. At both localities the beach area is sandy, with occasional low bushes, and banks are covered with gallery forest. Based on information from local inhabitants, both Locality 1 and Locality 2 are entirely submerged during the rainy season (December–March).

In both localities, nests were located in the morning by following foraging workers returning with bait (Cream of Rice cereal) to their nest entrances. Nests were excavated using Gerber folding shovels by first digging a trench approximately 1.5 m away from the nest entrance (Rabeling et al., 2007; Solomon et al., 2011). The trench was extended by carefully shaving away soil with a shovel or a knife in the direction of the nest entrance. As the trench grew closer to the nest entrance, digging became increasingly careful in order to avoid missing or destroying chambers and tunnels. Since the depths at which M. inflatus constructs its deepest chambers were initially unknown, after locating a chamber excavation continued downward 0.5–1 m in order to locate possible additional chambers. If a chamber contained a queen, it was assumed that it was the bottom-most chamber and excavation ceased. When a chamber was located, part of the chamber wall near the excavator was removed and the fungus garden, when present, was transferred, using a flame-sterilized forceps, spoon, and/or knife, to a plastic nest box with a plaster bottom pre-saturated with water. Ants were collected with sterilized soft forceps and with an aspirator. Chamber dimensions were recorded, including: height (maximum length along vertical axis), width (maximum length along horizontal axis parallel to the excavation plane), depth (maximum length along horizontal axis perpendicular to the excavation plane), and distance from the surface to the chamber roof (summarized in Table 1). Nests were maintained in live laboratory culture and were still alive in the lab at the time of this writing (July 2013). Several workers were preserved immediately in 95 % ethanol for later DNA extraction. A few days following collection, small portions of fungus gardens were collected from the live lab nests and likewise preserved in 95 % ethanol. The time interval between field collections of the nests and preservation of garden samples allowed the ants to reassemble the gardens, which are usually broken apart during collection, and to remove particles of soil, which are usually introduced during nest excavation. Estimated number of individuals for each colony is based on the count of the workers in the laboratory nests; it may be an underestimate because laboratory colonies appear to have fungus gardens that are smaller than those initially collected in the field.
Table 1

Summary of nest data

Nest ID

 

Chamber 1

Chamber 2

Chamber 3

Chamber 4

JSC121006-02 Locality 1

Depth

80 cm

94 cm

103 cm

\

Dimensions

6.5 × 4 × 5 cm

5 × 4 × 7 cm

7.5 × 6 × 7 cm

 

Contents

fn, w, al

fn, w, al

fn, w, al, q

 

TRS121006-07 Locality 1

Depth

22 cm

68 cm

83 cm

96 cm

Dimensions

4 cm

2 cm

7 cm

7 × 8 cm

Contents

Loose sand

w, m

w, al

fn, w, g, m, 2q

TRS121009-01 Locality 2

Depth

87 cm

100 cm

230 cm

\

Dimensions

8 × 4 × 10 cm

8 × 5 × 9 cm

3 × 3 × 3.5 cm

 

Contents

w

fn, 1q?

1q?

 

TRS121009-02 Locality 2

Depth

75 cm

310 cm

\

\

Dimensions

9 × 5 × 12 cm

7 × 4 × 7 cm

  

Contents

fn, w

fn, w, m

  

Dimensions: width × height× depth

fn fungus garden, w workers, q queen, m males, g alate females, al alates (sex not recorded); for nest TRS121009-01, 1q? indicates that we did not record in which chamber the queen was found

In the lab, a few strands of hyphae from 3 different nests (collection codes JSC121006-02, TRS121006-07 and TRS121007-01) were separated from the field-preserved garden material with flame-sterilized forceps under a stereomicroscope and DNA was extracted from them using the Chelex protocol of Sen et al. (2010). The extracted DNA was amplified and sequenced for the nuclear ITS region following the methods of Mueller et al. (1998). This DNA sequence was incorporated into a large alignment (>440 sequences) of fungal cultivars and free-living Leucocoprineae and aligned using MAFFT (Katoh and Standley, 2013) using parameter values E-INS-i, 200PAM/k = 2, and Gap open penalty = 1.2. The sequences are deposited in GenBank under accession numbers KF562343, KF562444, and KF562345.

In the laboratory, ant specimens were examined and measured using SZH Olympus and MZ16 Leica stereomicroscopes. Specimens were photographed using a JVC KY-F70B video camera with M420 Leica stereomicroscope and Automontage Pro version 5.03.0018 software. Larval morphology was examined with scanning electron microscopy (SEM). Five specimens were studied, including three last-instar worker larvae and two worker prepupae (i.e., post-feeding last instars), all taken from nest collection TRS121006-07. Morphological terminology and measurement indices follow Snodgrass (1910), Tulloch (1935), Gauld and Bolton (1988), Hölldobler and Wilson (1990), Bolton (1994), Schultz and Meier (1995), Mackay et al. (2004), Klingenberg and Brandão (2009), and Sosa-Calvo and Schultz (2010). When terminologies disagree, synonymous terms are indicated in parentheses. Morphological measurements, index abbreviations, and definitions are as follows: head width (HW): in frontal view, maximum width of the head just above eyes, excluding eyes; head width in males (HWm): in frontal view, maximum width of the head including eyes; interocular distance (IOD): in the male, maximum width of the head in frontal view measured at the midpoint of the internal margin of eyes; head length (HL): in frontal view, maximum length of the head from posteriormost margin of the head to median point of anteriormost margin of clypeal apron; scape length (SL): maximum length of scape excluding the basal condyle; interfrontal width (IFW): maximum distance between lateral margins of frontal lobes; mandible length (ML): in frontal view, straight-line length from midpoint of anterior margin of clypeal apron to tip of mandibles when fully closed; eye length (EL): in profile, maximum diameter of eye; Weber length (WL): in lateral view, length of mesosoma from anteriormost point of pronotum, to posteriormost ventral angle of propodeum; hind femur length (HfL): length of hind femur in lateral view; petiole length (PL): in lateral view, maximum length of petiole; postpetiole length (PPL): in lateral view, maximum length of postpetiole; gaster length (GL): in lateral view, distance from anteriormost point of tergo-sternal gaster suture to the posterior tip; cephalic index (CI): for females (HW/HL) × 100, for males (IOD/HL) × 100; frontal lobes index (FLI): for females (IFW/HW) × 100, for males (IFW/IOD) × 100; scape index (SI): for females (SL/HW) × 100, for males (SL/IOD) × 100.

Deposition of material: The specimens examined are deposited in USNM, National Museum of Natural History, Washington, DC, USA; MZSP, Museu de Zoologia da Universidade de São Paulo, São Paulo, SP, Brazil; MBC–UFU, Museu de Biodiversidade do Cerrado, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil; and MCZ, Museum of Comparative Zoology, Harvard University, Cambridge, MA, USA.

Results

Nest architecture

Four nests of Mycetagroicus inflatus were excavated. All nests had a single, simple, very inconspicuous entrance hole approximately 2–3 mm in diameter. In no case was the nest entrance surrounded by a mound of excavated soil particles, differing in this regard from entrances of the congeneric M. cerradensis (Solomon et al., 2011). Nests contained 2–4 chambers, which varied from 2–8 cm in width. The deepest chamber encountered was 310 cm deep and contained garden and ants, but no queen. The shallowest chamber encountered was 22 cm deep and contained no fungus or ants, only loose sand. The shallowest chamber that contained ants was 68 cm deep, and the shallowest chamber that contained fungus garden was 75 cm deep. Tunnels connecting chambers were observed in some cases and were very straight and perpendicular to the surface (Fig. 1). The walls of two nest chambers, from nests TRS121009-01 and -02, were punctuated with multiple holes (Fig. 1c). Colony sizes ranged from approximately 30–100 workers.
Fig. 1

Mycetagroicus inflatus nest: a Illustration of nest TRS121006-07. b Photo of excavated nest JSC121006-02. c Photo of chamber 1 of nest TRS121009-02 showing the many holes punctuating the chamber wall

One chamber of a fifth nest was encountered accidentally (TRS121007-01) while digging, but no data on the position of the nest entrance or other chambers were available for that nest, so it is not treated as a separately excavated nest in Table 1. This isolated chamber was encountered at 80 cm depth, was 6 cm wide, and contained a fungus garden, queen, workers, and brood.

Fungal symbiont

ITS sequences indicate that the fungal cultivars collected from three nests of M. inflatus (JSC121006-02, TRS121006-07, and TRS121007-01) all belong to subclade F of the so-called “Clade 2” of lower attine (G3) leucocoprineaceous cultivars (Mehdiabadi et al., 2012; Mueller et al., 1998). Subclade F is arguably a single fungal species that is also associated with Cyphomyrmex faunulus, Mycocepurus smithii, Myrmicocrypta cf. buenzlii, and other attine species from Ecuador, Trinidad, Guyana, and Brazil (Mehdiabadi et al., 2012; unpubl. data). Remarkably, the fungal species cultivated by M. inflatus in Pará is also grown by its congener Mycetagroicus cerradensis (Solomon et al., 2011, accession number in GenBank HM245775) over 1,000 km to the south in Minas Gerais.

Arthropod symbiont

While following a M. inflatus forager carrying Cream of Rice bait back to its nest, a fly, later identified as belonging to the genus Pholeomyia Bilimek (Milichiidae: Diptera), was observed following the worker by walking a short distance behind it (JSC, pers. obs.). Sabrosky (1959) reported similar observations by W.L. Brown and E.O. Wilson of a Pholeomyia fly following a worker of the fungus-growing ant Trachymyrmex septentrionalis McCook in Florida and suggested a possible association of certain Pholeomyia species with fungus-growing ants. Later, Waller (1980) reported finding Pholeomyia texensis Sabrosky entering the nests of Atta texana by riding on the cut leaves, where the fly larvae presumably feed on nest refuse. Larvae of some species of milichiids have close associations with Hymenoptera (Sabrosky, 1959; Krombein, 1967; Moser and Neff 1971; Melo, 1996; Wild and Brake, 2009; Swann, 2010).

Systematic treatment

Mycetagroicus inflatus, Brandão & Mayhé-Nunes 2008

Gyne (Fig. 2)
Fig. 2

Mycetagroicus inflatus gyne: a Full-face view, b dorsal view, c lateral profile, d fore- and hindwing, e dorsal view of postpetiole

Measurements in mm (range):

HW 0.60–0.72, HL 0.72–0.76, ScL 0.62–0.66, IFW 0.38–0.39, ML 0.20–0.26, EL 0.15–0.17, WL 1.0–1.1, HfL 0.79–0.87, PL 0.18–0.23, PPL 0.32–0.37, GL 0.89–1.14, CI 84–94, FLI 55–62, SI 91–102 (n = 7).

Posterolateral corners of head, mesosoma, petiole, and postpetiole light ferruginous brown; gaster dark ferruginous brown. Entire dorsum of head in full-face view fuscous, darker than gaster. Mandibles, antennae, and anterior borders of frontal lobes distinctly lighter than the rest of the dorsum of the head. Integument finely reticulate-punctuate, opaque. Erect hairs absent, most of the body covered with short, white, appressed hairs.

Head. In full-face view slightly longer than broad (CI 84–94), posterior corners of cephalic margin rounded, slightly notched medially. Mandibles triangular, dorsally striate, masticatory (inner) margin of mandible with 5 teeth, the distal-most tooth noticeably prolonged and larger than the rest. Outer margin of mandible straight. Margin of anterior clypeal apron convex, with a very broad and shallow but distinct median notch. Median clypeal seta (~0.15 mm) arising from border of clypeal apron and clypeal anterior margin, flanked on either side by a pair of additional, long setae as well as 2–3 shorter setae. Distinct frontoclypeal teeth present (“triangular lateral tooth” of Brandão and Mayhé-Nunes, 2008), best seen in lateral view. Frontal lobes evenly rounded, separated by a fingerlike extension of the clypeus extending posterad to the level of the eyes. Frontal carinae short, diverging toward corners of the head, not extending beyond median ocellus. Preocular carina distinct, extending posterad from mandibular insertion, then curving medially above eye level. Area laterad of frontal lobes, the ill-defined antennal scrobe, devoid of hairs. Eyes convex, 13 ommatidia across largest diameter of dorso-ventral axis and 10 ommatidia across smallest diameter. Three ocelli present, all similar in size. Antennal scape extending only slightly (~0.02 mm) past posterior border of head. Scape noticeably curved ventrad in basal one third, best seen in posterior view with scape positioned at 90° from median axis. Antenna 11-segmented, lacking distinctive antennal club. First funicular segment and segments 8–10 longer than broad, segments in between almost subquadrate. Palp formula 4, 2.

Mesosoma. Lateral pronotal tubercle present, dentiform. Anterior pronotal tubercle absent (“paired median pronotal teeth” of Brandão and Mayhé-Nunes, 2008). Antero-inferior corner of pronotum almost forming a right angle, best seen in lateral view. Anapleural suture present (“median episternal groove” of Snodgrass, 1910). Scutum lacking notauli or other sutures, shallowly impressed longitudinally. Parapsidal furrow absent. Axillae (“anterior division of scutellum” sensu Snodgrass, 1910; “prescutellum” sensu Tulloch, 1935; “paraptera” sensu Brandão and Mayhé-Nunes, 2008) relatively large, laterally rounded and medially constricted. Scutellum mostly flat in profile view, weakly concave and narrowing posteriorly in dorsal view, posterior border medially emarginate, not forming scutellar processes. Propodeal teeth short, blunt, and directed posterolaterally. Propodeal carinae absent. Propodeal spiracle directed posterad, mounted on a tumulus, best seen in dorsal view. Propodeal lobe lamellate, connected by a short, longitudinal carina, along bulla of metathoracic gland, to the dorsum of the propodeal spiracle, best seen in dorsolateral view.

Wings. Transparent, covered with minute pilosity, veins brown. Forewing (length: 3.62 mm) with 5 closed cells (terminology follows Goulet and Huber, 1993): costal (C), radial (R), cubital (Cu), first radial 1 (1R1), and first radial 2 (2R1). Pterostigma not visible. Hindwing (length: 2.57 mm) with reduced venation, just one closed cell, and 6 hamuli.

Metasoma. Petiole compact, relatively small, petiolar peduncle vestigial. Subpetiolar process present anteriorly, pointing forward, sometimes not clearly visible because concealed by propodeal lobes. From ventral view clearly visible as a spine. Petiole dorsum with two small denticles near its posterior border. Postpetiole large, >1.5× the width of the petiole, slightly broader than long in dorsal view. Posterior border of postpetiole wider than the rest of petiole, bearing protruding transverse plate that is rounded laterally and slightly emarginated medially (Fig. 2e). In dorsal view, gaster elliptical, narrowing posteriorly, lacking ridges or noticeable sculpture, but having finely punctuated microsculpture. Rows of long, suberect hairs present on posterior borders of gastral sternites 2–4 (A5–A7). Gastral tergite and sternite I subequal in length, the posteriormost tip of the gaster formed by segment A7 in contrast to the (presumably derived) condition in some Attini, in which gastral tergite I longer than sternite I so that posteriorly it overlaps the remaining segments, which are shifted anterad.

Differential diagnosis. The gyne of M. inflatus is, as in many ant species, quite similar to the worker except for modifications typical for the caste such as the presence of ocelli, the morphology of the mesosoma associated with wings, and the slightly larger size. In addition, in the worker the eyes are smaller, the frontoclypeal teeth are less pronounced, the frontal lobes are narrower, median anterior tubercles are present on the pronotum, and the petiole is shorter. The gyne of M. inflatus can be distinguished from the gynes of other closely related attine genera by characters that are synapomorphic for the genera. Sericomyrmex gynes, for instance, are larger in size and covered with dense pilosity, have cordate heads, have gasters that are laterally straight instead of rounded, and have frontal lobes that are more developed than those in Mycetagroicus. Gynes of Trachymyrmex species are in most cases larger in size and have integuments that are moderately to strongly tuberculate, frequently appearing spiny, most noticeably on the posterior lobes of head and on the gaster, which in M. inflatus are smooth. Cyphomyrmex gynes can be similar in size to gynes of M. inflatus, but are immediately distinguished by the strongly expanded frontal lobes, often covering a large portion of the head, and in the strigatus and wheeleri groups accompanied by deep antennal scrobes. The gyne of the congeneric M. triangularis can be distinguished from that of M. inflatus by its slightly larger size, the presence of a median longitudinal ridge posterior to the frontal triangle, the presence of a distinct parapsidal furrow, the presence of scutellar processes, and the rugulose microsculpture on the dorsum of first gastral segment (A4), which also bears two pronounced ridges laterally.

Male (Fig. 3)
Fig. 3

Male of Mycetagroicus inflatus: a full-face view, b lateral profile, c dorsal view, d fore- and hindwings

Measurements in mm (range):

HWm 0.53–0.61, IOD 0.37–0.40, HL 0.45–0.56, ScL 0.44–0.62, IFW 0.15 0.19, ML 0.14–0.21, EL 0.13–0.19, WL 0.90–1.16, HfL 1.05–1.35, PL 0.14–0.20, PPL 0.21–0.29, GL 0.80–1.07, CI 77–81, FLI 41–44, SI 120–142 (n = 7).

Body color dark brown, integument reticulate-punctuate on head and mesosoma, gaster opaque with reticulate microsculpture. Erect hairs absent, short appressed white hairs covering head, dorsal side of mesosoma, gaster, and legs; only a few hairs present on lateral side of mesosoma.

Head. In full-face view longer than broad, posterior border smoothly rounded. Mandibles triangular, dorsally striate, basal angle rounded, masticatory margin with three teeth, all arising in the distal half, an unusual arrangement shared with males of Trachymyrmex urichi and perhaps other Trachymyrmex species. Clypeus evenly reticulate, without frontoclypeal carinae or teeth as seen in worker and queen. Anterior margin of clypeal apron convex, with shallow median notch. Median clypeal seta (~0.12 mm) arising at the point where posterior margin of clypeal apron and anterior clypeal margin meet flanked by one much shorter seta on each side. Frontal lobes small, short, not completely covering the antennal condyle, broadly separated by a fingerlike extension of the clypeus extending posterad. Frontal carinae short, extending to the level of the posterior border of the eyes. Preocular carina distinct, curving posteriorly toward the midline. Eyes convex, large, 16 ommatidia across largest diameter of dorso-ventral axis, 15 ommatidia across the smallest diameter of the eye. Three similarly sized ocelli present. Antennal scape straight, long, extending well beyond the occiput. Antenna 12-segmented, a departure from the plesiomorphic number of 13 for ant males (including attine males), the first funicular segment (pedicel) as long as second funicular segment, or slightly longer. First funicular segment longer than broad and thicker than the second funicular segment, but not thicker than the most distal segments. Palp formula 4, 2.

Mesosoma. Lateral pronotal tubercles absent. Antero-inferior corner of pronotum rounded. Anapleural suture present, dividing mesopleuron into katepisternum and anepisternum. A wide, transversely costate groove present on dorsoposterior border of anepisternum below insertion of wing. In some males the anapleural suture can also be transversely costate. Scutum with shallow, complete, V-shaped notauli, dividing the scutum into an anteromedian area (“prescutum”) and two lateral areas. Median mesoscutal sulcus present, fading posteriorly. Parapsidal lines present. Axillae relatively large, rounded laterally, constricted medially. Scutellum slightly inflated, narrowing posteriorly, posterior border with two short, blunt denticles. Propodeal teeth reduced to short, blunt denticles.

Wings. Forewing (length: 3.81 mm), hindwing (length: 2.7 mm). Venation and appearance same as in female, except in male spur of cross vein 1 m-cu is visible protruding from the bottom of 1R1 cell and 7 hamuli instead of 6 on hind wing.

Metasoma. Petiole compact, node in dorsal view rounded, slightly broader than long. Petiolar sternite narrowing to a forward directed, sharp keel. Postpetiole in dorsal view rounded, slightly broader than long, posteriorly emarginate. Integument of tergum 1 of gaster (i.e., A4) more shining than the other body parts, but still subopaque due to the finely reticulate microsculpture; bearing sparse, white, appressed hairs. In dorsal view, gaster elliptical, narrowing posteriorly, without any carinae or other macrosculpture. Gastral tergite and sternite I subequal in length and gastral tergites 2–5 (i.e., A5–A8) visible dorsally or posterodorsally. Rows of long, suberect hairs present on posterior borders of gastral sternites 2–5 (A5–A8).

Differential diagnosis. Males of M. inflatus have 12-segmented antennae, a deviation from the usual 13-segmented antennae present in the males of most attine species, although the 12-segmented condition has arisen independently elsewhere in the tribe in the genus Sericomyrmex, in some Cyphomyrmex species (e.g., C. faunulus and C. auritus), in Trachymyrmex opulentus, and in a number of social parasites in the genus Acromyrmex (Gallardo, 1916). Reductions in male antennal segment number are even more dramatic in males of the socially parasitic attine species Mycocepurus castrator (Rabeling and Bacci, 2010) and Pseudoatta argentina (Gallardo, 1916; Schultz et al., 1998), which have 11-segmented antennae. The male of M. inflatus can be distinguished from males of closely related genera by the following combination of characters: presence of 12 antennal segments, reticulate-punctuate integument (even on the clypeus), dark brown color, transversely costate groove on the mesopleuron, and propodeal teeth very reduced. Males of Sericomyrmex can be distinguished from males of M. inflatus by the presence of dense appressed pilosity covering the whole body and longer, thicker, decumbent to erect hairs on the head, mesosoma, and dorsum of the gaster. Males of Trachymyrmex usually also have denser pilosity, are sometimes covered with spiny tubercules on the head and gaster, and usually have sharp propodeal spines and humeral tubercles. Males of the closely related species Cyphomyrmex costatus and C. wheeleri have 13 antennal segments, strongly developed frontal lobes, antennal scrobes that reach the posterior border of the head, and sharp propodeal spines.

Larva (Fig. 4)
Fig. 4

Larvae and prepupae of Mycetagroicus inflatus: a lateral profile (prepupae), b head (prepupae), c head, lateral view (prepupae), d mouthparts (last-instar larvae)

Specimens examined: three last-instar worker larvae and two worker prepupae (i.e., post-feeding last instars), all from nest collection TRS121006-07. Profile “attoid” sensu Wheeler and Wheeler 1976, i.e., with a moderately curved, ventrally shortened profile. As in all other Attini, thoracic-abdominal articulation absent, thoracic intersegmental constrictions superficial, deep lateral depressions associated with abdominal spiracles absent, and leg vestiges present as open slits. As in most other Attini, dorsal and lateral body surfaces devoid of setae. Differing from other Attini in the remarkably small number of head and ventral setae. Head devoid of setae except for two setae on each gena, a state previously unknown in attine larvae; ventral thoracic segments 1–3 each with a single pair of setae laterally; and two short setae on abdominal segment 10 ventral to the anus. Ventral thoracic segments 1 and 2 medially bearing multidentate spinules, thoracic segment 2 additionally with a low ventromedian boss, in combination with the genal and thoracic setae clearly functioning as a food anchor. As in most Neoattini, genal lobes present. Labrum monolobate, narrow, bulging, a synapomorphy for the Attini; anterior labral setae reduced to papillae. Mandibles typically attine: short, fleshy, subconical. A distinct, undivided apical mandibular tooth and no subapical teeth; spinules sparsely but evenly distributed on all mandibular surfaces. Mandibular gnathobases absent. Basal portions of maxillae fused with head capsule. As in all other Neoattini, maxillary palp widely removed laterad from galea. Galea reduced, present as two sensilla surmounting a low welt; a shallow pit distal and immediately adjacent to the galea. Maxillary palp digitiform, maxillary accessory palpal sensillum absent. One seta on each maxilla between the galea and palp, a second seta present laterad of the palp. As in most attines, labium feebly protruding and lateral sericteral protuberances absent; labial palps papilliform. Labial spinules present basad and absent distad of the sericteries. Hypopharyngeal spinules largely unidentate and moderately to sparsely distributed.

Discussion

The genus Mycetagroicus is the sister group of the higher Attini (Sericomyrmex, Trachymyrmex, Acromyrmex, and Atta) (Schultz and Brady, 2008). Thus, understanding the biology of Mycetagroicus is our best hope for reconstructing the attributes of the most recent common ancestor that it shares with the higher Attini, especially when considered in combination with the biology of the most closely related outgroup [currently thought to be the Cyphomyrmex wheeleri clade (Schultz and Brady, 2008)]. Reconstructing the common ancestor of Mycetagroicus + the higher Attini is important for reconstructing the evolutionary transitions that occurred on the branch subtending the higher Attini, arguably the most ecologically significant period of evolutionary change in all of the Attini. These transitions coincide with the origin of the obligate higher attine fungal symbiont, derived from a lower attine fungal species (Schultz et al., 2005; Mikheyev et al., 2006, 2007; Mueller et al., 2010; Mueller et al., 2011a). This study adds to the scant biological information so far accumulated for the four known Mycetagroicus species. It describes the gyne of M. inflatus, increasing to two the numbers of species for which gynes are known, the other being M. triangularis (Brandão and Mayhé-Nunes, 2001). It describes for the first time Mycetagroicus males and larvae. The nest architecture of M. inflatus is also described, increasing to two the number of species for which nest architecture is known, the other being M. cerradensis (Solomon et al., 2011).

All four excavated nests of Mycetagroicus inflatus had similar characteristics. The walls of one chamber in each of the two nests from Locality 2 (TRS121009-01: chamber depth not recorded; TRS121009-02: chamber 75 cm deep) were punctured by numerous holes of unknown purpose (Fig. 1c). It is possible that such punctures were present in additional chambers but were overlooked. It is highly unlikely that all of the holes were the openings of tunnels because no ants were observed entering or exiting and, if they were tunnel openings, they would connect with dozens of horizontal tunnels whereas no such tunnels were encountered; all of the tunnels observed during excavation were vertically arranged. One possibility is that the holes are somehow associated with flooding, perhaps serving as shelters for the ants during the months that the nests are submerged or serving in some way to capture and hold pockets of air. This conjecture gains support from the observation that similarly punctured chamber walls, as described above, were observed in the deepest chambers of colonies of the sympatric but very distantly related species Kalathomyrmex emeryi, colonies of which are certainly flooded during the rainy season. In addition to being punctured by numerous holes, unlike those of M. inflatus the chamber walls of K. emeryi were lined with a layer of brown clay.

The nest architecture of Mycetagroicus cerradensis, the only other congeneric species for which nesting biology is known, shares some characteristics with that of M. inflatus, including: a single nest entrance, chambers that are more or less vertically arranged below the nest entrance, and an extremely deep lowermost garden chamber (Solomon et al., 2011). Solomon et al. (2011) suggested that M. cerradensis moves its fungus garden seasonally, using deeper chambers in the dry season and shallower chambers in the wet season. This hypothesis applies equally to M. inflatus because, in both species, nests were excavated in the dry season and the uppermost nest chambers were found to be empty. Seasonal repositioning of fungus gardens has been recorded for some other fungus-growing ants, including two species of the genus Mycocepurus (Rabeling et al., 2007) and in the North American species Atta texana and Acromyrmex versicolor (Moser, 1962, 2006; Mueller et al., 2011a, b).

Perhaps the most potentially important result of this study is the identification of the fungal cultivars grown by M. inflatus at the study sites on the Araguaia River. The gardens from all three collected nests belong to the same fungal species (Clade 2, subclade F; Mehdiabadi et al., 2012), suggesting that M. inflatus may depart from most lower attine species in possessing a high degree of symbiont fidelity. Even more surprising, M. inflatus cultivates the same fungal species as its congener M. cerradensis in Minas Gerais, Brazil, over 1000 km to the south (Solomon et al., 2011). Field research during the past two decades has established that attine cultivars are frequently transmitted horizontally between colonies, including colonies of different ant species, and that free-living fungi are frequently imported into the lower attine symbiosis as well (Mueller et al., 1998; Vo et al., 2009; Green et al., 2002; Mueller et al., 2001; Mikheyev et al., 2008). These observations have led to the current paradigm of “diffuse” attine ant/fungus coevolution (Mikheyev et al., 2006; Mikheyev et al., 2007; Mikheyev et al., 2010) and a general expectation of weak symbiont fidelity and frequent reassociations of ants and fungi in attine agriculture. One exception to this rule is the Cyphomyrmex wheeleri group, in which it has recently been shown that particular ant and fungal species have been associated for many millions of years (Mehdiabadi et al., 2012). Mycetagroicus may represent a second such exception for reasons mentioned above. Interestingly, the genus Mycetagroicus is the sister group to the higher Attini and the C. wheeleri group is thought to be the sister group to the clade containing Mycetagroicus and the higher Attini, suggesting that an increase in symbiont fidelity may have preceded the origin of the higher Attini. Although based on only four samples, this pattern is consistent with a scenario of strong symbiont fidelity that spans speciation events. Obviously, additional samples are necessary for judging whether this pattern of symbiont fidelity continues to hold or whether the observed association with the same fungal species within and across species of Mycetagroicus is due to a rare coincidence.

Symbiont choice is favored when potential symbionts vary in their abilities to enhance partner fitness. Mycetagroicus species live in habitats that may be at least seasonally inhospitable to free-living leucocoprineaceous fungi. Three of the four species in the genus Mycetagroicus are found in the Cerrado; the fourth, M. inflatus, is so far known from seasonally flooded riverbanks in a region characterized as “dense alluvial forest” (Silva, 2007). In such habitats, it may be the case that some fungal symbionts are better adapted than others, providing conditions that favor symbiont choice and long-term, close associations of particular ant and fungal species. Arguing against this, however, is the observation that most Cyphomyrmex wheeleri group ant species live in wet forests where feral leucocoprineaceous cultivars are known to commonly occur (Mueller et al., 1998) and in which nests of different ant species, each containing its particular fungal symbiont species, often co-occur within centimeters of one another (Schultz et al., 2002). Clearly, more data are required for understanding the mechanisms that produced the genetic and morphological modifications encountered today in the spectacularly successful higher attine fungi and ants.

Notes

Acknowledgments

We thank the Brazilian Council of Research and Scientific Development for granting permission to conduct this research in Brazil (Processo CNPq 001884/2012-3). We also thank the Instituto Chico Mendes de Conservação da Biodiversidade (ICMBio) for issuing collecting permit number 14789-6. We are grateful to the discoverers of Mycetagroicus inflatus, Rodrigo Feitosa and Rogerio Rosa da Silva, for their help and advice, which were essential to relocating nests of M. inflatus on the banks of the Araguaia River. We are grateful to two anonymous reviewers for greatly improving the manuscript. Thanks to Eliana Buenaventura (University of Copenhagen) for identifying the milichiid fly, to Michael Lloyd for molecular bench work, and to Eugenia Okonski for specimen preparation. Travel expenses for AJ were partly supported by an Explorers Club Washington Group Exploration and Field Research Grant awarded in 2012. TRS, AJ, and JSC were supported by NSF DEB 0949689 and TRS by a Smithsonian Institution National Museum of Natural History Small Grant Award. JSC was additionally supported by a NMNH Peter S. Buck graduate fellowship.

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Copyright information

© The Author(s) 2013

Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

Authors and Affiliations

  • A. Jesovnik
    • 1
    • 2
  • J. Sosa-Calvo
    • 1
    • 2
  • C. T. Lopes
    • 3
  • H. L. Vasconcelos
    • 3
  • T. R. Schultz
    • 2
  1. 1.Department of Entomology, Maryland Center for Systematic EntomologyUniversity of MarylandCollege ParkUSA
  2. 2.Department of EntomologyNational Museum of Natural History, Smithsonian InstitutionWashingtonUSA
  3. 3.Instituto de BiologiaUniversidade Federal de UberlândiaUberlândiaBrazil

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