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Fabrication of reduced graphene oxide/ruthenium oxide modified graphite electrode for voltammetric determination of tryptophan

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Abstract

A simple and sensitive sensor based on graphite electrode (G) modified by reduced graphene oxide-ruthenium oxide (rGO-RuO2) composite was developed through one-step electrodeposition. The fabricated electrode was well characterized by FE-SEM, EDX, and electrochemical technique and employed for electrochemical determination of tryptophan (Trp) in pharmaceutical sample. The electrooxidation of tryptophan was studied on modified G electrode using cyclic voltammetry and differential pulse voltammetry (DPV) as diagnostic techniques. It was found that the oxidation peak current of tryptophan on the modified G electrode was excellently enhanced compared to that obtained on the bare G electrode. Under optimum conditions the sensor provides linear DPV responses in the range of 0.5–240 μM in phosphate buffer solution of pH 7 with a detection limit of 0.236 μM. The proposed sensor was successfully applied for monitoring of Trp in pharmaceutical sample and satisfactory results were obtained.

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Acknowledgements

One of the authors, Mahesh Bhaskar Hegde, would like to acknowledge Karnataka Science and Technology Promotion Society (KSTePS), Karnataka, India, for financial support.

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Authors and Affiliations

  1. Department of Studies in Chemistry, University of Mysore, Manasagangothri, Mysuru, Karnataka, 570006, India

    Mahesh Bhaskar Hegde, Kikkeri Narasimha Shetty Mohana, Ambale Murthy Madhusudhana & Ningappa Kumara Swamy

  2. Department of Chemistry, School of Chemical Science, Kuvempu University, Shankaraghatta, Karnataka, 577451, India

    M. M. Vinay & Y. Arthoba Nayaka

  3. Department of Chemistry, Sri Jayachamarajendra College of Engineering, Mysuru, Karnataka, 570006, India

    Ningappa Kumara Swamy

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  1. Mahesh Bhaskar Hegde
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  2. Kikkeri Narasimha Shetty Mohana
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  3. Ambale Murthy Madhusudhana
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  4. M. M. Vinay
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Correspondence to Kikkeri Narasimha Shetty Mohana.

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Hegde, M.B., Mohana, K.N.S., Madhusudhana, A.M. et al. Fabrication of reduced graphene oxide/ruthenium oxide modified graphite electrode for voltammetric determination of tryptophan. Graphene and 2D Materials Technol 6, 25–34 (2021). https://doi.org/10.1007/s41127-021-00042-8

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