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Electron tomography of biological samples

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Abstract

Electron tomography allows computing three-dimensional (3D) reconstructions of objects from their projections recorded at several angles. Combined with transmission electron microscopy, electron tomography has contributed greatly to the understanding of subcellular structures and organelles. Performed on frozen-hydrated samples, electron tomography has yielded useful information about complex biological structures. Combined with energy filtered transmission electron microscopy (EFTEM) it can be used to analyze the spatial distribution of chemical elements in biological or material sciences samples. In the present review, we present an overview of the requirements, applications, and perspectives of electron tomography in structural biology.

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REFERENCES

  1. S. Carlo ParticleDe C. El-Bez C. Alvarez Rua J. Borge J. Dubochet (2002) J. Struct. Biol. 138 216–226

    Google Scholar 

  2. M. Adrian J. Dubochet S. D. Fuller J. R. Harris (1998) Micron 29 145–160

    Google Scholar 

  3. J. Lepault J. Dubochet (1986) Meth. Enzymol. 127 719–730

    Google Scholar 

  4. J. Ruprecht J. Nield (2001) Progr. Biophys. Mol. Biol. 75 121–164

    Google Scholar 

  5. J. Frank (1989) Electron. Microsc. Rev. 2 53–74

    Google Scholar 

  6. W. Baumeister (2002) Curr. Opin. Struct. Biol. 12 679–684

    Google Scholar 

  7. W. Baumeister R. Grimm J. Walz (1999) Trends. Cell. Biol. 9 81–85

    Google Scholar 

  8. J. Frank T. Wagenknecht B. F. McEwen M. Marko C. E. Hsieh C. A. Mannella (2002) J. Struct. Biol. 138 85–91

    Google Scholar 

  9. C. E. Hsieh M. Marko J. Frank C. Mannella (2002) J. Struct. Biol. 138 63–73

    Google Scholar 

  10. B. F. McEwen M. Marko C. E. Hsieh C. Mannella (2002) J. Struct. Biol. 138 47–57

    Google Scholar 

  11. B. F. McEwen J. Frank (2001) Curr. Opin. Neurobiol. 11 594–600

    Google Scholar 

  12. J. Frank (1992) Electron Tomography.Three Dimensional Imaging with the Transmission Electron Microscope Plenum Press New York

    Google Scholar 

  13. B. McEwen M. Marko (2001) J. Histochem. Cytochem. 49 553–564

    Google Scholar 

  14. M. S. Ladinsky C. C. Wu S. McIntosh J. R. McIntosh K. E. Howell (2002) Mol. Biol. Cell. 13 2810–2825

    Google Scholar 

  15. C. A. Mannella (2001) Meth. Cell Biol. 65 245–256

    Google Scholar 

  16. O’Toole, E. T., Giddings, T. H., McIntosh, J. R., and Dutcher, S. K. (2003) Mol. Biol. Cell., 14, 2999–3012.

    Google Scholar 

  17. B. Shillito A. J. Koster J. Walz W. Baumeister (1996) Biol.Cell 88 5–13

    Google Scholar 

  18. J. Bohm O. Lambert A. S. Frangakis L. Letellier W. Baumeister J. L. Rigaud (2001) Curr. Biol. 11 1168–1175

    Google Scholar 

  19. R. A. Horowitz A. J. Koster J. Walz C. L. Woodcock (1997) J. Struct. Biol. 120 353–362

    Google Scholar 

  20. M. Moritz M. B. Braunfeld J. W. Sedat B. Alberts D. A. Agard (1995) Nature 378 638–640

    Google Scholar 

  21. M. Moritz M. B. Braunfeld V. Guenebaut J. Heuser D. A. Agard (2000) Nat. Cell Biol. 2 365–370

    Google Scholar 

  22. R. W. Horne (1978) J. Microsc. 113 241–256

    Google Scholar 

  23. J. Marle ParticleVan A. Dietrich K. Jonges R. Jonges E. de Moor A. Vink P. Boon H. Veen Particlevan (1995) Microsc. Res. Tech. 31 311–316

    Google Scholar 

  24. J. Dubochet (1995) Trends Cell Biol. 5 366–368

    Google Scholar 

  25. O. Medalia D. Typke R. Hegerl M. Angenitzki J. Sperling R. Sperling (2002) J. Struct. Biol. 138 74–84

    Google Scholar 

  26. V. R. Matias A. Al Amoudi J. Dubochet T. J. Beveridge (2003) J. Bacteriol. 185 6112–6118

    Google Scholar 

  27. J. Dubochet N. Sartori Blanc (2001) Micron 32 91–99

    Google Scholar 

  28. A. Al Amoudi J. Dubochet H. Gnaegi W. Luthi D. Studer (2003) J. Microsc. 212 26–33

    Google Scholar 

  29. R. M. Glaeser K. A. Taylor (1980) J. Microsc. 112 127–138

    Google Scholar 

  30. P. K. Luther (2001) Eur.Microsc.Analysis 70 7–9

    Google Scholar 

  31. A. J. Koster R. Grimm D. Typke R. Hegerl A. Stoschek J. Walz W. Baumeister (1997) J. Struct. Biol. 120 276–308

    Google Scholar 

  32. C. Messaoudi T. Boudier J. P. Lechaire J. L. Rigaud H. Delacroix F. Gaill S. Marco (2003) Biol.Cell 95 393–398

    Google Scholar 

  33. U. Ziese W. J. Geerts T. P. Krift Particlevan der A. J. Verkleij A. J. Koster (2003) J. Microsc. 211 179–185

    Google Scholar 

  34. Q. S. Zheng M. B. Braunfeld J. W. Sedat D. A. Agard (2004) J. Struct. Biol. 147 91–101

    Google Scholar 

  35. U. Ziese A. H. Janssen J. L. Murk W. J. Geerts T. Krift Particlevan der A. J. Verkleij A. J. Koster (2002) J. Microsc. 205 187–200

    Google Scholar 

  36. P. Penczek M. Radermacher J. Frank (1992) Ultramicroscopy 40 33–53

    Google Scholar 

  37. S. Marco M. Chagoyen L. G. Fraga J. M. Carazo J. L. Carrascosa (1996) Ultramicroscopy 66 5–10

    Google Scholar 

  38. J. J. Fernandez A. F. Lawrence J. Roca I. Garcia M. H. Ellisman J. M. Carazo (2002) J. Struct. Biol. 138 6–20

    Google Scholar 

  39. R. Grimm M. Barmann W. Hackl D. Typke E. Sackmann W. Baumeister (1997) Biophys. J. 72 482–489

    Google Scholar 

  40. G. Harauz M. Heel Particlevan (1986) Optik 73 146–156

    Google Scholar 

  41. W. O. Saxton W. Baumeister (1982) J. Microsc. 127 127–138

    Google Scholar 

  42. M. Heel ParticleVan (1987) Ultramicroscopy 21 95–100

    Google Scholar 

  43. M. Kessel M. Radermacher J. Frank (1985) J. Microsc. 139 63–74

    Google Scholar 

  44. M. Unser B. L. Trus J. Frank A. C. Steven (1989) Ultramicroscopy 30 429–433

    Google Scholar 

  45. P. A. Penczek (2002) J. Struct. Biol. 138 34–46

    Google Scholar 

  46. Unser, M., Sorzano, C. O., Thevenaz, P., Joni, S., El Bez, C., de Carlo, S., Conway, J., and Trus, B. L. (2004) J. Struct. Biol., in press.

  47. L. G. Ofverstedt K. Zhang L. A. Isaksson G. Bricogne U. Skoglund (1997) J. Struct. Biol. 120 329–342

    Google Scholar 

  48. R. Marabini J. M. Carazo (1994) Biophys. J. 66 1804–1814

    Google Scholar 

  49. A. Pascual Montano L. E. Donate M. Valle M. Barcena R. D. Pascual Marqui J. M. Carazo (2001) J. Struct. Biol. 133 233–245

    Google Scholar 

  50. A. Pascual Montano K. A. Taylor H. Winkler R. D. Pascual Marqui J. M. Carazo (2002) J. Struct. Biol. 138 114–122

    Google Scholar 

  51. J. A. Velazquez Muriel C. O. Sorzano J. J. Fernandez J. M. Carazo (2003) Ultramicroscopy 96 17–35

    Google Scholar 

  52. B. Sander M. M. Golas H. Stark (2003) J. Struct. Biol. 142 392–401

    Google Scholar 

  53. J. Bohm A. S. Frangakis R. Hegerl S. Nickell D. Typke W. Baumeister (2002) Proc.Natl.Acad.Sci.USA 99 14153–14158

    Google Scholar 

  54. A. S. Frangakis F. Forster (2004) Curr. Opin. Struct. Biol. 14 325–331

    Google Scholar 

  55. W. Wriggers S. Birmanns (2001) J. Struct. Biol. 133 193–202

    Google Scholar 

  56. J. Navaza J. Lepault F. A. Rey C. Alvarez Rua J. Borge (2002) Acta Crystallogr. D. Biol. Crystallogr. 58 1820–1825

    Google Scholar 

  57. J. M. Robinson T. Takizawa D. D. Vandre (2000) J. Microsc. 199 163–179

    Google Scholar 

  58. T. Cheutin M. F. O’Donohue A. Beorchia C. Klein H. Kaplan D. Ploton (2003) J. Histochem. Cytochem. 51 1411–1423

    Google Scholar 

  59. G. Mobus R. C. Doole B. J. Inkson (2003) Ultramicroscopy 96 433–451

    Google Scholar 

  60. P. A. Midgley M. Weyland (2003) Ultramicroscopy 96 413–431

    Google Scholar 

  61. M. Weyland P. A. Midgley (2003) Microsc. Microanal. 9 542–555

    Google Scholar 

  62. O’Toole, E. T., Winey, M., McIntosh, J. R., and Mastronarde, D. N. (2002) Meth. Enzymol., 351, 81–95.

    Google Scholar 

  63. A. S. Frangakis R. Hegerl (2002) J. Struct. Biol. 138 105–113

    Google Scholar 

  64. A. Stoschek R. Hegerl (1997) J. Struct. Biol. 120 257–265

    Google Scholar 

  65. A. S. Frangakis R. Hegerl (2001) J. Struct. Biol. 135 239–250

    Google Scholar 

  66. J. J. Fernandez S. Li (2003) J. Struct. Biol. 144 152–161

    Google Scholar 

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Authors and Affiliations

  1. Institut Curie, Section Recherche, UMR-CNRS 168 et LRC-CEA 34V 11, 75005, rue Pierre et Marie Curie, Paris, France

    S. Marco, T. Boudier, C. Messaoudi & J.-L. Rigaud

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  1. S. Marco
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  2. T. Boudier
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  3. C. Messaoudi
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  4. J.-L. Rigaud
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Correspondence to S. Marco.

Additional information

Translated from Biokhimiya, Vol. 69, No. 11, 2004, pp. 1497–1505.

Original Russian Text Copyright © 2004 by Marco, Boudier, Messaoudi, Rigaud

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Marco, S., Boudier, T., Messaoudi, C. et al. Electron tomography of biological samples. Biochemistry (Moscow) 69, 1219–1225 (2004). https://doi.org/10.1007/PL00021757

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