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Enhancing or inhibiting apoptosis? The effects of ucMSC-Ex in the treatment of different degrees of traumatic pancreatitis

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Abstract

The animal models of traumatic pancreatitis (TP) were established to evaluate the specific mechanisms by which umbilical cord mesenchymal stem cell-derived exosomes (ucMSC-Ex) exert therapeutic effects. Sixty four rats were randomly divided into eight groups, including TP groups with three different degrees and relevant groups with ucMSC-Ex treated. The degrees of pancreatic tissue injury were evaluated by Histological Examination. Furthermore, enzyme-linked immunosorbent assay were applied to evaluate the activity of pancreatic enzymes and levels of inflammatory factors in serum. Finally, the apoptotic effects of each group were evaluated by TUNEL, western blot (WB), and real time fluorescence quantitative polymerase chain reaction (RT-qPCR). The pancreatic histopathological score and serum amylase and lipase levels gradually increased in various degrees of TP and the levels in the treatment group were all significantly decreased. The apoptosis index gradually increased in each TP group and significantly decreased in the treatment group in TUNEL results. WB and RT-qPCR showed the same trend, that bax and caspase-3 gradually increased and bcl-2 gradually decreased in TP groups. Compared with TP groups, the expression of bax and caspase-3 were lower while bcl-2 expression was higher in the treatment group. ucMSC-Ex suppressed the inflammatory response and inhibited pancreatic acinar cell apoptosis to promote repair of injured pancreatic tissue.

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Acknowledgements

Not applicable.

Funding

Hospital Management of the General Hospital of Western Theater Command (2021-XZYG-B16), the Sichuan Science and Technology Program (2022YFS0195).

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Author notes

  1. Zhao Zhirong and Han Li have contributed equally to this work.

Authors and Affiliations

  1. General Surgery Center, General Hospital of Western Theater Command, No. 270, Rongdu Rd, Jinniu District, Chengdu, 610083, Sichuan, China

    Han Li, Zhou Lichen, Tan Zhen, Wang Tao & Dai Ruiwu

  2. College of Medicine, Southwest Jiaotong University, Chengdu, China

    Zhao Zhirong, Han Li & Dai Ruiwu

  3. The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, China

    He Yiqun

  4. Hyperbaric Oxygen Department, General Hospital of Western Theater Command, Chengdu, Sichuan, China

    He Chunyang

  5. College of Clinical Medicine, Southwest Medical University, Luzhou, Sichuan, China

    Zhou Lichen & Dai Ruiwu

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  1. Zhao Zhirong
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Contributions

ZRZ and LH conceived and implemented the experiments; YQH, CYH, LCZ and ZT conducted the statistical analysis; TW revised the manuscript; RWD reviewed and revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Dai Ruiwu.

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Authors declare that no competing financial interest or conflict of interest exist.

Ethical approval

This study was approved by the ethics committee of the General Hospital of Western Theater Command (2021EC2-20). Every effort was made to minimize animal suffering in the study.

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Zhirong, Z., Li, H., Yiqun, H. et al. Enhancing or inhibiting apoptosis? The effects of ucMSC-Ex in the treatment of different degrees of traumatic pancreatitis. Apoptosis 27, 521–530 (2022). https://doi.org/10.1007/s10495-022-01732-1

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