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Exosomes from TNF-α preconditioned human umbilical cord mesenchymal stromal cells inhibit the autophagy of acinar cells of severe acute pancreatitis via shuttling bioactive metabolites

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Abstract

Severe acute pancreatitis (SAP) is a common critical disease of the digestive system, with high mortality and a lack of effective prevention and treatment measures. Despite mesenchymal stromal cell transplantation having the potential to treat SAP, its clinical application prospect is limited, and the mechanism is unclear. Here, we reveal the therapeutic role of exosomes from TNF-α-preconditioned human umbilical cord mesenchymal stromal cells (HUCMSCs) in attenuating SAP and show that it is partly dependent on exosomal metabolites. Bioactive metabolomics analysis showed that 48 metabolites be significantly differentially expressed between the two groups (Exo-Ctrl group versus Exo-TNF-α group). Then, the further functional experiments indicated that 3,4-dihydroxyphenylglycol could be a key molecule mediating the therapeutic effect of TNF-α-preconditioned HUCMSCs. The animal experiments showed that 3,4-dihydroxyphenylglycol reduced inflammation and oxidative stress in the pancreatic tissue and inhibited acinar cell autophagy in a rat model of SAP. Mechanistically, we revealed that 3,4-dihydroxyphenylglycol activated the mTOR pathway to inhibit acinar cell autophagy and alleviate SAP. In summary, our study demonstrated that exosomes from TNF-α-preconditioned HUMSCs inhibit the autophagy of acinar cells of SAP by shuttling 3,4-dihydroxyphenylglycol and inhibiting the mTOR pathway. This study revealed the vital role and therapeutic potential of metabolite-derived exosomes in SAP, providing a new promising method to prevent and therapy SAP.

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Availability of data and materials

The data generated in this study are available upon request from the corresponding author.

Abbreviations

SAP:

Severe acute pancreatitis

AP:

Pancreatitis

HUCMSCs:

Human umbilical cord mesenchymal stromal cells

MDT:

Multidisciplinary team

MSCs:

Mesenchymal stromal cells

EVs:

Extracellular vesicles

FBS:

Fetal bovine serum

TEM:

Transmission electron microscopy

NTA:

Nanoparticle tracking analysis

ROS:

Reactive oxygen species

MDA:

Malondialdehyde

SOD:

Superoxide dismutase

CAT:

Catalase

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Acknowledgements

We thank Prof. Xingyun Wang from Shanghai Jiao Tong University School of Medicine for his support and guidance on Metabonomic analysis.

Funding

This work was supported by the National Natural Science Foundation of China (82200717 and 81670582).

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Author notes

  1. Zhilong Ma and Wangcheng Xie contributed equally to this work.

Authors and Affiliations

  1. Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, No. 270 Dong’An Road, Shanghai, 200032, China

    Zhilong Ma, Jie Hua, Wei Wang, Xianjun Yu, Jin Xu & Si Shi

  2. Department of General Surgery, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, 200072, China

    Zhilong Ma, Wangcheng Xie, Tingyi Luo, Zhengyu Hu, Jia Zhou, Tingsong Yang & Zhenshun Song

  3. Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200050, China

    Zhilong Ma & Jia Zhou

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  1. Zhilong Ma
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Contributions

All authors contributed to the study conception and design. ZM, WX, TL, ZH, and JH performed the experiments and participated in drafting the manuscript. XY, JZ, WW, TY, and ZS performed conception design, data collection, and analysis. SS and JX performed study design, data interpretation, and supervised the review. All authors have read and approved the article.

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Correspondence to Jin Xu or Si Shi.

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The animal study was approved by the Ethics Committee of Shanghai Tenth People’s Hospital, Tongji University School of Medicine (SHDSYY-2022-2330).

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Ma, Z., Xie, W., Luo, T. et al. Exosomes from TNF-α preconditioned human umbilical cord mesenchymal stromal cells inhibit the autophagy of acinar cells of severe acute pancreatitis via shuttling bioactive metabolites. Cell. Mol. Life Sci. 80, 257 (2023). https://doi.org/10.1007/s00018-023-04861-1

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  • DOI: https://doi.org/10.1007/s00018-023-04861-1

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