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A novel N-acetyl-glucosamine lectin of Lonchocarpus araripensis attenuates acute cellular inflammation in mice

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Abstract

Objective and design

This study had investigated the anti-inflammatory activity of a seed lectin (LAL) isolated from Lonchocarpus araripensis.

Material/methods

LAL was purified by affinity chromatography (chitin column) and ion exchange chromatography (DEAE-Sephacel). In vitro LAL was tested for hemagglutinating activity against rabbit erythrocytes. In vivo LAL was assessed for the anti-inflammatory activity via intravenous injection (i.v.) in Swiss mice (25–30 g; n = 6/group) in models of paw edema and peritonitis. Statistical analysis: ANOVA (p < 0.05).

Results

LAL revealed two bands of 30 and 60 kDa (SDS-PAGE) and exhibited hemagglutinating activity. LAL (10 mg/kg) inhibited the paw edema (77 %) and vascular permeability (26 %) induced by carrageenan, and the paw edema induced by serotonin (80 %), bradykinin (49 %), sodium nitroprusside (83 %), TNF-α (75 %) and PGE2 (64 %). LAL also inhibited the neutrophil migration induced by fMLP (70 %) or carrageenan (69 %). The intravital microscopy showed that LAL inhibited rolling (83 %) and adhesion (70 %) of leukocytes. LAL anti-inflammatory effect was reversed by its association with N-acetyl-glucosamine. The nine-daily treatment with LAL (10 mg/kg; i.v.) showed no toxicity.

Conclusion

The novel N-acetyl-d-glucosamine-binding lectin isolated from L. araripensis seeds presents anti-inflammatory effect involving the lectin domain and the inhibition of 5-HT, BK, PGE2, NO, TNF-α and leukocyte rolling and adhesion.

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Acknowledgments

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP) and technical assistance of Gabriela F.O. Marques-Domingos are acknowledged.

Author information

Authors and Affiliations

  1. Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Paranjana, 1700, Itaperi, Fortaleza, CE, 60.740-000, Brazil

    Alana F. Pires, Natália V. F. C. Rodrigues & Ana Maria S. Assreuy

  2. Departamento de Morfologia e Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Rua Delmiro de Farias, s/n, Rodolfo Teófilo, Fortaleza, CE, 60.416-030, Brazil

    Pedro Marcos G. Soares, Ronaldo de Albuquerque Ribeiro & Karoline S. Aragão

  3. Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Av. Mister Hull, s/n, Fortaleza, CE, 60.451-970, Brazil

    Márcia M. Marinho, Mayara T. L. da Silva & Benildo S. Cavada

  4. Centro de Ciências da Saúde, Unidade Via Corpvs, Centro Universitário Estácio do Ceará, Rua Eliseu Uchoa Becco, 600, Fortaleza, CE, 60.810-270, Brazil

    Alana F. Pires

Authors
  1. Alana F. Pires
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  2. Natália V. F. C. Rodrigues
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  3. Pedro Marcos G. Soares
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  4. Ronaldo de Albuquerque Ribeiro
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  5. Karoline S. Aragão
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  6. Márcia M. Marinho
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  7. Mayara T. L. da Silva
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  8. Benildo S. Cavada
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  9. Ana Maria S. Assreuy
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Corresponding authors

Correspondence to Alana F. Pires or Ana Maria S. Assreuy.

Additional information

Responsible Editor: Mauro Teixeira.

Deceased: Ronaldo de Albuquerque Ribeiro.

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Pires, A.F., Rodrigues, N.V.F.C., Soares, P.M.G. et al. A novel N-acetyl-glucosamine lectin of Lonchocarpus araripensis attenuates acute cellular inflammation in mice. Inflamm. Res. 65, 43–52 (2016). https://doi.org/10.1007/s00011-015-0889-7

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  • DOI: https://doi.org/10.1007/s00011-015-0889-7

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