Abstract.
Podocalyxin (PODXL) is a mucin protein of the CD34 family expressed in kidney glomerular podocytes, vascular endothelium, progenitor bone marrow and tumor cells. It is assumed that PODXL plays an anti-adherent role in kidney podocytes. CHO cells stably expressing human PODXL (CHO-PODXL) or human tumor cells (Tera-1) inherently expressing PODXL showed increased adherence to platelets. The adherence of cells was inhibited (70%) by blockers of platelet P-selectin, prevented by the soluble ectodomain of human PODXL (PODXL-Δ) or by the arginine-glycine-aspartate (RGDS) peptide and partially impeded by inhibition of integrin αVβ3/αVβ5, suggesting a coordinated action of P-selectin and integrins. Colocalization of platelet P-selectin and PODXL expressed on CHO cells was demonstrated by confocal immunofluorescence. No adherence to platelets was observed when PODXL was expressed in glycomutant CHO cells deficient in sialic acid.
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Received 14 August 2007; received after revision 12 September 2007; accepted 13 September 2007
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Larrucea, S., Butta, N., Rodriguez, R.B. et al. Podocalyxin enhances the adherence of cells to platelets. Cell. Mol. Life Sci. 64, 2965–2974 (2007). https://doi.org/10.1007/s00018-007-7374-6
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DOI: https://doi.org/10.1007/s00018-007-7374-6