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The pentapeptide PLNPK inhibits systemic lupus erythematosus-associated renal damage

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Abstract

Objective

This study aimed to observe the therapeutic effect of pentapeptide PLNPK on systemic lupus erythematosus (SLE) in mice, and to study the inhibitory effect of PLNPK on activation of T cells in vivo.

Methods

Murine SLE-like chronic graft-versus-host disease (cGVHD) was induced. After treatment with PLNPK (100, 200, 400 μg/kg per day) for 70 days, serum blood urea nitrogen, creatine, total cholesterol, triglyeride and albumin were tested, and serum levels of anti-dsDNA and anti-histone antibodies were detected by ELISA. The pathological damage and IgG deposition in the kidney were identified. Concanavalin A (ConA)-induced T lymphocyte proliferation in SLE mice was also tested.

Results

PLNPK can reduce serum blood urea nitrogen, creatine, total cholesterol, triglyeride, and elevate serum albumin, and reduce levels of anti-dsDNA and anti-histone antibodies in the murine SLE model. Pathological damage and IgG deposition in the kidney were reduced in the PLNPK-treated group. PLNPK inhibited T lymphocyte infiltration in kidney tissues and ConA-induced T lymphocyte proliferation in SLE mice.

Conclusion

Our results demonstrate that PLNPK can suppress T cell function and reduce the production of autoantibodies, and may be a feasible and effective therapy in the SLE model.

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Acknowledgments

This work was supported by Grant sponsor: National Basic Research Program (973 Program, China) (Grant number: 2009CB918903); and Natural Science Foundation of Tianjin (Grant number: 09JCZDJC19700); and Cooperation Project in Industry, Education and Research of Guangdong Province (Grant number: 2009B090300430).

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Authors and Affiliations

  1. Department of Immunology, Tianjin Medical University, 22 Qixiangtai Road, Tianjin, 300070, China

    Jun-qiang Lv, Wen Zhang, Song Wang, Lin Zhao, Rui Ma, Jin-wei Hu, Li-juan Wang, Jie Meng, Chun-lei Zhou, Gang Lin, Rong Lu & Zhi Yao

  2. Shenzhen Kangzhe Pharmaceutical, No. 11, Langsan Road, North Zone of Technology Park, Nanshan, Shenzhen, 518029, Guangdong, China

    Gang Lin & Rong Lu

  3. Key Laboratory of Immuno Microenvironment and Disease of the Educational Ministry of China, Tianjin, 300070, China

    Zhi Yao

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  1. Jun-qiang Lv
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  2. Wen Zhang
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  3. Song Wang
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  4. Lin Zhao
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  5. Rui Ma
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  7. Li-juan Wang
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  9. Chun-lei Zhou
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  10. Gang Lin
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  11. Rong Lu
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Corresponding author

Correspondence to Zhi Yao.

Additional information

Responsible Editor: John Di Battista.

J. Lv and W. Zhang contributed equally to this paper.

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Lv, Jq., Zhang, W., Wang, S. et al. The pentapeptide PLNPK inhibits systemic lupus erythematosus-associated renal damage. Inflamm. Res. 59, 1081–1089 (2010). https://doi.org/10.1007/s00011-010-0228-y

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  • DOI: https://doi.org/10.1007/s00011-010-0228-y

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