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Immunoexpression of canonical Wnt and NF-κB signaling pathways in the temporomandibular joint of arthritic rats

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Abstract

Objective

To investigate the participation of canonical Wnt and NF-κB signaling pathways in an experimental model of chronic arthritis induced by methylated bovine serum albumin (mBSA) in rat temporomandibular joint (TMJ).

Materials and methods

Wistar rats were sensitized by mBSA+Complete Freund Adjuvant (CFA)/Incomplete Freund Adjuvant (IFA) on the first 14 days (1 ×/week). Subsequently, they received 1, 2 or 3 mBSA or saline solution injections into the TMJ (1 ×/week). Hypernociceptive threshold was assessed during the whole experimental period. 24 h after the mBSA injections, the TMJs were removed for histopathological and immunohistochemical analyses for TNF-α, IL-1β, NF-κB, RANKL, Wnt-10b, β-catenin and DKK1.

Results

The nociceptive threshold was significantly reduced after mBSA injections. An inflammatory infiltrate and thickening of the synovial membrane were observed only after mBSA booster injections. Immunolabeling of TNF-α, IL-1β and Wnt-10b was increased in the synovial membrane in arthritic groups. The immunoexpression of nuclear β-catenin was significantly higher only in the group that received 2 booster TMJ injections. However, NF-κB, RANKL and DKK1 immunoexpression were increased only in animals with 3 mBSA intra-articular injections.

Conclusion

Our results suggest that canonical Wnt and NF-κB signaling pathways participate in the hypernociception and inflammatory response in TMJ synovial membrane during the development of rheumatoid arthritis in rats.

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Acknowledgements

The authors gratefully acknowledge the technical assistance of Howard Lopes Ribeiro Júnior, Flávia de Araújo Silva and Adalberto Nascimento de Lima Júnior. This work was supported by grants from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.

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  1. Post Graduate Program in Morphofunctional Sciences, Department of Morphology, Federal University of Ceará, Rua Delmiro de Farias, S/N, Rodolfo Teófilo, Fortaleza, CE, CEP 60416-030, Brazil

    Luane Macêdo de Sousa, Conceição da Silva Martins, Karuza Maria Alves Pereira, Paula Goes & Delane Viana Gondim

  2. Post Graduate Program in Dentistry, Department of Clinical Dentistry, Federal University of Ceará, Fortaleza, CE, Brazil

    Joana Maria dos Santos Alves, Karuza Maria Alves Pereira, Paula Goes & Delane Viana Gondim

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  1. Luane Macêdo de Sousa
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de Sousa, L.M., dos Santos Alves, J.M., da Silva Martins, C. et al. Immunoexpression of canonical Wnt and NF-κB signaling pathways in the temporomandibular joint of arthritic rats. Inflamm. Res. 68, 889–900 (2019). https://doi.org/10.1007/s00011-019-01274-4

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