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STING-IFN-I pathway relieves incision induced acute postoperative pain via inhibiting the neuroinflammation in dorsal root ganglion of rats

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Abstract

Background

The purpose of this study was to study the effect of STING-IFN-I pathway on incision induced postoperative pain in rats and its possible mechanisms.

Methods

The pain thresholds were evaluated by measuring the mechanical withdrawal threshold and the thermal withdrawal latency. The satellite glial cell and macrophage of DRG were analyzed. The expression of STING, IFN-a, P-P65, iNOS, TNF-α, IL-1β and IL-6 in DRG was evaluated.

Results

The activation of STING-IFN-I pathway can reduce the mechanical hyperalgesia, thermal hyperalgesia, down-regulate the expression of P-P65, iNOS, TNF-α, IL-1β and IL-6, and inhibit the activation of satellite glial cell and macrophage in DRG.

Conclusions

The activation of STING-IFN-I pathway can alleviate incision induced acute postoperative pain by inhibiting the activation of satellite glial cell and macrophage, which reducing the corresponding neuroinflammation in DRG.

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Data availability

The authors declare that all data supporting the findings of this study are available within the article and the supplementary materials.

Abbreviations

WHO:

World Health Organization

DRG:

Dorsal root ganglion

IFN-I:

Type I interferon

EAE:

Experimental autoimmune encephalomyelitis

WB:

Western blotting

PTX:

Paclitaxel

CIPN:

Chemotherapy-induced peripheral neuropathy

CFA:

Complete Freund’s adjuvant

CCI:

Chronic constriction injury

PTPRD:

Protein tyrosine phosphatase receptor type D

RST:

Restraint stress

SNI:

Sciatic nerve injury

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Acknowledgements

This work was supported by the National Key Research and Development Program of China (Grant 2018YFC2001802 to X. Chen); National Natural Science Foundation (Grant 82071251 to X. Chen); Hubei Province Key Research and Development Program (Grant 2021BCA145 to X. Chen).

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Author notes

  1. Lulin Ma, Daling Deng have contributed equally to this work and share first authorship.

Authors and Affiliations

  1. Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Lulin Ma, Daling Deng, Tianhao Zhang, Wenjing Zhao, Shiqian Huang, Feng Xu, Yafeng Wang, Yuanyuan Ding, Kaixin Wang, Yanyan Zhang, Xinxin Yang & Xiangdong Chen

  2. Department of Anesthesiology, The Second Affiliated Hospital of University of South China, Hengyang, China

    Chengxi Liu

  3. Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China

    Lulin Ma, Daling Deng, Tianhao Zhang, Wenjing Zhao, Chengxi Liu, Shiqian Huang, Feng Xu, Yafeng Wang, Shuai Zhao, Yuanyuan Ding, Yan Huang, Kaixin Wang, Yanyan Zhang, Xinxin Yang, Song Cao & Xiangdong Chen

  4. Department of Pain Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi, China

    Shuai Zhao & Song Cao

  5. Department of Anesthesiology, The First People’s Hospital of Jiangxia District, Wuhan, China

    Yan Huang

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  1. Lulin Ma
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Contributions

This work was primarily conceived by XC and LM, data was collected by LM and DD, and analyzed by LM, DD, TZ and WZ. Manuscript was written by LM, DD, TZ, WZ, CL, SH, FX, YW, SZ, YD, YH, KW, XY, YZ, SC and XC. Figures were produced by LM. All authors reviewed the manuscript.

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Correspondence to Xiangdong Chen.

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Ma, L., Deng, D., Zhang, T. et al. STING-IFN-I pathway relieves incision induced acute postoperative pain via inhibiting the neuroinflammation in dorsal root ganglion of rats. Inflamm. Res. 72, 1551–1565 (2023). https://doi.org/10.1007/s00011-023-01764-6

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