Abstract
The regulation of cell cycle progression in normal mammalian cells is dependent on the presence of growth factors. In their absence, non-transformed cells will stop dividing and enter the quiescent state (G0). We show here that in Chinese hamster ovary cells, at least two serum-dependent points exist during G1 that lead to different cellular responses. The first point is located immediately after mitosis and is suggested to link with apoptosis. The second point is located late in G1, and probably corresponds with the ‘classic’ restriction point R. Cells depleted of serum after the first restriction point will not stop randomly in G1 but continue G1 progression until they reach the late restriction point, as marked by translocation of p42MAPkinase (ERK2) to the nucleus.
Similar content being viewed by others
Author information
Authors and Affiliations
Corresponding author
Additional information
Received 18 September 2003; received after revision 11 December 2003; accepted 19 December 2003
An erratum to this article is available at http://dx.doi.org/10.1007/s00018-017-2548-3.
Rights and permissions
About this article
Cite this article
Hullemann, E., Bijvelt, J.J.M., Verkleij, A.J. et al. Identification of a restriction point at the M/G1 transition in CHO cells. CMLS, Cell. Mol. Life Sci. 61, 600–609 (2004). https://doi.org/10.1007/s00018-003-3360-9
Issue Date:
DOI: https://doi.org/10.1007/s00018-003-3360-9