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RETRACTED ARTICLE: MiR-138-5p exacerbates hypoxia/reperfusion-induced heart injury through the inactivation of SIRT1-PGC-1α

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This article was retracted on 14 October 2020

This article has been updated

Abstract

Objectives

A drastic reduction in myocardial cell apoptosis plays a crucial role in the treatment/management of myocardial infarction, a major cardiovascular health challenge confronting the world, especially the Western world. Accumulating evidence indicates that the cardiotoxicity caused by the apoptotic machinery is partly regulated by miRNAs. The aim of this research is to investigate the role of miR-138-5p on hypoxia/reperfusion-induced heart injury.

Methods

The expression of miR-138-5p was determined in heart tissue from myocardial infarction patients and rats. Rats were transfection with a miR-138-5p inhibitor to silence miR-138-5p. The cardiac function of rats was detected via echocardiography. SIRT1 and PGC-1α expression in cardiac infarction was detected via quantitative Real-time PCR (qPCR) and Western blot analysis, while the TUNEL assay was used to determine myocardial apoptosis.

Results

Our observations showed that miR-138-5p expression was upregulated after the induction of myocardial infarction. The miR-138-5p inhibitor significantly improved cardiac function, increased the expression of SIRT1 and PGC-1α, and decreased the rate of myocardial apoptosis, whereas siRNA-SIRT1 reversed these protective effects.

Conclusions

In conclusion, our study demonstrated that miR-138-5p could promote cardiac ischemia injury via inhibition of the silent information regulator 1 and peroxisome proliferator-initiated receptor gamma and coactivator 1 alpha (SIRT1–PGC-1α) axis.

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Change history

  • 14 October 2020

    This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1007/s00011-020-01406-1

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Acknowledgements

This study was supported by the projects of the National Natural Science Foundation of China (NSFC) (Grant no. 814732117).

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Author notes

  1. Cuiping Wang and Xia Sun contributed equally to this work.

Authors and Affiliations

  1. Department of Cardiology, Affiliated Hospital of Jining Medical University, Jining, 272000, Shandong, People’s Republic of China

    Cuiping Wang, Xia Sun, Zhi Qiu & Anyong Chen

  2. Department of Clinical Laboratory, Affiliated Hospital of Jining Medical University, 89 Guhuai Road, Jining, 272000, Shandong, People’s Republic of China

    Anyong Chen

Authors
  1. Cuiping Wang
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  2. Xia Sun
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Contributions

AC designed research and wrote the paper; CW and XS performed research and analyzed data; ZQ helped with the qPCR experiments and data analysis.

Corresponding author

Correspondence to Anyong Chen.

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We declare no competing financial interests.

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Responsible Editor: John Di Battista.

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Wang, C., Sun, X., Qiu, Z. et al. RETRACTED ARTICLE: MiR-138-5p exacerbates hypoxia/reperfusion-induced heart injury through the inactivation of SIRT1-PGC-1α. Inflamm. Res. 68, 867–876 (2019). https://doi.org/10.1007/s00011-019-01268-2

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  • DOI: https://doi.org/10.1007/s00011-019-01268-2

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